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Deep subsurface mine stalactites trap endemic fissure fluid Archaea, Bacteria, and Nematoda possibly originating from ancient seas.

Borgonie G, Linage-Alvarez B, Ojo A, Shivambu S, Kuloyo O, Cason ED, Maphanga S, Vermeulen JG, Litthauer D, Ralston CD, Onstott TC, Sherwood-Lollar B, Van Heerden E - Front Microbiol (2015)

Bottom Line: Nematoda were clearly identified between these layers confirming that bacteria and nematodes live inside the stalactites and not only in the central straw.Surprisingly, several Bacteria showing highest sequence identities to marine species were identified.Our results indicate stalactites are suitable for biodiversity recovery and act as natural traps for microorganisms in the fissure water long after the water that formed the stalactite stopped flowing.

View Article: PubMed Central - PubMed

Affiliation: Extreme Life Isyensya Gentbrugge, Belgium ; Department of Biotechnology, University of the Free State Bloemfontein, South Africa.

ABSTRACT
Stalactites (CaCO3 and salt) from water seeps are frequently encountered in ceilings of mine tunnels whenever they intersect water-bearing faults or fractures. To determine whether stalactites could be mineralized traps for indigenous fracture water microorganisms, we analyzed stalactites collected from three different mines ranging in depth from 1.3 to 3.1 km. During sampling in Beatrix gold mine (1.4 km beneath the surface), central South Africa, CaCO3 stalactites growing on the mine tunnel ceiling were collected and observed, in two cases, to contain a living obligate brackish water/marine nematode species, Monhystrella parvella. After sterilization of the outer surface, mineral layers were physically removed from the outside to the interior, and DNA extracted. Based upon 16S and 18S rRNA gene sequencing, Archaea, Bacteria, and Eukarya in different combinations were detected for each layer. Using CT scan and electron microscopy the inner structure of CaCO3 and salt stalactites were analyzed. CaCO3 stalactites show a complex pattern of lamellae carrying bacterially precipitated mineral structures. Nematoda were clearly identified between these layers confirming that bacteria and nematodes live inside the stalactites and not only in the central straw. Salt stalactites exhibit a more uniform internal structure. Surprisingly, several Bacteria showing highest sequence identities to marine species were identified. This, together with the observation that the nematode M. parvella recovered from Beatrix gold mine stalactite can only survive in a salty environment makes the origin of the deep subsurface colonization enigmatic. The possibility of a Permian origin of fracture fluids is discussed. Our results indicate stalactites are suitable for biodiversity recovery and act as natural traps for microorganisms in the fissure water long after the water that formed the stalactite stopped flowing.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of the Bacteria extracted from the salt stalactites.
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Figure 2: Phylogenetic tree of the Bacteria extracted from the salt stalactites.

Mentions: The bacterial phylogenetic diversity is represented by seven orders (Figure 2). Sphingobacteriales, Thermales, Burkholderiales, Alteromonadales, Enterobacteriales, Oceanospirillales, Bacillales. The largest represented groups were from the Orders Enterobacteriales, Burkholderiales, and Oceanospirillales. Significant was the identification of most likely marine Bacteria. The Order Oceanospirillales included one sequence that shared the closest identity 92% to one uncultured bacterium (SGUS738) identified from coral fragments taken in Panama. Two additional sequences clearly linked to Gamma-proteobacteria and Alcanivorax sp. In the Order Alteromonadales the sequence was closely related to Bowmanella as well as an uncultured marine bacterium. Attempts to culture Archaea and Bacteria from the layered extracts failed on three attempts.


Deep subsurface mine stalactites trap endemic fissure fluid Archaea, Bacteria, and Nematoda possibly originating from ancient seas.

Borgonie G, Linage-Alvarez B, Ojo A, Shivambu S, Kuloyo O, Cason ED, Maphanga S, Vermeulen JG, Litthauer D, Ralston CD, Onstott TC, Sherwood-Lollar B, Van Heerden E - Front Microbiol (2015)

Phylogenetic tree of the Bacteria extracted from the salt stalactites.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585192&req=5

Figure 2: Phylogenetic tree of the Bacteria extracted from the salt stalactites.
Mentions: The bacterial phylogenetic diversity is represented by seven orders (Figure 2). Sphingobacteriales, Thermales, Burkholderiales, Alteromonadales, Enterobacteriales, Oceanospirillales, Bacillales. The largest represented groups were from the Orders Enterobacteriales, Burkholderiales, and Oceanospirillales. Significant was the identification of most likely marine Bacteria. The Order Oceanospirillales included one sequence that shared the closest identity 92% to one uncultured bacterium (SGUS738) identified from coral fragments taken in Panama. Two additional sequences clearly linked to Gamma-proteobacteria and Alcanivorax sp. In the Order Alteromonadales the sequence was closely related to Bowmanella as well as an uncultured marine bacterium. Attempts to culture Archaea and Bacteria from the layered extracts failed on three attempts.

Bottom Line: Nematoda were clearly identified between these layers confirming that bacteria and nematodes live inside the stalactites and not only in the central straw.Surprisingly, several Bacteria showing highest sequence identities to marine species were identified.Our results indicate stalactites are suitable for biodiversity recovery and act as natural traps for microorganisms in the fissure water long after the water that formed the stalactite stopped flowing.

View Article: PubMed Central - PubMed

Affiliation: Extreme Life Isyensya Gentbrugge, Belgium ; Department of Biotechnology, University of the Free State Bloemfontein, South Africa.

ABSTRACT
Stalactites (CaCO3 and salt) from water seeps are frequently encountered in ceilings of mine tunnels whenever they intersect water-bearing faults or fractures. To determine whether stalactites could be mineralized traps for indigenous fracture water microorganisms, we analyzed stalactites collected from three different mines ranging in depth from 1.3 to 3.1 km. During sampling in Beatrix gold mine (1.4 km beneath the surface), central South Africa, CaCO3 stalactites growing on the mine tunnel ceiling were collected and observed, in two cases, to contain a living obligate brackish water/marine nematode species, Monhystrella parvella. After sterilization of the outer surface, mineral layers were physically removed from the outside to the interior, and DNA extracted. Based upon 16S and 18S rRNA gene sequencing, Archaea, Bacteria, and Eukarya in different combinations were detected for each layer. Using CT scan and electron microscopy the inner structure of CaCO3 and salt stalactites were analyzed. CaCO3 stalactites show a complex pattern of lamellae carrying bacterially precipitated mineral structures. Nematoda were clearly identified between these layers confirming that bacteria and nematodes live inside the stalactites and not only in the central straw. Salt stalactites exhibit a more uniform internal structure. Surprisingly, several Bacteria showing highest sequence identities to marine species were identified. This, together with the observation that the nematode M. parvella recovered from Beatrix gold mine stalactite can only survive in a salty environment makes the origin of the deep subsurface colonization enigmatic. The possibility of a Permian origin of fracture fluids is discussed. Our results indicate stalactites are suitable for biodiversity recovery and act as natural traps for microorganisms in the fissure water long after the water that formed the stalactite stopped flowing.

No MeSH data available.


Related in: MedlinePlus