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Colonization of root cells and plant growth promotion by Piriformospora indica occurs independently of plant common symbiosis genes.

Banhara A, Ding Y, Kühner R, Zuccaro A, Parniske M - Front Plant Sci (2015)

Bottom Line: Here we show that intracellular colonization of root cells and intracellular sporulation by P. indica occurred in CSG mutants of the legume Lotus japonicus and in Arabidopsis thaliana, which belongs to the Brassicaceae, a family that has lost the ability to form AM as well as a core set of CSGs.A. thaliana mutants of homologs of CSGs (HCSGs) interacted with P. indica similar to the wild-type.Moreover, increased biomass of A. thaliana evoked by P. indica was unaltered in HCSG mutants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, Institute of Genetics, University of Munich Martinsried, Germany.

ABSTRACT
Arbuscular mycorrhiza (AM) fungi (Glomeromycota) form symbiosis with and deliver nutrients via the roots of most angiosperms. AM fungal hyphae are taken up by living root epidermal cells, a program which relies on a set of plant common symbiosis genes (CSGs). Plant root epidermal cells are also infected by the plant growth-promoting fungus Piriformospora indica (Basidiomycota), raising the question whether this interaction relies on the AM-related CSGs. Here we show that intracellular colonization of root cells and intracellular sporulation by P. indica occurred in CSG mutants of the legume Lotus japonicus and in Arabidopsis thaliana, which belongs to the Brassicaceae, a family that has lost the ability to form AM as well as a core set of CSGs. A. thaliana mutants of homologs of CSGs (HCSGs) interacted with P. indica similar to the wild-type. Moreover, increased biomass of A. thaliana evoked by P. indica was unaltered in HCSG mutants. We conclude that colonization and growth promotion by P. indica are independent of the CSGs and that AM fungi and P. indica exploit different host pathways for infection.

No MeSH data available.


Related in: MedlinePlus

Colonization of L. japonicus root cells by P. indica. Wild-type (Gifu) and the indicated common symbiosis mutants were analyzed 7 dpi with P. indica chlamydospores. Intracellular hyphae were present in all genotypes (examples are indicated by asterisks). Roots were cleared and double stained with propidium iodide (red), for cell wall visualization, and WGA-AF488 (green), for fungal structures. Scale bar: 25 μm.
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Figure 1: Colonization of L. japonicus root cells by P. indica. Wild-type (Gifu) and the indicated common symbiosis mutants were analyzed 7 dpi with P. indica chlamydospores. Intracellular hyphae were present in all genotypes (examples are indicated by asterisks). Roots were cleared and double stained with propidium iodide (red), for cell wall visualization, and WGA-AF488 (green), for fungal structures. Scale bar: 25 μm.

Mentions: We investigated whether the classical CSGs are involved in the interaction between roots of the legume L. japonicus and P. indica. In root epidermal cells of L. japonicus wild-type (ecotype “Gifu”), and in CSG mutants intracellular hyphae were detected at 7 dpi (Figure 1) and sporulation at 14 dpi (Supplementary Figure 1), evidencing that P. indica entered host cells and successfully completed its life cycle in all genotypes tested. These observations indicate that the CSGs are not required for the successful infection of host cells by P. indica.


Colonization of root cells and plant growth promotion by Piriformospora indica occurs independently of plant common symbiosis genes.

Banhara A, Ding Y, Kühner R, Zuccaro A, Parniske M - Front Plant Sci (2015)

Colonization of L. japonicus root cells by P. indica. Wild-type (Gifu) and the indicated common symbiosis mutants were analyzed 7 dpi with P. indica chlamydospores. Intracellular hyphae were present in all genotypes (examples are indicated by asterisks). Roots were cleared and double stained with propidium iodide (red), for cell wall visualization, and WGA-AF488 (green), for fungal structures. Scale bar: 25 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585188&req=5

Figure 1: Colonization of L. japonicus root cells by P. indica. Wild-type (Gifu) and the indicated common symbiosis mutants were analyzed 7 dpi with P. indica chlamydospores. Intracellular hyphae were present in all genotypes (examples are indicated by asterisks). Roots were cleared and double stained with propidium iodide (red), for cell wall visualization, and WGA-AF488 (green), for fungal structures. Scale bar: 25 μm.
Mentions: We investigated whether the classical CSGs are involved in the interaction between roots of the legume L. japonicus and P. indica. In root epidermal cells of L. japonicus wild-type (ecotype “Gifu”), and in CSG mutants intracellular hyphae were detected at 7 dpi (Figure 1) and sporulation at 14 dpi (Supplementary Figure 1), evidencing that P. indica entered host cells and successfully completed its life cycle in all genotypes tested. These observations indicate that the CSGs are not required for the successful infection of host cells by P. indica.

Bottom Line: Here we show that intracellular colonization of root cells and intracellular sporulation by P. indica occurred in CSG mutants of the legume Lotus japonicus and in Arabidopsis thaliana, which belongs to the Brassicaceae, a family that has lost the ability to form AM as well as a core set of CSGs.A. thaliana mutants of homologs of CSGs (HCSGs) interacted with P. indica similar to the wild-type.Moreover, increased biomass of A. thaliana evoked by P. indica was unaltered in HCSG mutants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, Institute of Genetics, University of Munich Martinsried, Germany.

ABSTRACT
Arbuscular mycorrhiza (AM) fungi (Glomeromycota) form symbiosis with and deliver nutrients via the roots of most angiosperms. AM fungal hyphae are taken up by living root epidermal cells, a program which relies on a set of plant common symbiosis genes (CSGs). Plant root epidermal cells are also infected by the plant growth-promoting fungus Piriformospora indica (Basidiomycota), raising the question whether this interaction relies on the AM-related CSGs. Here we show that intracellular colonization of root cells and intracellular sporulation by P. indica occurred in CSG mutants of the legume Lotus japonicus and in Arabidopsis thaliana, which belongs to the Brassicaceae, a family that has lost the ability to form AM as well as a core set of CSGs. A. thaliana mutants of homologs of CSGs (HCSGs) interacted with P. indica similar to the wild-type. Moreover, increased biomass of A. thaliana evoked by P. indica was unaltered in HCSG mutants. We conclude that colonization and growth promotion by P. indica are independent of the CSGs and that AM fungi and P. indica exploit different host pathways for infection.

No MeSH data available.


Related in: MedlinePlus