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Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus

SDS-PAGE of purified fractions of biosurfactant from strain 3B-2 (Lanes 1–3: samples collected on DEAE-FF column, corresponding to three protein peaks in Figure 5; lanes 4 and 5: samples collected on S-100 column, corresponding to two protein peaks in Figure 6).
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Figure 7: SDS-PAGE of purified fractions of biosurfactant from strain 3B-2 (Lanes 1–3: samples collected on DEAE-FF column, corresponding to three protein peaks in Figure 5; lanes 4 and 5: samples collected on S-100 column, corresponding to two protein peaks in Figure 6).

Mentions: Gel electrophoresis revealed that in addition to two strong bands (i.e., B1 and B2), there were other miscellaneous protein bands in the samples collected from the DEAE-FF column (Figure 7, lanes 1–3). Clearly, the three samples were not completely purified on the DEAE-FF column. Samples collected from the Sephacryl S-100 column only showed a single clear band each (Figure 7, lanes 4 and 5), which was indicative of their high purity. The molecular weights of B11 and B12 were ∼40 and 22 kDa, respectively.


Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

SDS-PAGE of purified fractions of biosurfactant from strain 3B-2 (Lanes 1–3: samples collected on DEAE-FF column, corresponding to three protein peaks in Figure 5; lanes 4 and 5: samples collected on S-100 column, corresponding to two protein peaks in Figure 6).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585178&req=5

Figure 7: SDS-PAGE of purified fractions of biosurfactant from strain 3B-2 (Lanes 1–3: samples collected on DEAE-FF column, corresponding to three protein peaks in Figure 5; lanes 4 and 5: samples collected on S-100 column, corresponding to two protein peaks in Figure 6).
Mentions: Gel electrophoresis revealed that in addition to two strong bands (i.e., B1 and B2), there were other miscellaneous protein bands in the samples collected from the DEAE-FF column (Figure 7, lanes 1–3). Clearly, the three samples were not completely purified on the DEAE-FF column. Samples collected from the Sephacryl S-100 column only showed a single clear band each (Figure 7, lanes 4 and 5), which was indicative of their high purity. The molecular weights of B11 and B12 were ∼40 and 22 kDa, respectively.

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus