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Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus

Response surface plots of biosurfactant production from strain 3B-2. (A) Interactive effect of lactose and yeast extract with 20 g/L NaCl; (B) Interactive effect of lactose and NaCl with 0.6% yeast; (C) Interactive effect of yeast extract and NaCl with 0.5% lactose.
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Figure 4: Response surface plots of biosurfactant production from strain 3B-2. (A) Interactive effect of lactose and yeast extract with 20 g/L NaCl; (B) Interactive effect of lactose and NaCl with 0.6% yeast; (C) Interactive effect of yeast extract and NaCl with 0.5% lactose.

Mentions: Response surface plots of biosurfactant production from strain 3B-2 (Figure 4) were generated from the quadratic regression equation. The shape of the response surface was analyze to evaluate the interactive and individual effects of relevant factors on the response value. The contour of the model was nearly horizontal, rather than elliptical, possibly because the interactive effect of relevant factors was relatively weak (Reddy et al., 2008).


Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Response surface plots of biosurfactant production from strain 3B-2. (A) Interactive effect of lactose and yeast extract with 20 g/L NaCl; (B) Interactive effect of lactose and NaCl with 0.6% yeast; (C) Interactive effect of yeast extract and NaCl with 0.5% lactose.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585178&req=5

Figure 4: Response surface plots of biosurfactant production from strain 3B-2. (A) Interactive effect of lactose and yeast extract with 20 g/L NaCl; (B) Interactive effect of lactose and NaCl with 0.6% yeast; (C) Interactive effect of yeast extract and NaCl with 0.5% lactose.
Mentions: Response surface plots of biosurfactant production from strain 3B-2 (Figure 4) were generated from the quadratic regression equation. The shape of the response surface was analyze to evaluate the interactive and individual effects of relevant factors on the response value. The contour of the model was nearly horizontal, rather than elliptical, possibly because the interactive effect of relevant factors was relatively weak (Reddy et al., 2008).

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus