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Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus

Maximum-likelihood tree based on 16S rDNA gene sequence of strain 3B-2 isolated from Bohai Bay sediment (1169 bp) and those of known relatives retrieved from the GenBank database (accession No. in brackets). Bootstrap values shown at nodes for frequencies at or above a 50% threshold (per 1000 trials bootstrap resampling). Rhodobacter capsulatus used as outgroup. Bar indicates 0.05% sequence variance.
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Figure 2: Maximum-likelihood tree based on 16S rDNA gene sequence of strain 3B-2 isolated from Bohai Bay sediment (1169 bp) and those of known relatives retrieved from the GenBank database (accession No. in brackets). Bootstrap values shown at nodes for frequencies at or above a 50% threshold (per 1000 trials bootstrap resampling). Rhodobacter capsulatus used as outgroup. Bar indicates 0.05% sequence variance.

Mentions: A phylogenetic tree was constructed based on 16S rDNA sequences of strain 3B-2 and its close relatives retrieved from the GenBank database (Figure 2). In the maximum-likelihood tree, 3B-2 was clustered with most known strains of Vibrio sp. The result of phylogenetic analysis agreed with those of phenotypic tests. Strain 3B-2 was named Vibrio sp. 3B-2 and deposited in the China Center for Type Culture Collection under accession number CJ11052.


Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Maximum-likelihood tree based on 16S rDNA gene sequence of strain 3B-2 isolated from Bohai Bay sediment (1169 bp) and those of known relatives retrieved from the GenBank database (accession No. in brackets). Bootstrap values shown at nodes for frequencies at or above a 50% threshold (per 1000 trials bootstrap resampling). Rhodobacter capsulatus used as outgroup. Bar indicates 0.05% sequence variance.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585178&req=5

Figure 2: Maximum-likelihood tree based on 16S rDNA gene sequence of strain 3B-2 isolated from Bohai Bay sediment (1169 bp) and those of known relatives retrieved from the GenBank database (accession No. in brackets). Bootstrap values shown at nodes for frequencies at or above a 50% threshold (per 1000 trials bootstrap resampling). Rhodobacter capsulatus used as outgroup. Bar indicates 0.05% sequence variance.
Mentions: A phylogenetic tree was constructed based on 16S rDNA sequences of strain 3B-2 and its close relatives retrieved from the GenBank database (Figure 2). In the maximum-likelihood tree, 3B-2 was clustered with most known strains of Vibrio sp. The result of phylogenetic analysis agreed with those of phenotypic tests. Strain 3B-2 was named Vibrio sp. 3B-2 and deposited in the China Center for Type Culture Collection under accession number CJ11052.

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus