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Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus

Optimization of medium composition for biosurfactant production using the analytical hierarchy process.
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Figure 1: Optimization of medium composition for biosurfactant production using the analytical hierarchy process.

Mentions: In the AHP model, the top level (the goal) is the optimal medium composition (or operating factors). At the second level, there are four criteria: the biomass (OD600), the protein concentration, the surface tension, and the diameter of clear zone. At the third level, there are n alternatives that must be compared and assessed. An example of the AHP model for a case study of medium composition is shown in Figure 1.


Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.

Hu X, Wang C, Wang P - Front Microbiol (2015)

Optimization of medium composition for biosurfactant production using the analytical hierarchy process.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585178&req=5

Figure 1: Optimization of medium composition for biosurfactant production using the analytical hierarchy process.
Mentions: In the AHP model, the top level (the goal) is the optimal medium composition (or operating factors). At the second level, there are four criteria: the biomass (OD600), the protein concentration, the surface tension, and the diameter of clear zone. At the third level, there are n alternatives that must be compared and assessed. An example of the AHP model for a case study of medium composition is shown in Figure 1.

Bottom Line: The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate.Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm.Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences Yantai, China.

ABSTRACT
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.

No MeSH data available.


Related in: MedlinePlus