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Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.).

Zhang L, Zhao HK, Dong QL, Zhang YY, Wang YM, Li HY, Xing GJ, Li QY, Dong YS - Front Plant Sci (2015)

Bottom Line: Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity.The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress.These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.

View Article: PubMed Central - PubMed

Affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences Changchun, China.

ABSTRACT
Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in soybeans (Glycine max L.). In this study, an investigation of the soybean genome revealed 61 putative HSP70 genes, which were evaluated. These genes were classified into eight sub-families, denoted I-VIII, based on a phylogenetic analysis. In each sub-family, the constituent parts of the gene structure and motif were relatively conserved. These GmHSP70 genes were distributed unequally on 17 of the 20 chromosomes. The analysis of the expression profiles showed that 53 of the 61 GmHSP70 genes were differentially expressed across the 14 tissues. However, most of the GmHSP70s were differentially expressed in a tissue-specific expression pattern. Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity. The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress. These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.

No MeSH data available.


Related in: MedlinePlus

Quantitative RT-PCR analysis of the GmHSP70 gene expression in soybean leaves in response to drought stress and 42°C heat stress. (A) Expression levels of 61 GmHSP70 family genes under drought stress. (B) Expression levels of 61 GmHSP70 family genes under heat stress. The normalized relative expression level of the 0 h treatment time point was set up as 1. Leaves collected at 0, 3, 6, 12, and 24 h post-drought or at 0, 3, 6, 12, and 24 h post heat stress. Means are calculated from technical triplicate qRT-PCR measurements within three biological replicates. The qRT-PCR data was shown in the Additional File 14.
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Figure 6: Quantitative RT-PCR analysis of the GmHSP70 gene expression in soybean leaves in response to drought stress and 42°C heat stress. (A) Expression levels of 61 GmHSP70 family genes under drought stress. (B) Expression levels of 61 GmHSP70 family genes under heat stress. The normalized relative expression level of the 0 h treatment time point was set up as 1. Leaves collected at 0, 3, 6, 12, and 24 h post-drought or at 0, 3, 6, 12, and 24 h post heat stress. Means are calculated from technical triplicate qRT-PCR measurements within three biological replicates. The qRT-PCR data was shown in the Additional File 14.

Mentions: In order to gain more insight into the roles of soybean HSP70 genes in heat and drought tolerance, the expression profiles of 61 soybean HSP70 genes in response to drought (0, 3, 6, 12, 24 h) and heat (0, 3, 6, 12, 24 h) stresses were reanalyzed by qRT-PCR. These genes expressed diversely under both stresses (Figures 6A,B). For the drought treatment (15% PEG), these genes could be divided into four clusters. Cluster 1 contains 31 (50.8%) members of detectable GmHSP70 genes, which were widely upregulated after 6 h by drought treatment, and up to the highest expression level at 12 h, then downregulated at 24 h after drought treatment. Cluster 2 mainly consists of 13 (21.3%) genes, which were widely downregulated by drought treatment. Cluster 3 has 6 (9.8%) members, which showed a weakly upregulated at 6 or 12 h, but downregulated at 24 h after drought treatment. Cluster 4 contains 11 (18%) genes, which were mainly upregulated with the increased level of drought treatment after 24 h, and the Glyma02g36700 in particular was highly induced at 3 h after drought treatment. Overall, 30 GmHSP70 genes were up-regulated from two-fold to 222-fold after drought treatment relative to the control, and the other GmHSP70 genes were down-regulated (<0.5-fold) at some stage or showed no changes during the drought stress treatment (Additional File 14).


Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.).

Zhang L, Zhao HK, Dong QL, Zhang YY, Wang YM, Li HY, Xing GJ, Li QY, Dong YS - Front Plant Sci (2015)

Quantitative RT-PCR analysis of the GmHSP70 gene expression in soybean leaves in response to drought stress and 42°C heat stress. (A) Expression levels of 61 GmHSP70 family genes under drought stress. (B) Expression levels of 61 GmHSP70 family genes under heat stress. The normalized relative expression level of the 0 h treatment time point was set up as 1. Leaves collected at 0, 3, 6, 12, and 24 h post-drought or at 0, 3, 6, 12, and 24 h post heat stress. Means are calculated from technical triplicate qRT-PCR measurements within three biological replicates. The qRT-PCR data was shown in the Additional File 14.
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Figure 6: Quantitative RT-PCR analysis of the GmHSP70 gene expression in soybean leaves in response to drought stress and 42°C heat stress. (A) Expression levels of 61 GmHSP70 family genes under drought stress. (B) Expression levels of 61 GmHSP70 family genes under heat stress. The normalized relative expression level of the 0 h treatment time point was set up as 1. Leaves collected at 0, 3, 6, 12, and 24 h post-drought or at 0, 3, 6, 12, and 24 h post heat stress. Means are calculated from technical triplicate qRT-PCR measurements within three biological replicates. The qRT-PCR data was shown in the Additional File 14.
Mentions: In order to gain more insight into the roles of soybean HSP70 genes in heat and drought tolerance, the expression profiles of 61 soybean HSP70 genes in response to drought (0, 3, 6, 12, 24 h) and heat (0, 3, 6, 12, 24 h) stresses were reanalyzed by qRT-PCR. These genes expressed diversely under both stresses (Figures 6A,B). For the drought treatment (15% PEG), these genes could be divided into four clusters. Cluster 1 contains 31 (50.8%) members of detectable GmHSP70 genes, which were widely upregulated after 6 h by drought treatment, and up to the highest expression level at 12 h, then downregulated at 24 h after drought treatment. Cluster 2 mainly consists of 13 (21.3%) genes, which were widely downregulated by drought treatment. Cluster 3 has 6 (9.8%) members, which showed a weakly upregulated at 6 or 12 h, but downregulated at 24 h after drought treatment. Cluster 4 contains 11 (18%) genes, which were mainly upregulated with the increased level of drought treatment after 24 h, and the Glyma02g36700 in particular was highly induced at 3 h after drought treatment. Overall, 30 GmHSP70 genes were up-regulated from two-fold to 222-fold after drought treatment relative to the control, and the other GmHSP70 genes were down-regulated (<0.5-fold) at some stage or showed no changes during the drought stress treatment (Additional File 14).

Bottom Line: Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity.The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress.These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.

View Article: PubMed Central - PubMed

Affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences Changchun, China.

ABSTRACT
Heat shock proteins (HSPs) perform a fundamental role in protecting plants against abiotic stresses. Previous studies have made great efforts in the functional analysis of individual family members, but there has not yet been an overall analysis or expression profiling of the HSP70 gene family in soybeans (Glycine max L.). In this study, an investigation of the soybean genome revealed 61 putative HSP70 genes, which were evaluated. These genes were classified into eight sub-families, denoted I-VIII, based on a phylogenetic analysis. In each sub-family, the constituent parts of the gene structure and motif were relatively conserved. These GmHSP70 genes were distributed unequally on 17 of the 20 chromosomes. The analysis of the expression profiles showed that 53 of the 61 GmHSP70 genes were differentially expressed across the 14 tissues. However, most of the GmHSP70s were differentially expressed in a tissue-specific expression pattern. Furthermore, the expression of some of the duplicate genes was partially redundant, while others showed functional diversity. The quantitative real-time PCR (qRT-PCR) analysis of the 61 soybean HSP70 genes confirmed their stress-inducible expression patterns under both drought and heat stress. These findings provide a thorough overview of the evolution and modification of the GmHSP70 gene family, which will help to determine the functional characteristics of the HSP70 genes in soybean growth and development.

No MeSH data available.


Related in: MedlinePlus