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Interaction of fibrinogen and muramidase-released protein promotes the development of Streptococcus suis meningitis.

Wang J, Kong D, Zhang S, Jiang H, Zheng Y, Zang Y, Hao H, Jiang Y - Front Microbiol (2015)

Bottom Line: Muramidase-released protein (MRP) is as an important virulence marker of Streptococcus suis (S. suis) serotype 2.In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3).Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB).

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences , Beijing, China ; Urumqi Ethnic Cadres' College , Urumqi, China.

ABSTRACT
Muramidase-released protein (MRP) is as an important virulence marker of Streptococcus suis (S. suis) serotype 2. Our previous works have shown that MRP can bind human fibrinogen (hFg); however, the function of this interaction in S. suis meningitis is not known. In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3). Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB). In the mouse meningitis model, wild type S. suis caused higher bacterial loads in the brain and more severe histopathological signs of meningitis than the mrp mutant at day 3 post-infection. Western blot analysis and immunofluorescence observations reveal that the MRP-hFg interaction can destroy the cell adherens junction protein p120-catenin of hCMEC/D3. These results indicate that the MRP-hFg interaction is important in the development of S. suis meningitis.

No MeSH data available.


Related in: MedlinePlus

S. suis 05ZYH33 infection increases the blood–brain barrier permeability of mice. (A) Representative images of whole brains stained by Evans blue dye (days 1, 2, 3). (B) Evans blue extravasations in brains at day 3 post-infection, data are expressed as mean ± SEM, n = 6 in each group, *P < 0.05.
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Figure 3: S. suis 05ZYH33 infection increases the blood–brain barrier permeability of mice. (A) Representative images of whole brains stained by Evans blue dye (days 1, 2, 3). (B) Evans blue extravasations in brains at day 3 post-infection, data are expressed as mean ± SEM, n = 6 in each group, *P < 0.05.

Mentions: To evaluate the BBB permeability in vivo, we administered Evans Blue, a dye that is normally excluded from the CNS, to S. suis 05ZYH33 and Δmrp mutant infected mice through an intraperitoneal injection at days 1, 2, and 3 post-infection. Macroscopic inspection of the brain showed more visible penetration of Evans Blue in S. suis 05ZYH33 infected mice than that in Δmrp mutant infected mice at day 3 post-infection (Figure 3A). Quantitative evaluation of extravasated Evans Blue also revealed higher levels in the brain lysates from S. suis 05ZYH33 infected mice than that from Δmrp mutant infected mice at day 3 post-infection (Figure 3B). These data show that MRP might modulate the BBB permeability during the development of S. suis meningitis.


Interaction of fibrinogen and muramidase-released protein promotes the development of Streptococcus suis meningitis.

Wang J, Kong D, Zhang S, Jiang H, Zheng Y, Zang Y, Hao H, Jiang Y - Front Microbiol (2015)

S. suis 05ZYH33 infection increases the blood–brain barrier permeability of mice. (A) Representative images of whole brains stained by Evans blue dye (days 1, 2, 3). (B) Evans blue extravasations in brains at day 3 post-infection, data are expressed as mean ± SEM, n = 6 in each group, *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585153&req=5

Figure 3: S. suis 05ZYH33 infection increases the blood–brain barrier permeability of mice. (A) Representative images of whole brains stained by Evans blue dye (days 1, 2, 3). (B) Evans blue extravasations in brains at day 3 post-infection, data are expressed as mean ± SEM, n = 6 in each group, *P < 0.05.
Mentions: To evaluate the BBB permeability in vivo, we administered Evans Blue, a dye that is normally excluded from the CNS, to S. suis 05ZYH33 and Δmrp mutant infected mice through an intraperitoneal injection at days 1, 2, and 3 post-infection. Macroscopic inspection of the brain showed more visible penetration of Evans Blue in S. suis 05ZYH33 infected mice than that in Δmrp mutant infected mice at day 3 post-infection (Figure 3A). Quantitative evaluation of extravasated Evans Blue also revealed higher levels in the brain lysates from S. suis 05ZYH33 infected mice than that from Δmrp mutant infected mice at day 3 post-infection (Figure 3B). These data show that MRP might modulate the BBB permeability during the development of S. suis meningitis.

Bottom Line: Muramidase-released protein (MRP) is as an important virulence marker of Streptococcus suis (S. suis) serotype 2.In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3).Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB).

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences , Beijing, China ; Urumqi Ethnic Cadres' College , Urumqi, China.

ABSTRACT
Muramidase-released protein (MRP) is as an important virulence marker of Streptococcus suis (S. suis) serotype 2. Our previous works have shown that MRP can bind human fibrinogen (hFg); however, the function of this interaction in S. suis meningitis is not known. In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3). Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB). In the mouse meningitis model, wild type S. suis caused higher bacterial loads in the brain and more severe histopathological signs of meningitis than the mrp mutant at day 3 post-infection. Western blot analysis and immunofluorescence observations reveal that the MRP-hFg interaction can destroy the cell adherens junction protein p120-catenin of hCMEC/D3. These results indicate that the MRP-hFg interaction is important in the development of S. suis meningitis.

No MeSH data available.


Related in: MedlinePlus