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Metabolite profiling and transcript analysis reveal specificities in the response of a berry derived cell culture to abiotic stresses.

Ayenew B, Degu A, Manela N, Perl A, Shamir MO, Fait A - Front Plant Sci (2015)

Bottom Line: As climate changes, there is a need to understand the expected effects on viticulture.The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway.HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms.

View Article: PubMed Central - PubMed

Affiliation: The Albert Katz International School for Desert Studies, Ben-Gurion University of the Negev Beer-Sheva, Israel ; The French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede Boqer, Israel.

ABSTRACT
As climate changes, there is a need to understand the expected effects on viticulture. In nature, stresses exist in a combined manner, hampering the elucidation of the effect of individual cues on grape berry metabolism. Cell suspension culture originated from pea-size Gamy Red grape berry was used to harness metabolic response to high light (HL; 2500 μmol m(-2)s(-1)), high temperature (HT; 40°C) and their combination in comparison to 25°C and 100 μmol m(-2)s(-1) under controlled condition. When LC-MS and GC-MS based metabolite profiling was implemented and integrated with targeted RT-qPCR transcript analysis specific responses were observed to the different cues. HL enhanced polyphenol metabolism while HT and its combination with HL induced amino acid and organic acid metabolism with additional effect on polyphenols. The trend of increment in TCA cycle genes like ATCs, ACo1, and IDH in the combined treatment might support the observed increment in organic acids, GABA shunt, and their derivatives. The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway. In the polyphenol metabolism, a differential pattern of expression of flavonoid 3',5' hydroxylase and flavonoid 3' hydroxylase was observed under high light (HL) and combined cues which were accompanied by characteristic metabolite profiles. HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms. Transcription factors regulating anthocyanin metabolism and their methylation, MYB, OMT, UFGT, and DFR, were expressed differentially among the treatments, overall in agreement with the metabolite profiles. Taken together these data provide insights into the coordination of central and secondary metabolism in relation to multiple abiotic stresses.

No MeSH data available.


Related in: MedlinePlus

Fold change transcript levels of enzymes and transcription factors mediating phenylpropanoid pathway from respective time point control. Fold change values between zero and one (below the dashed line) indicates lower transcrip level compared to the control. ACC, Acetyl-CoA carboxylase 1-like; ANR, Anthocyanidin reductase; C3H, Coumarate 3-hydroxylase; CHS, Chalcone synthase; DFR, Dihydroflavonol 4-reductase; F3′H:flavonoid 3′ hydroxylase; F3′5′H: flavonoid 3′,5′ hydroxylase; MYB, Myb-related transcription factor; OMT, O-methyltransferase; PAL, Phenylalanine ammonia lyase; STS, Stilbene synthase; UFGT, UDP flavonoid 3-O-glucosyltransferase; n = 3, mean values ± SE.
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Figure 5: Fold change transcript levels of enzymes and transcription factors mediating phenylpropanoid pathway from respective time point control. Fold change values between zero and one (below the dashed line) indicates lower transcrip level compared to the control. ACC, Acetyl-CoA carboxylase 1-like; ANR, Anthocyanidin reductase; C3H, Coumarate 3-hydroxylase; CHS, Chalcone synthase; DFR, Dihydroflavonol 4-reductase; F3′H:flavonoid 3′ hydroxylase; F3′5′H: flavonoid 3′,5′ hydroxylase; MYB, Myb-related transcription factor; OMT, O-methyltransferase; PAL, Phenylalanine ammonia lyase; STS, Stilbene synthase; UFGT, UDP flavonoid 3-O-glucosyltransferase; n = 3, mean values ± SE.

Mentions: Transcripts responsible for mediating phenylpropanoid pathway were expressed differently between treatments (Figure 5; Supplementary Table S4) and associations between the level of expression of specific genes and related metabolites were observed, particularly in relatively downstream biochemical reactions.


Metabolite profiling and transcript analysis reveal specificities in the response of a berry derived cell culture to abiotic stresses.

Ayenew B, Degu A, Manela N, Perl A, Shamir MO, Fait A - Front Plant Sci (2015)

Fold change transcript levels of enzymes and transcription factors mediating phenylpropanoid pathway from respective time point control. Fold change values between zero and one (below the dashed line) indicates lower transcrip level compared to the control. ACC, Acetyl-CoA carboxylase 1-like; ANR, Anthocyanidin reductase; C3H, Coumarate 3-hydroxylase; CHS, Chalcone synthase; DFR, Dihydroflavonol 4-reductase; F3′H:flavonoid 3′ hydroxylase; F3′5′H: flavonoid 3′,5′ hydroxylase; MYB, Myb-related transcription factor; OMT, O-methyltransferase; PAL, Phenylalanine ammonia lyase; STS, Stilbene synthase; UFGT, UDP flavonoid 3-O-glucosyltransferase; n = 3, mean values ± SE.
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Related In: Results  -  Collection

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Figure 5: Fold change transcript levels of enzymes and transcription factors mediating phenylpropanoid pathway from respective time point control. Fold change values between zero and one (below the dashed line) indicates lower transcrip level compared to the control. ACC, Acetyl-CoA carboxylase 1-like; ANR, Anthocyanidin reductase; C3H, Coumarate 3-hydroxylase; CHS, Chalcone synthase; DFR, Dihydroflavonol 4-reductase; F3′H:flavonoid 3′ hydroxylase; F3′5′H: flavonoid 3′,5′ hydroxylase; MYB, Myb-related transcription factor; OMT, O-methyltransferase; PAL, Phenylalanine ammonia lyase; STS, Stilbene synthase; UFGT, UDP flavonoid 3-O-glucosyltransferase; n = 3, mean values ± SE.
Mentions: Transcripts responsible for mediating phenylpropanoid pathway were expressed differently between treatments (Figure 5; Supplementary Table S4) and associations between the level of expression of specific genes and related metabolites were observed, particularly in relatively downstream biochemical reactions.

Bottom Line: As climate changes, there is a need to understand the expected effects on viticulture.The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway.HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms.

View Article: PubMed Central - PubMed

Affiliation: The Albert Katz International School for Desert Studies, Ben-Gurion University of the Negev Beer-Sheva, Israel ; The French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede Boqer, Israel.

ABSTRACT
As climate changes, there is a need to understand the expected effects on viticulture. In nature, stresses exist in a combined manner, hampering the elucidation of the effect of individual cues on grape berry metabolism. Cell suspension culture originated from pea-size Gamy Red grape berry was used to harness metabolic response to high light (HL; 2500 μmol m(-2)s(-1)), high temperature (HT; 40°C) and their combination in comparison to 25°C and 100 μmol m(-2)s(-1) under controlled condition. When LC-MS and GC-MS based metabolite profiling was implemented and integrated with targeted RT-qPCR transcript analysis specific responses were observed to the different cues. HL enhanced polyphenol metabolism while HT and its combination with HL induced amino acid and organic acid metabolism with additional effect on polyphenols. The trend of increment in TCA cycle genes like ATCs, ACo1, and IDH in the combined treatment might support the observed increment in organic acids, GABA shunt, and their derivatives. The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway. In the polyphenol metabolism, a differential pattern of expression of flavonoid 3',5' hydroxylase and flavonoid 3' hydroxylase was observed under high light (HL) and combined cues which were accompanied by characteristic metabolite profiles. HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms. Transcription factors regulating anthocyanin metabolism and their methylation, MYB, OMT, UFGT, and DFR, were expressed differentially among the treatments, overall in agreement with the metabolite profiles. Taken together these data provide insights into the coordination of central and secondary metabolism in relation to multiple abiotic stresses.

No MeSH data available.


Related in: MedlinePlus