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Metabolite profiling and transcript analysis reveal specificities in the response of a berry derived cell culture to abiotic stresses.

Ayenew B, Degu A, Manela N, Perl A, Shamir MO, Fait A - Front Plant Sci (2015)

Bottom Line: As climate changes, there is a need to understand the expected effects on viticulture.The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway.HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms.

View Article: PubMed Central - PubMed

Affiliation: The Albert Katz International School for Desert Studies, Ben-Gurion University of the Negev Beer-Sheva, Israel ; The French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede Boqer, Israel.

ABSTRACT
As climate changes, there is a need to understand the expected effects on viticulture. In nature, stresses exist in a combined manner, hampering the elucidation of the effect of individual cues on grape berry metabolism. Cell suspension culture originated from pea-size Gamy Red grape berry was used to harness metabolic response to high light (HL; 2500 μmol m(-2)s(-1)), high temperature (HT; 40°C) and their combination in comparison to 25°C and 100 μmol m(-2)s(-1) under controlled condition. When LC-MS and GC-MS based metabolite profiling was implemented and integrated with targeted RT-qPCR transcript analysis specific responses were observed to the different cues. HL enhanced polyphenol metabolism while HT and its combination with HL induced amino acid and organic acid metabolism with additional effect on polyphenols. The trend of increment in TCA cycle genes like ATCs, ACo1, and IDH in the combined treatment might support the observed increment in organic acids, GABA shunt, and their derivatives. The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway. In the polyphenol metabolism, a differential pattern of expression of flavonoid 3',5' hydroxylase and flavonoid 3' hydroxylase was observed under high light (HL) and combined cues which were accompanied by characteristic metabolite profiles. HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms. Transcription factors regulating anthocyanin metabolism and their methylation, MYB, OMT, UFGT, and DFR, were expressed differentially among the treatments, overall in agreement with the metabolite profiles. Taken together these data provide insights into the coordination of central and secondary metabolism in relation to multiple abiotic stresses.

No MeSH data available.


Related in: MedlinePlus

Schematic representations of primary metabolites in fold change from respective time point control in Log2 values. Different colors represent levels of metabolite fold change where red is increasing and blue is decreasing. Mean values are presented (n = 4). Each row (upper row = HL treatment, middle row = combined HL with high temperature, lower row = HT) shows fold change from respective time point control while each column is different time points after treatment application (4, 8, 12, and 24 h).
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Figure 3: Schematic representations of primary metabolites in fold change from respective time point control in Log2 values. Different colors represent levels of metabolite fold change where red is increasing and blue is decreasing. Mean values are presented (n = 4). Each row (upper row = HL treatment, middle row = combined HL with high temperature, lower row = HT) shows fold change from respective time point control while each column is different time points after treatment application (4, 8, 12, and 24 h).

Mentions: A communal and rapid decrease in sugar reserves, pentose phosphate pathway (PPP) and glycolysis intermediates were observed during HL and combined stresses (Figure 3; Supplementary Table S3). Apart from these, HL stimulated the accumulation of stress related sugars such as raffinose and galactose and a significant reduction in amino acid content during the experimental period (Figure 3; Supplementary Table S3). TCA cycle intermediates such as maleate, aspartate, and TCA cycle associated GABA shunt were not affected significantly under HL stress showing a general trend of decreasing relative content over time (Figure 3; Supplementary Table S3).


Metabolite profiling and transcript analysis reveal specificities in the response of a berry derived cell culture to abiotic stresses.

Ayenew B, Degu A, Manela N, Perl A, Shamir MO, Fait A - Front Plant Sci (2015)

Schematic representations of primary metabolites in fold change from respective time point control in Log2 values. Different colors represent levels of metabolite fold change where red is increasing and blue is decreasing. Mean values are presented (n = 4). Each row (upper row = HL treatment, middle row = combined HL with high temperature, lower row = HT) shows fold change from respective time point control while each column is different time points after treatment application (4, 8, 12, and 24 h).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585150&req=5

Figure 3: Schematic representations of primary metabolites in fold change from respective time point control in Log2 values. Different colors represent levels of metabolite fold change where red is increasing and blue is decreasing. Mean values are presented (n = 4). Each row (upper row = HL treatment, middle row = combined HL with high temperature, lower row = HT) shows fold change from respective time point control while each column is different time points after treatment application (4, 8, 12, and 24 h).
Mentions: A communal and rapid decrease in sugar reserves, pentose phosphate pathway (PPP) and glycolysis intermediates were observed during HL and combined stresses (Figure 3; Supplementary Table S3). Apart from these, HL stimulated the accumulation of stress related sugars such as raffinose and galactose and a significant reduction in amino acid content during the experimental period (Figure 3; Supplementary Table S3). TCA cycle intermediates such as maleate, aspartate, and TCA cycle associated GABA shunt were not affected significantly under HL stress showing a general trend of decreasing relative content over time (Figure 3; Supplementary Table S3).

Bottom Line: As climate changes, there is a need to understand the expected effects on viticulture.The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway.HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms.

View Article: PubMed Central - PubMed

Affiliation: The Albert Katz International School for Desert Studies, Ben-Gurion University of the Negev Beer-Sheva, Israel ; The French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede Boqer, Israel.

ABSTRACT
As climate changes, there is a need to understand the expected effects on viticulture. In nature, stresses exist in a combined manner, hampering the elucidation of the effect of individual cues on grape berry metabolism. Cell suspension culture originated from pea-size Gamy Red grape berry was used to harness metabolic response to high light (HL; 2500 μmol m(-2)s(-1)), high temperature (HT; 40°C) and their combination in comparison to 25°C and 100 μmol m(-2)s(-1) under controlled condition. When LC-MS and GC-MS based metabolite profiling was implemented and integrated with targeted RT-qPCR transcript analysis specific responses were observed to the different cues. HL enhanced polyphenol metabolism while HT and its combination with HL induced amino acid and organic acid metabolism with additional effect on polyphenols. The trend of increment in TCA cycle genes like ATCs, ACo1, and IDH in the combined treatment might support the observed increment in organic acids, GABA shunt, and their derivatives. The apparent phenylalanine reduction with polyphenol increment under HL suggests enhanced fueling of the precursor toward the downstream phenylpropanoid pathway. In the polyphenol metabolism, a differential pattern of expression of flavonoid 3',5' hydroxylase and flavonoid 3' hydroxylase was observed under high light (HL) and combined cues which were accompanied by characteristic metabolite profiles. HT decreased glycosylated cyanidin and peonidin forms while the combined cues increased acetylated and coumarylated peonidin forms. Transcription factors regulating anthocyanin metabolism and their methylation, MYB, OMT, UFGT, and DFR, were expressed differentially among the treatments, overall in agreement with the metabolite profiles. Taken together these data provide insights into the coordination of central and secondary metabolism in relation to multiple abiotic stresses.

No MeSH data available.


Related in: MedlinePlus