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Analysis of the transgenerational iron deficiency stress memory in Arabidopsis thaliana plants.

Murgia I, Giacometti S, Balestrazzi A, Paparella S, Pagliano C, Morandini P - Front Plant Sci (2015)

Bottom Line: However, SHR frequency, DNA strand break events, and TFIIS-like gene expression do not increase further when plants are grown for more than one generation under the same stress, and furthermore, they decrease back to control values within two succeeding generations grown under control conditions, regardless of the Fe deficiency stress history of the mother plants.Lastly, plants grown for multiple generations under Fe deficiency produce seeds with greater longevity: however, this trait is not inherited in offspring generations unexposed to stress.These findings suggest the existence of multiple-step control of mechanisms to prevent a genuine and stable transgenerational transmission of Fe deficiency stress memory, with the tightest control on DNA integrity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences, University of Milano Milano, Italy.

ABSTRACT
We investigated the existence of the transgenerational memory of iron (Fe) deficiency stress, in Arabidopsis thaliana. Plants were grown under Fe deficiency/sufficiency, and so were their offspring. The frequency of somatic homologous recombination (SHR) events, of DNA strand breaks as well as the expression of the transcription elongation factor TFIIS-like gene increase when plants are grown under Fe deficiency. However, SHR frequency, DNA strand break events, and TFIIS-like gene expression do not increase further when plants are grown for more than one generation under the same stress, and furthermore, they decrease back to control values within two succeeding generations grown under control conditions, regardless of the Fe deficiency stress history of the mother plants. Seedlings produced from plants grown under Fe deficiency evolve more oxygen than control seedlings, when grown under Fe sufficiency: however, this trait is not associated with any change in the protein profile of the photosynthetic apparatus and is not transmitted to more than one generation. Lastly, plants grown for multiple generations under Fe deficiency produce seeds with greater longevity: however, this trait is not inherited in offspring generations unexposed to stress. These findings suggest the existence of multiple-step control of mechanisms to prevent a genuine and stable transgenerational transmission of Fe deficiency stress memory, with the tightest control on DNA integrity.

No MeSH data available.


Related in: MedlinePlus

Production of the Arabidopsis thaliana plants with generational exposure to Fe deficiency.A. thaliana wt ecotype Col or A. thaliana SHR-trap 1445 line (background Col) were used as control c0 generation (c0 seeds). c0 seeds grown in control soil produced seeds that are still named c0; c0 seeds grown in either pH 7.7 or pH 8.4 soil produced pH 7.7 s1 or pH 8.4 s1 seeds, respectively. pH 7.7 s1 seeds grown in either pH 7.7 or control soil produced pH 7.7 s1 pH 7.7 s2 or pH 7.7 s1 c2 seeds, respectively. pH 8.4 s1 seeds grown in either pH 8.4 or control soil produced pH 8.4 s1 pH 8.4 s2 or pH 8.4 s1 c2 seeds, respectively.
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Figure 1: Production of the Arabidopsis thaliana plants with generational exposure to Fe deficiency.A. thaliana wt ecotype Col or A. thaliana SHR-trap 1445 line (background Col) were used as control c0 generation (c0 seeds). c0 seeds grown in control soil produced seeds that are still named c0; c0 seeds grown in either pH 7.7 or pH 8.4 soil produced pH 7.7 s1 or pH 8.4 s1 seeds, respectively. pH 7.7 s1 seeds grown in either pH 7.7 or control soil produced pH 7.7 s1 pH 7.7 s2 or pH 7.7 s1 c2 seeds, respectively. pH 8.4 s1 seeds grown in either pH 8.4 or control soil produced pH 8.4 s1 pH 8.4 s2 or pH 8.4 s1 c2 seeds, respectively.

Mentions: Col pH 7.7 s1 and pH 8.4 s1 plants, when grown either in control, pH 7.7 (Supplementary Figures S1A,B) or pH 8.4 soil (Supplementary Figure S1C), show higher biomass production than corresponding c0 plants grown in the same conditions (Supplementary Figure S1), thus suggesting the existence of a memory of Fe deficiency stress in the offspring. To confirm the existence of a genuine transgenerational memory, the SHR-trap line 1445 (control generation c0), was grown in either of two alkaline soils, at either pH 7.7 or 8.4, with production of pH 7.7 s1 and pH 8.4 s1 generation seeds, respectively (Figure 1).


Analysis of the transgenerational iron deficiency stress memory in Arabidopsis thaliana plants.

Murgia I, Giacometti S, Balestrazzi A, Paparella S, Pagliano C, Morandini P - Front Plant Sci (2015)

Production of the Arabidopsis thaliana plants with generational exposure to Fe deficiency.A. thaliana wt ecotype Col or A. thaliana SHR-trap 1445 line (background Col) were used as control c0 generation (c0 seeds). c0 seeds grown in control soil produced seeds that are still named c0; c0 seeds grown in either pH 7.7 or pH 8.4 soil produced pH 7.7 s1 or pH 8.4 s1 seeds, respectively. pH 7.7 s1 seeds grown in either pH 7.7 or control soil produced pH 7.7 s1 pH 7.7 s2 or pH 7.7 s1 c2 seeds, respectively. pH 8.4 s1 seeds grown in either pH 8.4 or control soil produced pH 8.4 s1 pH 8.4 s2 or pH 8.4 s1 c2 seeds, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585125&req=5

Figure 1: Production of the Arabidopsis thaliana plants with generational exposure to Fe deficiency.A. thaliana wt ecotype Col or A. thaliana SHR-trap 1445 line (background Col) were used as control c0 generation (c0 seeds). c0 seeds grown in control soil produced seeds that are still named c0; c0 seeds grown in either pH 7.7 or pH 8.4 soil produced pH 7.7 s1 or pH 8.4 s1 seeds, respectively. pH 7.7 s1 seeds grown in either pH 7.7 or control soil produced pH 7.7 s1 pH 7.7 s2 or pH 7.7 s1 c2 seeds, respectively. pH 8.4 s1 seeds grown in either pH 8.4 or control soil produced pH 8.4 s1 pH 8.4 s2 or pH 8.4 s1 c2 seeds, respectively.
Mentions: Col pH 7.7 s1 and pH 8.4 s1 plants, when grown either in control, pH 7.7 (Supplementary Figures S1A,B) or pH 8.4 soil (Supplementary Figure S1C), show higher biomass production than corresponding c0 plants grown in the same conditions (Supplementary Figure S1), thus suggesting the existence of a memory of Fe deficiency stress in the offspring. To confirm the existence of a genuine transgenerational memory, the SHR-trap line 1445 (control generation c0), was grown in either of two alkaline soils, at either pH 7.7 or 8.4, with production of pH 7.7 s1 and pH 8.4 s1 generation seeds, respectively (Figure 1).

Bottom Line: However, SHR frequency, DNA strand break events, and TFIIS-like gene expression do not increase further when plants are grown for more than one generation under the same stress, and furthermore, they decrease back to control values within two succeeding generations grown under control conditions, regardless of the Fe deficiency stress history of the mother plants.Lastly, plants grown for multiple generations under Fe deficiency produce seeds with greater longevity: however, this trait is not inherited in offspring generations unexposed to stress.These findings suggest the existence of multiple-step control of mechanisms to prevent a genuine and stable transgenerational transmission of Fe deficiency stress memory, with the tightest control on DNA integrity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences, University of Milano Milano, Italy.

ABSTRACT
We investigated the existence of the transgenerational memory of iron (Fe) deficiency stress, in Arabidopsis thaliana. Plants were grown under Fe deficiency/sufficiency, and so were their offspring. The frequency of somatic homologous recombination (SHR) events, of DNA strand breaks as well as the expression of the transcription elongation factor TFIIS-like gene increase when plants are grown under Fe deficiency. However, SHR frequency, DNA strand break events, and TFIIS-like gene expression do not increase further when plants are grown for more than one generation under the same stress, and furthermore, they decrease back to control values within two succeeding generations grown under control conditions, regardless of the Fe deficiency stress history of the mother plants. Seedlings produced from plants grown under Fe deficiency evolve more oxygen than control seedlings, when grown under Fe sufficiency: however, this trait is not associated with any change in the protein profile of the photosynthetic apparatus and is not transmitted to more than one generation. Lastly, plants grown for multiple generations under Fe deficiency produce seeds with greater longevity: however, this trait is not inherited in offspring generations unexposed to stress. These findings suggest the existence of multiple-step control of mechanisms to prevent a genuine and stable transgenerational transmission of Fe deficiency stress memory, with the tightest control on DNA integrity.

No MeSH data available.


Related in: MedlinePlus