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A Populus TIR1 gene family survey reveals differential expression patterns and responses to 1-naphthaleneacetic acid and stress treatments.

Shu W, Liu Y, Guo Y, Zhou H, Zhang J, Zhao S, Lu M - Front Plant Sci (2015)

Bottom Line: Interestingly, PtrFBL1 and 7 were expressed mainly in vascular and cambial tissues, respectively, indicating their potential but different roles in wood formation.Finally, over-expression studies indicated a role of FBL1 in poplar stem growth and response to drought stress.Collectively, these observations lay the foundation for further investigations into the potential roles of PtrFBL genes in tree growth and development.

View Article: PubMed Central - PubMed

Affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China ; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.

ABSTRACT
The plant hormone auxin is a central regulator of plant growth. TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFB) is a component of the E3 ubiquitin ligase complex SCF(TIR1/AFB) and acts as an auxin co-receptor for nuclear auxin signaling. The SCF(TIR1/AFB)-proteasome machinery plays a central regulatory role in development-related gene transcription. Populus trichocarpa, as a model tree, has a unique fast-growth trait to which auxin signaling may contribute. However, no systematic analyses of the genome organization, gene structure, and expression of TIR1-like genes have been undertaken in this woody model plant. In this study, we identified a total of eight TIR1 genes in the Populus genome that are phylogenetically clustered into four subgroups, PtrFBL1/PtrFBL2, PtrFBL3/PtrFBL4, PtrFBL5/PtrFBL6, and PtrFBL7/PtrFBL8, representing four paralogous pairs. In addition, the gene structure and motif composition were relatively conserved in each paralogous pair and all of the PtrFBL members were localized in the nucleus. Different sets of PtrFBLs were strongly expressed in the leaves, stems, roots, cambial zones, and immature xylem of Populus. Interestingly, PtrFBL1 and 7 were expressed mainly in vascular and cambial tissues, respectively, indicating their potential but different roles in wood formation. Furthermore, Populus FBLs responded differentially upon exposure to various stresses. Finally, over-expression studies indicated a role of FBL1 in poplar stem growth and response to drought stress. Collectively, these observations lay the foundation for further investigations into the potential roles of PtrFBL genes in tree growth and development.

No MeSH data available.


Related in: MedlinePlus

PtrFBL protein localization (A–H) and semi-quantitative reverse-transcriptional PCR analysis of the PtrFBL expression (I). (A) PtrFBL1, (B) PtrFBL2, (C) PtrFBL3, (D) PtrFBL4, (E) PtrFBL5, (F) PtrFBL6, (G) PtrFBL7, and (H) PtrFBL8.
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Figure 2: PtrFBL protein localization (A–H) and semi-quantitative reverse-transcriptional PCR analysis of the PtrFBL expression (I). (A) PtrFBL1, (B) PtrFBL2, (C) PtrFBL3, (D) PtrFBL4, (E) PtrFBL5, (F) PtrFBL6, (G) PtrFBL7, and (H) PtrFBL8.

Mentions: All the PtrFBLs were predicted to be localized in the nucleus using WoLF PSORT (Table S2). To confirm this, these proteins, fused with YFP at the C-terminus, were transiently expressed in tobacco leaf epidermal cells, and the fusion proteins were observed in the nucleus as indicated by co-localization with DAPI signals (Figures 2A–H). This was consistent with the nuclear localizations of TIR1/AFB proteins in Arabidopsis (Dharmasiri et al., 2005a; Tan et al., 2007). The conserved organelle localization of PtrFBLs implied their conserved functions, such as the regulation of the cellular auxin response.


A Populus TIR1 gene family survey reveals differential expression patterns and responses to 1-naphthaleneacetic acid and stress treatments.

Shu W, Liu Y, Guo Y, Zhou H, Zhang J, Zhao S, Lu M - Front Plant Sci (2015)

PtrFBL protein localization (A–H) and semi-quantitative reverse-transcriptional PCR analysis of the PtrFBL expression (I). (A) PtrFBL1, (B) PtrFBL2, (C) PtrFBL3, (D) PtrFBL4, (E) PtrFBL5, (F) PtrFBL6, (G) PtrFBL7, and (H) PtrFBL8.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585115&req=5

Figure 2: PtrFBL protein localization (A–H) and semi-quantitative reverse-transcriptional PCR analysis of the PtrFBL expression (I). (A) PtrFBL1, (B) PtrFBL2, (C) PtrFBL3, (D) PtrFBL4, (E) PtrFBL5, (F) PtrFBL6, (G) PtrFBL7, and (H) PtrFBL8.
Mentions: All the PtrFBLs were predicted to be localized in the nucleus using WoLF PSORT (Table S2). To confirm this, these proteins, fused with YFP at the C-terminus, were transiently expressed in tobacco leaf epidermal cells, and the fusion proteins were observed in the nucleus as indicated by co-localization with DAPI signals (Figures 2A–H). This was consistent with the nuclear localizations of TIR1/AFB proteins in Arabidopsis (Dharmasiri et al., 2005a; Tan et al., 2007). The conserved organelle localization of PtrFBLs implied their conserved functions, such as the regulation of the cellular auxin response.

Bottom Line: Interestingly, PtrFBL1 and 7 were expressed mainly in vascular and cambial tissues, respectively, indicating their potential but different roles in wood formation.Finally, over-expression studies indicated a role of FBL1 in poplar stem growth and response to drought stress.Collectively, these observations lay the foundation for further investigations into the potential roles of PtrFBL genes in tree growth and development.

View Article: PubMed Central - PubMed

Affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China ; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.

ABSTRACT
The plant hormone auxin is a central regulator of plant growth. TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFB) is a component of the E3 ubiquitin ligase complex SCF(TIR1/AFB) and acts as an auxin co-receptor for nuclear auxin signaling. The SCF(TIR1/AFB)-proteasome machinery plays a central regulatory role in development-related gene transcription. Populus trichocarpa, as a model tree, has a unique fast-growth trait to which auxin signaling may contribute. However, no systematic analyses of the genome organization, gene structure, and expression of TIR1-like genes have been undertaken in this woody model plant. In this study, we identified a total of eight TIR1 genes in the Populus genome that are phylogenetically clustered into four subgroups, PtrFBL1/PtrFBL2, PtrFBL3/PtrFBL4, PtrFBL5/PtrFBL6, and PtrFBL7/PtrFBL8, representing four paralogous pairs. In addition, the gene structure and motif composition were relatively conserved in each paralogous pair and all of the PtrFBL members were localized in the nucleus. Different sets of PtrFBLs were strongly expressed in the leaves, stems, roots, cambial zones, and immature xylem of Populus. Interestingly, PtrFBL1 and 7 were expressed mainly in vascular and cambial tissues, respectively, indicating their potential but different roles in wood formation. Furthermore, Populus FBLs responded differentially upon exposure to various stresses. Finally, over-expression studies indicated a role of FBL1 in poplar stem growth and response to drought stress. Collectively, these observations lay the foundation for further investigations into the potential roles of PtrFBL genes in tree growth and development.

No MeSH data available.


Related in: MedlinePlus