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Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Histograms depict changes between PDPCs and ASCs mRNA expression of bmp2, Sox 9 and Collagen Type II observed after culturing cells for 14 and 21 days on GEL with chondrogenic differentiating medium. (A) Fold-changes of PDPCs and ASCs seeded on scaffolds with respect to control cultures (i.e., PDPCs and ASCs in tissue culture plates with chondrogenic differentiating medium); (B) Fold changes of PDPCs and ASCs seeded on GEL (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
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Figure 6: Histograms depict changes between PDPCs and ASCs mRNA expression of bmp2, Sox 9 and Collagen Type II observed after culturing cells for 14 and 21 days on GEL with chondrogenic differentiating medium. (A) Fold-changes of PDPCs and ASCs seeded on scaffolds with respect to control cultures (i.e., PDPCs and ASCs in tissue culture plates with chondrogenic differentiating medium); (B) Fold changes of PDPCs and ASCs seeded on GEL (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).

Mentions: As far as cells seeded on GEL scaffold and induced toward a chondrogenic differentiation Bmp2 resulted significantly down regulated in PDPCs, while it remained unchanged in ASCs. Sox9 expression was down regulated in both cell cytotypes at 14 days of culture, whilst it appeared unmodified at 21 days of culture. Results of the comparison of Type II collagen mRNA expression in cells seeded onto the scaffolds with control culture in plastic showed a down regulation of this gene at 14 days that was more marked in ASCs. On the contrary, after 21 days the production of Type II collagen increased for both tested cytotypes (Figure 6A).


Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Histograms depict changes between PDPCs and ASCs mRNA expression of bmp2, Sox 9 and Collagen Type II observed after culturing cells for 14 and 21 days on GEL with chondrogenic differentiating medium. (A) Fold-changes of PDPCs and ASCs seeded on scaffolds with respect to control cultures (i.e., PDPCs and ASCs in tissue culture plates with chondrogenic differentiating medium); (B) Fold changes of PDPCs and ASCs seeded on GEL (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585109&req=5

Figure 6: Histograms depict changes between PDPCs and ASCs mRNA expression of bmp2, Sox 9 and Collagen Type II observed after culturing cells for 14 and 21 days on GEL with chondrogenic differentiating medium. (A) Fold-changes of PDPCs and ASCs seeded on scaffolds with respect to control cultures (i.e., PDPCs and ASCs in tissue culture plates with chondrogenic differentiating medium); (B) Fold changes of PDPCs and ASCs seeded on GEL (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
Mentions: As far as cells seeded on GEL scaffold and induced toward a chondrogenic differentiation Bmp2 resulted significantly down regulated in PDPCs, while it remained unchanged in ASCs. Sox9 expression was down regulated in both cell cytotypes at 14 days of culture, whilst it appeared unmodified at 21 days of culture. Results of the comparison of Type II collagen mRNA expression in cells seeded onto the scaffolds with control culture in plastic showed a down regulation of this gene at 14 days that was more marked in ASCs. On the contrary, after 21 days the production of Type II collagen increased for both tested cytotypes (Figure 6A).

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.