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Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Histograms depict changes between PDPCs and PDL-SCs mRNA expression of runx2, osteonectin (sparc) and osteocalcin (bglap) observed after culturing cells for 14 and 21 days on GEL/HA with osteogenic differentiating medium. (A) Fold-changes of PDPCs and PDL-SCs seeded on scaffold with respect to PDPCs and PDL-SCs control cultures (i.e., PDPCs and PDL-SCs in tissue culture plates with osteogenic differentiating medium); (B) Fold changes of PDPCs and PDL-SCs seeded on GEL/HA (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
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Figure 5: Histograms depict changes between PDPCs and PDL-SCs mRNA expression of runx2, osteonectin (sparc) and osteocalcin (bglap) observed after culturing cells for 14 and 21 days on GEL/HA with osteogenic differentiating medium. (A) Fold-changes of PDPCs and PDL-SCs seeded on scaffold with respect to PDPCs and PDL-SCs control cultures (i.e., PDPCs and PDL-SCs in tissue culture plates with osteogenic differentiating medium); (B) Fold changes of PDPCs and PDL-SCs seeded on GEL/HA (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).

Mentions: Comparison of gene expression results in cells seeded onto GEL/HA with control culture in plastic are shown in Figure 5A. Both PDPCs and PDL-SCs showed a reduction in the expression of runx2 after 14 days of culture on GEL/HA, that was detected also after 21 days of culture, being significantly marked in PDPCs. The same trend was observed in PDPCs for osteonectin (sparc) mRNA expression. In PDL-SCs we observed a reduction of mRNA for osteonectin (Fold regulation = −1.5±0.1) after 14 days of culture and its increase (Fold regulation = 2.8 ± 0.6) after 21 days. As far as osteocalcin (bglap) mRNA expression is concerned, PDPCs showed its moderate up regulation after 14 days of culture, that became significantly marked after 21 days (Fold regulation = 6.6 ± 1.2). On the contrary, in PDL-SCs bglap mRNAexpression was down regulated at both time point analyzed with a significant decrease after 21 days of culture.


Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Histograms depict changes between PDPCs and PDL-SCs mRNA expression of runx2, osteonectin (sparc) and osteocalcin (bglap) observed after culturing cells for 14 and 21 days on GEL/HA with osteogenic differentiating medium. (A) Fold-changes of PDPCs and PDL-SCs seeded on scaffold with respect to PDPCs and PDL-SCs control cultures (i.e., PDPCs and PDL-SCs in tissue culture plates with osteogenic differentiating medium); (B) Fold changes of PDPCs and PDL-SCs seeded on GEL/HA (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585109&req=5

Figure 5: Histograms depict changes between PDPCs and PDL-SCs mRNA expression of runx2, osteonectin (sparc) and osteocalcin (bglap) observed after culturing cells for 14 and 21 days on GEL/HA with osteogenic differentiating medium. (A) Fold-changes of PDPCs and PDL-SCs seeded on scaffold with respect to PDPCs and PDL-SCs control cultures (i.e., PDPCs and PDL-SCs in tissue culture plates with osteogenic differentiating medium); (B) Fold changes of PDPCs and PDL-SCs seeded on GEL/HA (scaffold) or in tissue control plates (control) at 14 vs. 21 days. Data are expressed as fold-regulation which represents fold-change results in a biologically expressive manner (see Materials and Method section). Statistical differences with relative controls are denoted with an asterisk (*p < 0.05).
Mentions: Comparison of gene expression results in cells seeded onto GEL/HA with control culture in plastic are shown in Figure 5A. Both PDPCs and PDL-SCs showed a reduction in the expression of runx2 after 14 days of culture on GEL/HA, that was detected also after 21 days of culture, being significantly marked in PDPCs. The same trend was observed in PDPCs for osteonectin (sparc) mRNA expression. In PDL-SCs we observed a reduction of mRNA for osteonectin (Fold regulation = −1.5±0.1) after 14 days of culture and its increase (Fold regulation = 2.8 ± 0.6) after 21 days. As far as osteocalcin (bglap) mRNA expression is concerned, PDPCs showed its moderate up regulation after 14 days of culture, that became significantly marked after 21 days (Fold regulation = 6.6 ± 1.2). On the contrary, in PDL-SCs bglap mRNAexpression was down regulated at both time point analyzed with a significant decrease after 21 days of culture.

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.