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Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Related in: MedlinePlus

TEM micrographs of (A) PDL-SCs cultured on gelatin/hydroxyapatite (white asterisks) for 14 days (bar: 5 μm); (B) Fibrils (arrows) resembling the early deposition of extracellular matrix components were detected (bar: 1000 nm). The inset shows a detail on collagen type I fibers (bar: 200 nm); (C) PDL-MSCs cultured on gelatin/hydroxyapatite for 21 days (bar: 2000 nm); (D) A high synthesis of extracellular matrix components (arrows) was observed (bar: 200 nm). The inset shows details on collagen type I fibers detected in the extracellular matrix (bar: 200 nm).
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Figure 3: TEM micrographs of (A) PDL-SCs cultured on gelatin/hydroxyapatite (white asterisks) for 14 days (bar: 5 μm); (B) Fibrils (arrows) resembling the early deposition of extracellular matrix components were detected (bar: 1000 nm). The inset shows a detail on collagen type I fibers (bar: 200 nm); (C) PDL-MSCs cultured on gelatin/hydroxyapatite for 21 days (bar: 2000 nm); (D) A high synthesis of extracellular matrix components (arrows) was observed (bar: 200 nm). The inset shows details on collagen type I fibers detected in the extracellular matrix (bar: 200 nm).

Mentions: ASCs cultured for 14 days on gelatin scaffold showed a good cell adhesion on the surface of the material (Figure 3A). Nucleus and nucleolus were well-evident (Figure 3A) and a good production of extracellular matrix (ECM) components was observed (Figure 3B).


Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

TEM micrographs of (A) PDL-SCs cultured on gelatin/hydroxyapatite (white asterisks) for 14 days (bar: 5 μm); (B) Fibrils (arrows) resembling the early deposition of extracellular matrix components were detected (bar: 1000 nm). The inset shows a detail on collagen type I fibers (bar: 200 nm); (C) PDL-MSCs cultured on gelatin/hydroxyapatite for 21 days (bar: 2000 nm); (D) A high synthesis of extracellular matrix components (arrows) was observed (bar: 200 nm). The inset shows details on collagen type I fibers detected in the extracellular matrix (bar: 200 nm).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585109&req=5

Figure 3: TEM micrographs of (A) PDL-SCs cultured on gelatin/hydroxyapatite (white asterisks) for 14 days (bar: 5 μm); (B) Fibrils (arrows) resembling the early deposition of extracellular matrix components were detected (bar: 1000 nm). The inset shows a detail on collagen type I fibers (bar: 200 nm); (C) PDL-MSCs cultured on gelatin/hydroxyapatite for 21 days (bar: 2000 nm); (D) A high synthesis of extracellular matrix components (arrows) was observed (bar: 200 nm). The inset shows details on collagen type I fibers detected in the extracellular matrix (bar: 200 nm).
Mentions: ASCs cultured for 14 days on gelatin scaffold showed a good cell adhesion on the surface of the material (Figure 3A). Nucleus and nucleolus were well-evident (Figure 3A) and a good production of extracellular matrix (ECM) components was observed (Figure 3B).

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Related in: MedlinePlus