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Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


SEM micrographs of cells cultured on GEL/HA (A,B) and GEL (C,D) displaying a good colonization of the 3D scaffolds. At 21 days (insets), spherical structures (arrows) were present in PDPCs cultured on both tested scaffolds. Similar features were evidenced also in PDL-SCs seeded on GEL/HA (inset Figure 1C).
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Figure 2: SEM micrographs of cells cultured on GEL/HA (A,B) and GEL (C,D) displaying a good colonization of the 3D scaffolds. At 21 days (insets), spherical structures (arrows) were present in PDPCs cultured on both tested scaffolds. Similar features were evidenced also in PDL-SCs seeded on GEL/HA (inset Figure 1C).

Mentions: Morphological analyses showed the ability of cells to colonize the porosity of both tested scaffolds (Figure 2). After 21 days (Figure 2 insets) cells covered the entire surface of the scaffolds, and on PDL-SCs features indicative of induction of mineralization were detected.


Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

SEM micrographs of cells cultured on GEL/HA (A,B) and GEL (C,D) displaying a good colonization of the 3D scaffolds. At 21 days (insets), spherical structures (arrows) were present in PDPCs cultured on both tested scaffolds. Similar features were evidenced also in PDL-SCs seeded on GEL/HA (inset Figure 1C).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585109&req=5

Figure 2: SEM micrographs of cells cultured on GEL/HA (A,B) and GEL (C,D) displaying a good colonization of the 3D scaffolds. At 21 days (insets), spherical structures (arrows) were present in PDPCs cultured on both tested scaffolds. Similar features were evidenced also in PDL-SCs seeded on GEL/HA (inset Figure 1C).
Mentions: Morphological analyses showed the ability of cells to colonize the porosity of both tested scaffolds (Figure 2). After 21 days (Figure 2 insets) cells covered the entire surface of the scaffolds, and on PDL-SCs features indicative of induction of mineralization were detected.

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.