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Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Related in: MedlinePlus

Histograms depict MTT viability test performed in PDPCs, PDL-SCs, and ASCs cultured in tissue culture plates for 7, 14, and 21 days in osteogenic (A) or chondrogenic (B) medium. Data are expressed respectively as percentage over PDPCs, PDL-SCs, and ASCs cultured for 7, 14, and 21 days in undifferentiating medium; (C) PDPCs and PDL-SCs cultured on GEL/HA scaffolds for 7, 14, and 21 days in osteogenic medium. Data are expressed respectively as percentage over PDPCs and PDL-SCs cultured in osteogenic media in tissue culture plates in osteogenic media. (D) PDPCs and ASCs cultured on GEL scaffolds for 7, 14, and 21 days in chondrogenic medium. Mean values ± SD are reported.
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Figure 1: Histograms depict MTT viability test performed in PDPCs, PDL-SCs, and ASCs cultured in tissue culture plates for 7, 14, and 21 days in osteogenic (A) or chondrogenic (B) medium. Data are expressed respectively as percentage over PDPCs, PDL-SCs, and ASCs cultured for 7, 14, and 21 days in undifferentiating medium; (C) PDPCs and PDL-SCs cultured on GEL/HA scaffolds for 7, 14, and 21 days in osteogenic medium. Data are expressed respectively as percentage over PDPCs and PDL-SCs cultured in osteogenic media in tissue culture plates in osteogenic media. (D) PDPCs and ASCs cultured on GEL scaffolds for 7, 14, and 21 days in chondrogenic medium. Mean values ± SD are reported.

Mentions: Cell viability was assessed in PDPCs, ASCs, and PDL-SCs after 7, 14, and 21 days of culture in basal and differentiating osteogenic or chondrogenic media. Cell viability was also evaluated in cells cultured on GEL and GEL/HA scaffolds. Briefly, after removing the culture media, 200 μL of MTT (3-dimethylthiazol-2,5-diiphenyltetrazolium bromide, 135038 Sigma-Aldrich) solution (5 mg/mL in phenol red-free DMEM) and 1.8 mL DMEM were added to the multi-well plates and incubated at 37°C for 3 h. After discarding the supernatants, 2 mL of solvent (4%HCl 1N in isopropanol absolute) were added to dissolve the dark blue formazan crystals and quantify them spectrophotometrically measuring the absorbance at 570 nm (Secoman, Anthelie light, version 3.8, Contardi, Italy). Data were expressed as percentage over the respective control culture (see Figure 1 legend).


Stem cell origin differently affects bone tissue engineering strategies.

Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M - Front Physiol (2015)

Histograms depict MTT viability test performed in PDPCs, PDL-SCs, and ASCs cultured in tissue culture plates for 7, 14, and 21 days in osteogenic (A) or chondrogenic (B) medium. Data are expressed respectively as percentage over PDPCs, PDL-SCs, and ASCs cultured for 7, 14, and 21 days in undifferentiating medium; (C) PDPCs and PDL-SCs cultured on GEL/HA scaffolds for 7, 14, and 21 days in osteogenic medium. Data are expressed respectively as percentage over PDPCs and PDL-SCs cultured in osteogenic media in tissue culture plates in osteogenic media. (D) PDPCs and ASCs cultured on GEL scaffolds for 7, 14, and 21 days in chondrogenic medium. Mean values ± SD are reported.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4585109&req=5

Figure 1: Histograms depict MTT viability test performed in PDPCs, PDL-SCs, and ASCs cultured in tissue culture plates for 7, 14, and 21 days in osteogenic (A) or chondrogenic (B) medium. Data are expressed respectively as percentage over PDPCs, PDL-SCs, and ASCs cultured for 7, 14, and 21 days in undifferentiating medium; (C) PDPCs and PDL-SCs cultured on GEL/HA scaffolds for 7, 14, and 21 days in osteogenic medium. Data are expressed respectively as percentage over PDPCs and PDL-SCs cultured in osteogenic media in tissue culture plates in osteogenic media. (D) PDPCs and ASCs cultured on GEL scaffolds for 7, 14, and 21 days in chondrogenic medium. Mean values ± SD are reported.
Mentions: Cell viability was assessed in PDPCs, ASCs, and PDL-SCs after 7, 14, and 21 days of culture in basal and differentiating osteogenic or chondrogenic media. Cell viability was also evaluated in cells cultured on GEL and GEL/HA scaffolds. Briefly, after removing the culture media, 200 μL of MTT (3-dimethylthiazol-2,5-diiphenyltetrazolium bromide, 135038 Sigma-Aldrich) solution (5 mg/mL in phenol red-free DMEM) and 1.8 mL DMEM were added to the multi-well plates and incubated at 37°C for 3 h. After discarding the supernatants, 2 mL of solvent (4%HCl 1N in isopropanol absolute) were added to dissolve the dark blue formazan crystals and quantify them spectrophotometrically measuring the absorbance at 570 nm (Secoman, Anthelie light, version 3.8, Contardi, Italy). Data were expressed as percentage over the respective control culture (see Figure 1 legend).

Bottom Line: Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes.The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue.We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche Ancona, Italy.

ABSTRACT
Bone tissue engineering approaches are encouraging for the improvement of conventional bone grafting technique drawbacks. Thanks to their self-renewal and multi-lineage differentiation ability, stem cells are one of the major actors in tissue engineering approaches, and among these adult mesenchymal stem cells (MSCs) hold a great promise for regenerative medicine strategies. Bone marrow MSCs (BM-MSCs) are the first- identified and well-recognized stem cell population used in bone tissue engineering. Nevertheless, several factors hamper BM-MSC clinical application and subsequently, new stem cell sources have been investigated for these purposes. The fruitful selection and combination of tissue engineered scaffold, progenitor cells, and physiologic signaling molecules allowed the surgeon to reconstruct the missing natural tissue. On the basis of these considerations, we analyzed the capability of two different scaffolds, planned for osteochondral tissue regeneration, to modulate differentiation of adult stem cells of dissimilar local sources (i.e., periodontal ligament, maxillary periosteum) as well as adipose-derived stem cells (ASCs), in view of possible craniofacial tissue engineering strategies. We demonstrated that cells are differently committed toward the osteoblastic phenotype and therefore, taking into account their specific features, they could be intriguing cell sources in different stem cell-based bone/periodontal tissue regeneration approaches.

No MeSH data available.


Related in: MedlinePlus