Limits...
Common protein sequence signatures associate with Sclerotinia borealis lifestyle and secretion in fungal pathogens of the Sclerotiniaceae.

Badet T, Peyraud R, Raffaele S - Front Plant Sci (2015)

Bottom Line: To spread successfully, S. borealis must synthesize proteins adapted to function in its specific environment.We found that enrichment in Thr, depletion in Glu and Lys, and low disorder frequency in hot loops are significantly associated with S. borealis proteins.High index proteins were also enriched in function associated with plant colonization in S. borealis, and in in planta-induced genes in S. sclerotiorum.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Interactions Plantes-Microorganismes, Institut National de la Recherche Agronomique, UMR441 Castanet-Tolosan, France ; Laboratoire des Interactions Plantes-Microorganismes, Centre National de la Recherche Scientifique, UMR2594 Castanet-Tolosan, France.

ABSTRACT
Fungal plant pathogens produce secreted proteins adapted to function outside fungal cells to facilitate colonization of their hosts. In many cases such as for fungi from the Sclerotiniaceae family the repertoire and function of secreted proteins remains elusive. In the Sclerotiniaceae, whereas Sclerotinia sclerotiorum and Botrytis cinerea are cosmopolitan broad host-range plant pathogens, Sclerotinia borealis has a psychrophilic lifestyle with a low optimal growth temperature, a narrow host range and geographic distribution. To spread successfully, S. borealis must synthesize proteins adapted to function in its specific environment. The search for signatures of adaptation to S. borealis lifestyle may therefore help revealing proteins critical for colonization of the environment by Sclerotiniaceae fungi. Here, we analyzed amino acids usage and intrinsic protein disorder in alignments of groups of orthologous proteins from the three Sclerotiniaceae species. We found that enrichment in Thr, depletion in Glu and Lys, and low disorder frequency in hot loops are significantly associated with S. borealis proteins. We designed an index to report bias in these properties and found that high index proteins were enriched among secreted proteins in the three Sclerotiniaceae fungi. High index proteins were also enriched in function associated with plant colonization in S. borealis, and in in planta-induced genes in S. sclerotiorum. We highlight a novel putative antifreeze protein and a novel putative lytic polysaccharide monooxygenase identified through our pipeline as candidate proteins involved in colonization of the environment. Our findings suggest that similar protein signatures associate with S. borealis lifestyle and with secretion in the Sclerotiniaceae. These signatures may be useful for identifying proteins of interest as targets for the management of plant diseases.

No MeSH data available.


Related in: MedlinePlus

Sclerotinia borealis colonizes different niches than its close relatives S. sclerotiorum and Botrytis cinerea. Number of host plant genera (A) and geographic distribution (B) of the three fungal species according to the USDA Systematic Mycology and Microbiology Laboratory Fungus-Host Database (Farr and Rossman, 2015).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585107&req=5

Figure 1: Sclerotinia borealis colonizes different niches than its close relatives S. sclerotiorum and Botrytis cinerea. Number of host plant genera (A) and geographic distribution (B) of the three fungal species according to the USDA Systematic Mycology and Microbiology Laboratory Fungus-Host Database (Farr and Rossman, 2015).

Mentions: Fungi from the Sclerotiniaceae family include several devastating plant pathogens with a broad host range. Among those are Botrytis cinerea, the causal agent of gray rot, and Sclerotinia sclerotiorum, causal agent of white and stem rot, each able to infect several hundreds of plant genera and causing multi-billion dollar losses in agriculture every year (Figure 1A) (Bolton et al., 2006; Dean et al., 2012). The geographic distribution of these two fungi is also remarkably broad since they have been reported across five continents (Figure 1B). Sequencing of the genome of B. cinerea and S. sclerotiorum (Amselem et al., 2011) opened the way to systematic searches for the molecular bases of pathogenicity in these fungi (Guyon et al., 2014; Heard et al., 2015). However, the repertoire of molecules contributing to the ability of plant pathogenic fungi, such as fungi from the Sclerotiniaceae family, to colonize a wide range of hosts and environments remains elusive.


Common protein sequence signatures associate with Sclerotinia borealis lifestyle and secretion in fungal pathogens of the Sclerotiniaceae.

Badet T, Peyraud R, Raffaele S - Front Plant Sci (2015)

Sclerotinia borealis colonizes different niches than its close relatives S. sclerotiorum and Botrytis cinerea. Number of host plant genera (A) and geographic distribution (B) of the three fungal species according to the USDA Systematic Mycology and Microbiology Laboratory Fungus-Host Database (Farr and Rossman, 2015).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585107&req=5

Figure 1: Sclerotinia borealis colonizes different niches than its close relatives S. sclerotiorum and Botrytis cinerea. Number of host plant genera (A) and geographic distribution (B) of the three fungal species according to the USDA Systematic Mycology and Microbiology Laboratory Fungus-Host Database (Farr and Rossman, 2015).
Mentions: Fungi from the Sclerotiniaceae family include several devastating plant pathogens with a broad host range. Among those are Botrytis cinerea, the causal agent of gray rot, and Sclerotinia sclerotiorum, causal agent of white and stem rot, each able to infect several hundreds of plant genera and causing multi-billion dollar losses in agriculture every year (Figure 1A) (Bolton et al., 2006; Dean et al., 2012). The geographic distribution of these two fungi is also remarkably broad since they have been reported across five continents (Figure 1B). Sequencing of the genome of B. cinerea and S. sclerotiorum (Amselem et al., 2011) opened the way to systematic searches for the molecular bases of pathogenicity in these fungi (Guyon et al., 2014; Heard et al., 2015). However, the repertoire of molecules contributing to the ability of plant pathogenic fungi, such as fungi from the Sclerotiniaceae family, to colonize a wide range of hosts and environments remains elusive.

Bottom Line: To spread successfully, S. borealis must synthesize proteins adapted to function in its specific environment.We found that enrichment in Thr, depletion in Glu and Lys, and low disorder frequency in hot loops are significantly associated with S. borealis proteins.High index proteins were also enriched in function associated with plant colonization in S. borealis, and in in planta-induced genes in S. sclerotiorum.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Interactions Plantes-Microorganismes, Institut National de la Recherche Agronomique, UMR441 Castanet-Tolosan, France ; Laboratoire des Interactions Plantes-Microorganismes, Centre National de la Recherche Scientifique, UMR2594 Castanet-Tolosan, France.

ABSTRACT
Fungal plant pathogens produce secreted proteins adapted to function outside fungal cells to facilitate colonization of their hosts. In many cases such as for fungi from the Sclerotiniaceae family the repertoire and function of secreted proteins remains elusive. In the Sclerotiniaceae, whereas Sclerotinia sclerotiorum and Botrytis cinerea are cosmopolitan broad host-range plant pathogens, Sclerotinia borealis has a psychrophilic lifestyle with a low optimal growth temperature, a narrow host range and geographic distribution. To spread successfully, S. borealis must synthesize proteins adapted to function in its specific environment. The search for signatures of adaptation to S. borealis lifestyle may therefore help revealing proteins critical for colonization of the environment by Sclerotiniaceae fungi. Here, we analyzed amino acids usage and intrinsic protein disorder in alignments of groups of orthologous proteins from the three Sclerotiniaceae species. We found that enrichment in Thr, depletion in Glu and Lys, and low disorder frequency in hot loops are significantly associated with S. borealis proteins. We designed an index to report bias in these properties and found that high index proteins were enriched among secreted proteins in the three Sclerotiniaceae fungi. High index proteins were also enriched in function associated with plant colonization in S. borealis, and in in planta-induced genes in S. sclerotiorum. We highlight a novel putative antifreeze protein and a novel putative lytic polysaccharide monooxygenase identified through our pipeline as candidate proteins involved in colonization of the environment. Our findings suggest that similar protein signatures associate with S. borealis lifestyle and with secretion in the Sclerotiniaceae. These signatures may be useful for identifying proteins of interest as targets for the management of plant diseases.

No MeSH data available.


Related in: MedlinePlus