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Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus

Thylakoid protein phosphorylation after wheat stripe rust infection of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Thylakoid proteins extracted from the inoculated and un-inoculated wheat plants were fractionated by SDS-PAGE in 12% acrylamide separation gel with 6 M urea. Immunoblot analysis of thylakoid membrane proteins was performed using anti-phosphothreonine antibodies (A). Loading was based on an equal amount of chlorophyll (1 μg chlorophyll). The SDS-PAGE results after Coomassie blue staining (CBS) are shown in the bottom panel (B). CK, un-inoculated wheat plants. (C) Quantification of immunoblot data. Results are presented relative to the amount of respective CK (100%). Asterisks indicates statistically significant differences at the P < 0.05 level. Values are means ± SD from three independent biological replicates.
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Figure 8: Thylakoid protein phosphorylation after wheat stripe rust infection of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Thylakoid proteins extracted from the inoculated and un-inoculated wheat plants were fractionated by SDS-PAGE in 12% acrylamide separation gel with 6 M urea. Immunoblot analysis of thylakoid membrane proteins was performed using anti-phosphothreonine antibodies (A). Loading was based on an equal amount of chlorophyll (1 μg chlorophyll). The SDS-PAGE results after Coomassie blue staining (CBS) are shown in the bottom panel (B). CK, un-inoculated wheat plants. (C) Quantification of immunoblot data. Results are presented relative to the amount of respective CK (100%). Asterisks indicates statistically significant differences at the P < 0.05 level. Values are means ± SD from three independent biological replicates.

Mentions: To determine whether a reduction in the abundance of PSII subunits occurred in the susceptible wheat, the thylakoid polypeptide composition was investigated by the immuno-blotting (Figure 7). The levels of four major PSI proteins showed no detectable changes after the infection (Figure 7A). Although there was no obvious difference in the level of almost all PSII proteins between inoculated and un-inoculated wheat, changes in the D1 and Lhcb4 (CP29) proteins were observed in Sy95-71 and CN19 after the infection (Figure 7B). Compared with the control, infection resulted in a decrease in the D1 protein in Sy95-71. By contrast, the level of D1 was increased in CN19 after the infection compared with the control. We also found that the level of the D2 protein showed no obvious difference between Sy95-71 and CN19 (Figure 7A). Interestingly, although the level of the CP29 protein did not change in any of the plants, CP29 protein was phosphorylated in inoculated wheat plants, especially in CN19 (Figure 7B). Further analyses of thylakoid membrane protein phosphorylation were performed with the anti-phosphothreonine antibody. As shown in Figure 8A, thylakoid protein phosphorylation did not show obvious differences after the infection, although phosphorylated-D1 (P-D1) and P-LHCII presented some differences. In Sy95-71, the infection resulted in a decrease in the level of P-D1 and P-LHCII compared with the control plants. In contrast, more levels of phosphorylated D1 and light-harvesting complex II (LHCII) were found in the infected CN19 than that of the non-inoculated control (Figure 8).


Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Thylakoid protein phosphorylation after wheat stripe rust infection of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Thylakoid proteins extracted from the inoculated and un-inoculated wheat plants were fractionated by SDS-PAGE in 12% acrylamide separation gel with 6 M urea. Immunoblot analysis of thylakoid membrane proteins was performed using anti-phosphothreonine antibodies (A). Loading was based on an equal amount of chlorophyll (1 μg chlorophyll). The SDS-PAGE results after Coomassie blue staining (CBS) are shown in the bottom panel (B). CK, un-inoculated wheat plants. (C) Quantification of immunoblot data. Results are presented relative to the amount of respective CK (100%). Asterisks indicates statistically significant differences at the P < 0.05 level. Values are means ± SD from three independent biological replicates.
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Related In: Results  -  Collection

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Figure 8: Thylakoid protein phosphorylation after wheat stripe rust infection of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Thylakoid proteins extracted from the inoculated and un-inoculated wheat plants were fractionated by SDS-PAGE in 12% acrylamide separation gel with 6 M urea. Immunoblot analysis of thylakoid membrane proteins was performed using anti-phosphothreonine antibodies (A). Loading was based on an equal amount of chlorophyll (1 μg chlorophyll). The SDS-PAGE results after Coomassie blue staining (CBS) are shown in the bottom panel (B). CK, un-inoculated wheat plants. (C) Quantification of immunoblot data. Results are presented relative to the amount of respective CK (100%). Asterisks indicates statistically significant differences at the P < 0.05 level. Values are means ± SD from three independent biological replicates.
Mentions: To determine whether a reduction in the abundance of PSII subunits occurred in the susceptible wheat, the thylakoid polypeptide composition was investigated by the immuno-blotting (Figure 7). The levels of four major PSI proteins showed no detectable changes after the infection (Figure 7A). Although there was no obvious difference in the level of almost all PSII proteins between inoculated and un-inoculated wheat, changes in the D1 and Lhcb4 (CP29) proteins were observed in Sy95-71 and CN19 after the infection (Figure 7B). Compared with the control, infection resulted in a decrease in the D1 protein in Sy95-71. By contrast, the level of D1 was increased in CN19 after the infection compared with the control. We also found that the level of the D2 protein showed no obvious difference between Sy95-71 and CN19 (Figure 7A). Interestingly, although the level of the CP29 protein did not change in any of the plants, CP29 protein was phosphorylated in inoculated wheat plants, especially in CN19 (Figure 7B). Further analyses of thylakoid membrane protein phosphorylation were performed with the anti-phosphothreonine antibody. As shown in Figure 8A, thylakoid protein phosphorylation did not show obvious differences after the infection, although phosphorylated-D1 (P-D1) and P-LHCII presented some differences. In Sy95-71, the infection resulted in a decrease in the level of P-D1 and P-LHCII compared with the control plants. In contrast, more levels of phosphorylated D1 and light-harvesting complex II (LHCII) were found in the infected CN19 than that of the non-inoculated control (Figure 8).

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus