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Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus

Effects of stripe rust infection on the POD, Peroxidase (A); SOD, superoxide dismutase (B); catalase CAT, catalase; (C); APX, ascorbate peroxidase (D); GPX, glutathione peroxidase (E); and GR, glutathione reductase (F) in the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
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Figure 5: Effects of stripe rust infection on the POD, Peroxidase (A); SOD, superoxide dismutase (B); catalase CAT, catalase; (C); APX, ascorbate peroxidase (D); GPX, glutathione peroxidase (E); and GR, glutathione reductase (F) in the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.

Mentions: The effects of Pst infection on the activities of antioxidant enzymes in the susceptible and resistant wheat are presented in Figure 5. The antioxidant enzyme activities in the un-inoculated plants of Sy95-71 and CN19 showed no significant difference (Figure 5). After inoculation, the POD, CAT, and GPX activities increased in Sy95-71 and CN19 compared with their respective controls. However, a more obvious increase was observed in CN19, especially with respect to GPX activity. In Sy95-71 and CN19, infections caused significant increases in GPX activities of 41 and 79%, respectively (Figure 5E). However, after infection, SOD activity decreased slightly in Sy95-71 but was not significantly changed in CN19 compared with the respective controls (Figure 5B). In addition, we found that APX and GR activities decreased in Sy95-71 and CN19 infected with Pst (Figures 5D,F). A more pronounced decrease was observed in Sy95-71 after infection. There was a 50% decrease in GR activity in Sy95-71 exposed to the stripe rust (Figure 5F).


Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Effects of stripe rust infection on the POD, Peroxidase (A); SOD, superoxide dismutase (B); catalase CAT, catalase; (C); APX, ascorbate peroxidase (D); GPX, glutathione peroxidase (E); and GR, glutathione reductase (F) in the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585106&req=5

Figure 5: Effects of stripe rust infection on the POD, Peroxidase (A); SOD, superoxide dismutase (B); catalase CAT, catalase; (C); APX, ascorbate peroxidase (D); GPX, glutathione peroxidase (E); and GR, glutathione reductase (F) in the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
Mentions: The effects of Pst infection on the activities of antioxidant enzymes in the susceptible and resistant wheat are presented in Figure 5. The antioxidant enzyme activities in the un-inoculated plants of Sy95-71 and CN19 showed no significant difference (Figure 5). After inoculation, the POD, CAT, and GPX activities increased in Sy95-71 and CN19 compared with their respective controls. However, a more obvious increase was observed in CN19, especially with respect to GPX activity. In Sy95-71 and CN19, infections caused significant increases in GPX activities of 41 and 79%, respectively (Figure 5E). However, after infection, SOD activity decreased slightly in Sy95-71 but was not significantly changed in CN19 compared with the respective controls (Figure 5B). In addition, we found that APX and GR activities decreased in Sy95-71 and CN19 infected with Pst (Figures 5D,F). A more pronounced decrease was observed in Sy95-71 after infection. There was a 50% decrease in GR activity in Sy95-71 exposed to the stripe rust (Figure 5F).

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus