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Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus

Effects of stripe rust infection on the malondialdehyde (MDA) content (A) and electrolyte leakage (B) of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
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Figure 4: Effects of stripe rust infection on the malondialdehyde (MDA) content (A) and electrolyte leakage (B) of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.

Mentions: Quantitative levels of H2O2 and produced in the inoculated and un-inoculated leaves were determined further. After infection, the level of accumulation in CN19 was slightly higher than that observed in the control plants (Figure 3C). In Sy95-71, stripe rust fungal inoculation resulted in a significant increase in the content compared with that of the controls and CN19 (P < 0.05). Similar results were observed for the level of H2O2. and H2O2 levels in Sy95-71 increased by 43 and 284%, respectively, after inoculation compared with the control (Figures 3C,D). In addition, we examined the degree of oxidative damage in leaves of wheat subjected to Pst infection by determining the amount of lipid peroxidation (Barclay and Mckersie, 1994). As shown in Figure 4A, the stripe rust fungal infection raised the concentration of MDA about 29 and 47% in CN19 and Sy95-71, respectively, when compared with the respective controls. Similarly, the electrolyte leakage in inoculated Sy95-71 was also higher than that of inoculated CN19 (Figure 4B).


Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Effects of stripe rust infection on the malondialdehyde (MDA) content (A) and electrolyte leakage (B) of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585106&req=5

Figure 4: Effects of stripe rust infection on the malondialdehyde (MDA) content (A) and electrolyte leakage (B) of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Bars represent standard deviations of three independent biological replicates and values followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. CK, un-inoculated wheat plants.
Mentions: Quantitative levels of H2O2 and produced in the inoculated and un-inoculated leaves were determined further. After infection, the level of accumulation in CN19 was slightly higher than that observed in the control plants (Figure 3C). In Sy95-71, stripe rust fungal inoculation resulted in a significant increase in the content compared with that of the controls and CN19 (P < 0.05). Similar results were observed for the level of H2O2. and H2O2 levels in Sy95-71 increased by 43 and 284%, respectively, after inoculation compared with the control (Figures 3C,D). In addition, we examined the degree of oxidative damage in leaves of wheat subjected to Pst infection by determining the amount of lipid peroxidation (Barclay and Mckersie, 1994). As shown in Figure 4A, the stripe rust fungal infection raised the concentration of MDA about 29 and 47% in CN19 and Sy95-71, respectively, when compared with the respective controls. Similarly, the electrolyte leakage in inoculated Sy95-71 was also higher than that of inoculated CN19 (Figure 4B).

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus