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Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus

Measurement of reactive oxygen species (ROS) after stripe rust infection. Histochemical assays for superoxide anion radicals () and hydrogen peroxide (H2O2) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. Then,  production (C) and the H2O2 content (D) were measured. Values are means ± SD from three independent biological replicates. Different letters depict significant differences between the susceptible (Sy95-71) and resistant (CN19) wheat cultivars (P < 0.05). CK, un-inoculated wheat plants.
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Figure 3: Measurement of reactive oxygen species (ROS) after stripe rust infection. Histochemical assays for superoxide anion radicals () and hydrogen peroxide (H2O2) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. Then, production (C) and the H2O2 content (D) were measured. Values are means ± SD from three independent biological replicates. Different letters depict significant differences between the susceptible (Sy95-71) and resistant (CN19) wheat cultivars (P < 0.05). CK, un-inoculated wheat plants.

Mentions: The induction of and H2O2 by Pst inoculation in the CN19 and Sy95-71 plants at the boot stage was analyzed by histochemical staining with NBT and DAB, respectively. No significant difference was detected among the controls of CN19 and Sy95-71 (Figures 3A,B). Interestingly, both and H2O2 were upregulated in infected leaves in both susceptible and resistant wheat, compared with the control plants. In comparison, the accumulation of and H2O2 was more pronounced in the infected Sy95-71 than that of the infected CN19.


Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Measurement of reactive oxygen species (ROS) after stripe rust infection. Histochemical assays for superoxide anion radicals () and hydrogen peroxide (H2O2) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. Then,  production (C) and the H2O2 content (D) were measured. Values are means ± SD from three independent biological replicates. Different letters depict significant differences between the susceptible (Sy95-71) and resistant (CN19) wheat cultivars (P < 0.05). CK, un-inoculated wheat plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585106&req=5

Figure 3: Measurement of reactive oxygen species (ROS) after stripe rust infection. Histochemical assays for superoxide anion radicals () and hydrogen peroxide (H2O2) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. Then, production (C) and the H2O2 content (D) were measured. Values are means ± SD from three independent biological replicates. Different letters depict significant differences between the susceptible (Sy95-71) and resistant (CN19) wheat cultivars (P < 0.05). CK, un-inoculated wheat plants.
Mentions: The induction of and H2O2 by Pst inoculation in the CN19 and Sy95-71 plants at the boot stage was analyzed by histochemical staining with NBT and DAB, respectively. No significant difference was detected among the controls of CN19 and Sy95-71 (Figures 3A,B). Interestingly, both and H2O2 were upregulated in infected leaves in both susceptible and resistant wheat, compared with the control plants. In comparison, the accumulation of and H2O2 was more pronounced in the infected Sy95-71 than that of the infected CN19.

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus