Limits...
Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll (A), net photosynthetic rate (B), relative water content (RWC) (C), and total protein content (D) in inoculated and un-inoculated leaves of Sy95-71 and CN19. Error bars represent the standard deviation based on three biological replicates. Different letters depict significant differences between the susceptible and resistant wheat cultivars (P < 0.05). Statistical analysis was performed using one-way ANOVA followed by Duncan’s multiple range test. CK, un-inoculated wheat plants.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585106&req=5

Figure 2: Chlorophyll (A), net photosynthetic rate (B), relative water content (RWC) (C), and total protein content (D) in inoculated and un-inoculated leaves of Sy95-71 and CN19. Error bars represent the standard deviation based on three biological replicates. Different letters depict significant differences between the susceptible and resistant wheat cultivars (P < 0.05). Statistical analysis was performed using one-way ANOVA followed by Duncan’s multiple range test. CK, un-inoculated wheat plants.

Mentions: There was no significant difference in the Chl, Pn, RWC, and total protein content between the control plants of the susceptible and resistant wheat (Figure 2). In CN19, the wheat stripe rust fungal infection caused a decrease in the Chl content of 17% compared with the CN19 control (Figure 2A). However, a marked decrease in Chl content was observed in the susceptible wheat Sy95-71 after inoculation at the boot stage, reaching 27% when compared with the Sy95-71 control. The infected Sy95-71 also had a more significant decrease in the photosynthesis rate compared with the infected CN19 at 72 hpi. Apparently, the Pst infection can regulate photosynthetic rates of infected leaves in both Sy95-71 and CN19 (Figure 2B). However, after inoculation, the RWC and total protein content were not significantly different between the susceptible and the resistant wheat (Figures 2C,D).


Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage.

Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY - Front Plant Sci (2015)

Chlorophyll (A), net photosynthetic rate (B), relative water content (RWC) (C), and total protein content (D) in inoculated and un-inoculated leaves of Sy95-71 and CN19. Error bars represent the standard deviation based on three biological replicates. Different letters depict significant differences between the susceptible and resistant wheat cultivars (P < 0.05). Statistical analysis was performed using one-way ANOVA followed by Duncan’s multiple range test. CK, un-inoculated wheat plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585106&req=5

Figure 2: Chlorophyll (A), net photosynthetic rate (B), relative water content (RWC) (C), and total protein content (D) in inoculated and un-inoculated leaves of Sy95-71 and CN19. Error bars represent the standard deviation based on three biological replicates. Different letters depict significant differences between the susceptible and resistant wheat cultivars (P < 0.05). Statistical analysis was performed using one-way ANOVA followed by Duncan’s multiple range test. CK, un-inoculated wheat plants.
Mentions: There was no significant difference in the Chl, Pn, RWC, and total protein content between the control plants of the susceptible and resistant wheat (Figure 2). In CN19, the wheat stripe rust fungal infection caused a decrease in the Chl content of 17% compared with the CN19 control (Figure 2A). However, a marked decrease in Chl content was observed in the susceptible wheat Sy95-71 after inoculation at the boot stage, reaching 27% when compared with the Sy95-71 control. The infected Sy95-71 also had a more significant decrease in the photosynthesis rate compared with the infected CN19 at 72 hpi. Apparently, the Pst infection can regulate photosynthetic rates of infected leaves in both Sy95-71 and CN19 (Figure 2B). However, after inoculation, the RWC and total protein content were not significantly different between the susceptible and the resistant wheat (Figures 2C,D).

Bottom Line: We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection.More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat.The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Sichuan Agricultural University Ya'an, China.

ABSTRACT
Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. To gain a better understanding of the protective mechanism against stripe rust at the adult plant stage, the differences in photosystem II and antioxidant enzymatic systems between susceptible and resistant wheat in response to stripe rust disease (P. striiformis) were investigated. We found that chlorophyll fluorescence and the activities of the antioxidant enzymes were higher in resistant wheat than in susceptible wheat after stripe rust infection. Compared with the susceptible wheat, the resistant wheat accumulated a higher level of D1 protein and a lower level of reactive oxygen species after infection. Furthermore, our results demonstrate that D1 and light-harvesting complex II (LHCII) phosphorylation are involved in the resistance to stripe rust in wheat. The CP29 protein was phosphorylated under stripe rust infection, like its phosphorylation in other monocots under environmental stresses. More extensive damages occur on the thylakoid membranes in the susceptible wheat compared with the resistant wheat. The findings provide evidence that thylakoid protein phosphorylation and antioxidant enzyme systems play important roles in plant responses and defense to biotic stress.

No MeSH data available.


Related in: MedlinePlus