Limits...
Salmonella Typhimurium exploits inflammation to its own advantage in piglets.

Chirullo B, Pesciaroli M, Drumo R, Ruggeri J, Razzuoli E, Pistoia C, Petrucci P, Martinelli N, Cucco L, Moscati L, Amadori M, Magistrali CF, Alborali GL, Pasquali P - Front Microbiol (2015)

Bottom Line: This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment.This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization.Typhimurium exploits inflammation for its benefit in piglets.

View Article: PubMed Central - PubMed

Affiliation: Unit of Prophyilaxis and Control of Bacterial Zoonoses, Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità Rome, Italy.

ABSTRACT
Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization. Moreover, LPS in vivo treatment increased cytokines blood level and body temperature at 4 h post infection, which is consistent with an acute inflammatory stimulus, capable to influence the colonization of STM14028 in different organs and tissues. The present study proves for the first time that in acute enteric salmonellosis, S. Typhimurium exploits inflammation for its benefit in piglets.

No MeSH data available.


Related in: MedlinePlus

STM14028 colonization of mono-macrophages and IPEC-J2 cells at 3 and 24 h post infection (A–D). STM14028 colonization increases with LPS pre-treatment and is reduced by RS-LPS antagonist to the values of LPS-untreated cells (*P ≤ 0.1; **P ≤ 0.01, One-Way Anova Turkey's multiple comparisons test, data from one representative experiment out of three with similar results).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585093&req=5

Figure 2: STM14028 colonization of mono-macrophages and IPEC-J2 cells at 3 and 24 h post infection (A–D). STM14028 colonization increases with LPS pre-treatment and is reduced by RS-LPS antagonist to the values of LPS-untreated cells (*P ≤ 0.1; **P ≤ 0.01, One-Way Anova Turkey's multiple comparisons test, data from one representative experiment out of three with similar results).

Mentions: We observed that STM14028 was able to efficiently colonize monocytes/macrophages and IPEC-J2 cells at both 3 and 24 h post STM14028-infection (Figures 2A–D). A previous treatment with purified LPS significantly increased STM14028 colonization in both cell types at 3 and 24 h after infection. When RS-LPS antagonist was used, this markedly inhibited LPS stimulation causing a colonization level similar to the one obtained by STM14028 infection alone (Figures 2A–D). Overall, these results suggest that LPS stimulation can create conditions in which STM14028 is more efficiently phagocytized by cultivated cells, within it can find suitable conditions to multiply.


Salmonella Typhimurium exploits inflammation to its own advantage in piglets.

Chirullo B, Pesciaroli M, Drumo R, Ruggeri J, Razzuoli E, Pistoia C, Petrucci P, Martinelli N, Cucco L, Moscati L, Amadori M, Magistrali CF, Alborali GL, Pasquali P - Front Microbiol (2015)

STM14028 colonization of mono-macrophages and IPEC-J2 cells at 3 and 24 h post infection (A–D). STM14028 colonization increases with LPS pre-treatment and is reduced by RS-LPS antagonist to the values of LPS-untreated cells (*P ≤ 0.1; **P ≤ 0.01, One-Way Anova Turkey's multiple comparisons test, data from one representative experiment out of three with similar results).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585093&req=5

Figure 2: STM14028 colonization of mono-macrophages and IPEC-J2 cells at 3 and 24 h post infection (A–D). STM14028 colonization increases with LPS pre-treatment and is reduced by RS-LPS antagonist to the values of LPS-untreated cells (*P ≤ 0.1; **P ≤ 0.01, One-Way Anova Turkey's multiple comparisons test, data from one representative experiment out of three with similar results).
Mentions: We observed that STM14028 was able to efficiently colonize monocytes/macrophages and IPEC-J2 cells at both 3 and 24 h post STM14028-infection (Figures 2A–D). A previous treatment with purified LPS significantly increased STM14028 colonization in both cell types at 3 and 24 h after infection. When RS-LPS antagonist was used, this markedly inhibited LPS stimulation causing a colonization level similar to the one obtained by STM14028 infection alone (Figures 2A–D). Overall, these results suggest that LPS stimulation can create conditions in which STM14028 is more efficiently phagocytized by cultivated cells, within it can find suitable conditions to multiply.

Bottom Line: This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment.This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization.Typhimurium exploits inflammation for its benefit in piglets.

View Article: PubMed Central - PubMed

Affiliation: Unit of Prophyilaxis and Control of Bacterial Zoonoses, Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità Rome, Italy.

ABSTRACT
Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization. Moreover, LPS in vivo treatment increased cytokines blood level and body temperature at 4 h post infection, which is consistent with an acute inflammatory stimulus, capable to influence the colonization of STM14028 in different organs and tissues. The present study proves for the first time that in acute enteric salmonellosis, S. Typhimurium exploits inflammation for its benefit in piglets.

No MeSH data available.


Related in: MedlinePlus