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Salmonella Typhimurium exploits inflammation to its own advantage in piglets.

Chirullo B, Pesciaroli M, Drumo R, Ruggeri J, Razzuoli E, Pistoia C, Petrucci P, Martinelli N, Cucco L, Moscati L, Amadori M, Magistrali CF, Alborali GL, Pasquali P - Front Microbiol (2015)

Bottom Line: This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment.This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization.Typhimurium exploits inflammation for its benefit in piglets.

View Article: PubMed Central - PubMed

Affiliation: Unit of Prophyilaxis and Control of Bacterial Zoonoses, Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità Rome, Italy.

ABSTRACT
Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization. Moreover, LPS in vivo treatment increased cytokines blood level and body temperature at 4 h post infection, which is consistent with an acute inflammatory stimulus, capable to influence the colonization of STM14028 in different organs and tissues. The present study proves for the first time that in acute enteric salmonellosis, S. Typhimurium exploits inflammation for its benefit in piglets.

No MeSH data available.


Related in: MedlinePlus

STM14028 infection induces an increase of innate immunity cell compartment and colonizes different organs of piglets orally infected. (A) The prevalence of CD14+, CD21+, and polymorphonuclear (PMN) cells was determined in ileo-cecal lymph nodes, 48 h post-infection with STM14028. The differences were statistically significant (***P ≤ 0.001, multiple comparisons t-test). (B) STM14028 count in ileo-cecal lymph nodes (ICLN), spleen, colon, and cecum of infected piglets. Data represent mean with error bars as SEM of six piglets per group.
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Figure 1: STM14028 infection induces an increase of innate immunity cell compartment and colonizes different organs of piglets orally infected. (A) The prevalence of CD14+, CD21+, and polymorphonuclear (PMN) cells was determined in ileo-cecal lymph nodes, 48 h post-infection with STM14028. The differences were statistically significant (***P ≤ 0.001, multiple comparisons t-test). (B) STM14028 count in ileo-cecal lymph nodes (ICLN), spleen, colon, and cecum of infected piglets. Data represent mean with error bars as SEM of six piglets per group.

Mentions: In order to verify the involvement of the innate immune response during Salmonella infection in piglets, phenotypic analysis in ileo-cecal lymph nodes was performed in piglets orally infected with STM14028 and euthanized 48 h later. As depicted in Figure 1A, it was possible to observe a higher increase in the percentage of CD14+ (mainly monocytes) and polymorphonuclear cells with a minor involvement of CD21+ B cells. Moreover, the STM14028-infection was confirmed by the bacterial count in different organs (Figure 1B). These findings are consistent with a rapid recruitment of neutrophils and monocytes/macrophages toward lymph nodes, crucial for the effective response to lipopolysaccharides stimulus or concomitant bacterial infection. These data suggest a prompt involvement of the innate immune response in the regional lymph nodes draining the gut, after an oral infection with STM14028.


Salmonella Typhimurium exploits inflammation to its own advantage in piglets.

Chirullo B, Pesciaroli M, Drumo R, Ruggeri J, Razzuoli E, Pistoia C, Petrucci P, Martinelli N, Cucco L, Moscati L, Amadori M, Magistrali CF, Alborali GL, Pasquali P - Front Microbiol (2015)

STM14028 infection induces an increase of innate immunity cell compartment and colonizes different organs of piglets orally infected. (A) The prevalence of CD14+, CD21+, and polymorphonuclear (PMN) cells was determined in ileo-cecal lymph nodes, 48 h post-infection with STM14028. The differences were statistically significant (***P ≤ 0.001, multiple comparisons t-test). (B) STM14028 count in ileo-cecal lymph nodes (ICLN), spleen, colon, and cecum of infected piglets. Data represent mean with error bars as SEM of six piglets per group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585093&req=5

Figure 1: STM14028 infection induces an increase of innate immunity cell compartment and colonizes different organs of piglets orally infected. (A) The prevalence of CD14+, CD21+, and polymorphonuclear (PMN) cells was determined in ileo-cecal lymph nodes, 48 h post-infection with STM14028. The differences were statistically significant (***P ≤ 0.001, multiple comparisons t-test). (B) STM14028 count in ileo-cecal lymph nodes (ICLN), spleen, colon, and cecum of infected piglets. Data represent mean with error bars as SEM of six piglets per group.
Mentions: In order to verify the involvement of the innate immune response during Salmonella infection in piglets, phenotypic analysis in ileo-cecal lymph nodes was performed in piglets orally infected with STM14028 and euthanized 48 h later. As depicted in Figure 1A, it was possible to observe a higher increase in the percentage of CD14+ (mainly monocytes) and polymorphonuclear cells with a minor involvement of CD21+ B cells. Moreover, the STM14028-infection was confirmed by the bacterial count in different organs (Figure 1B). These findings are consistent with a rapid recruitment of neutrophils and monocytes/macrophages toward lymph nodes, crucial for the effective response to lipopolysaccharides stimulus or concomitant bacterial infection. These data suggest a prompt involvement of the innate immune response in the regional lymph nodes draining the gut, after an oral infection with STM14028.

Bottom Line: This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment.This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization.Typhimurium exploits inflammation for its benefit in piglets.

View Article: PubMed Central - PubMed

Affiliation: Unit of Prophyilaxis and Control of Bacterial Zoonoses, Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità Rome, Italy.

ABSTRACT
Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization. Moreover, LPS in vivo treatment increased cytokines blood level and body temperature at 4 h post infection, which is consistent with an acute inflammatory stimulus, capable to influence the colonization of STM14028 in different organs and tissues. The present study proves for the first time that in acute enteric salmonellosis, S. Typhimurium exploits inflammation for its benefit in piglets.

No MeSH data available.


Related in: MedlinePlus