Limits...
The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp. Y35.

Li Y, Zhu H, Lei X, Zhang H, Cai G, Chen Z, Fu L, Xu H, Zheng T - Front Microbiol (2015)

Bottom Line: Harmful algal blooms (HABs) cause a variety of deleterious effects on aquatic ecosystems, especially the toxic dinoflagellate Alexandrium tamarense, which poses a serious threat to marine economic and human health based on releasing paralytic shellfish poison into the environment.Photosynthetic pigments including chlorophyll a and carotenoid decreased along with the photosynthetic efficiency being significantly inhibited.More than, the destruction of cell nuclear structure and inhibition of proliferating cell nuclear antigen (PCNA) related gene expression were confirmed.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University Xiamen, China ; College of Life Sciences, Henan Normal University Xinxiang, China.

ABSTRACT
Harmful algal blooms (HABs) cause a variety of deleterious effects on aquatic ecosystems, especially the toxic dinoflagellate Alexandrium tamarense, which poses a serious threat to marine economic and human health based on releasing paralytic shellfish poison into the environment. The algicidal bacterium Deinococcus sp. Y35 which can induce growth inhibition on A. tamarense was used to investigate the functional mechanism. The growth status, reactive oxygen species (ROS) content, photosynthetic system and the nuclear system of algal cells were determined under algicidal activity. A culture of strain Y35 not only induced overproduction of ROS in algal cells within only 0.5 h of treatment, also decrease the total protein content as well as the response of the antioxidant enzyme. Meanwhile, lipid peroxidation was induced and cell membrane integrity was lost. Photosynthetic pigments including chlorophyll a and carotenoid decreased along with the photosynthetic efficiency being significantly inhibited. At the same time, photosynthesis-related gene expression showed down-regulation. More than, the destruction of cell nuclear structure and inhibition of proliferating cell nuclear antigen (PCNA) related gene expression were confirmed. The potential functional mechanism of the algicidal bacterium on A. tamarense was investigated and provided a novel viewpoint which could be used in HABs control.

No MeSH data available.


Related in: MedlinePlus

The algal fluorescence intensity and algal culture of strain Y35 at concentrations of 0.5, 1.0, 2.0, and 3.0% on A. tamarense at different time points. All error bars indicate the SE of the three biological replicates.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585090&req=5

Figure 1: The algal fluorescence intensity and algal culture of strain Y35 at concentrations of 0.5, 1.0, 2.0, and 3.0% on A. tamarense at different time points. All error bars indicate the SE of the three biological replicates.

Mentions: The algicidal activity of the different concentrations (0.5, 1.0, 2.0, and 3.0%) of strain Y35 cultures was reflected in the fluorescence intensity of the algal cultures (Figure 1). We also compared the fluorescence intensity in the control group with added LB or sterile f/2 medium and the normal algal culture, and there were no obvious differences between them. Therefore, we used the control group with LB medium added as the experimental control group. The fluorescence intensity of algal cultures with concentrations of 1.0, 2.0, and 3.0% showed a significant decrease compared to the control group, but concentration of 0.5% did not exhibit clear differences with the control until 36 h of treatment. Within 48 h of treatment, the algicidal rate of the treatment groups with concentrations of 1.0, 2.0, and 3.0% reached 45, 85, and 87%. Most of the algal cells lysed and died after adding the bacterial culture for 24 h, and the algal culture faded to pale or white color. At the same time, numbers of intact cells were present in the control and the 0.5% concentration treatment group, and the color of the algal culture was brown and normal.


The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp. Y35.

Li Y, Zhu H, Lei X, Zhang H, Cai G, Chen Z, Fu L, Xu H, Zheng T - Front Microbiol (2015)

The algal fluorescence intensity and algal culture of strain Y35 at concentrations of 0.5, 1.0, 2.0, and 3.0% on A. tamarense at different time points. All error bars indicate the SE of the three biological replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585090&req=5

Figure 1: The algal fluorescence intensity and algal culture of strain Y35 at concentrations of 0.5, 1.0, 2.0, and 3.0% on A. tamarense at different time points. All error bars indicate the SE of the three biological replicates.
Mentions: The algicidal activity of the different concentrations (0.5, 1.0, 2.0, and 3.0%) of strain Y35 cultures was reflected in the fluorescence intensity of the algal cultures (Figure 1). We also compared the fluorescence intensity in the control group with added LB or sterile f/2 medium and the normal algal culture, and there were no obvious differences between them. Therefore, we used the control group with LB medium added as the experimental control group. The fluorescence intensity of algal cultures with concentrations of 1.0, 2.0, and 3.0% showed a significant decrease compared to the control group, but concentration of 0.5% did not exhibit clear differences with the control until 36 h of treatment. Within 48 h of treatment, the algicidal rate of the treatment groups with concentrations of 1.0, 2.0, and 3.0% reached 45, 85, and 87%. Most of the algal cells lysed and died after adding the bacterial culture for 24 h, and the algal culture faded to pale or white color. At the same time, numbers of intact cells were present in the control and the 0.5% concentration treatment group, and the color of the algal culture was brown and normal.

Bottom Line: Harmful algal blooms (HABs) cause a variety of deleterious effects on aquatic ecosystems, especially the toxic dinoflagellate Alexandrium tamarense, which poses a serious threat to marine economic and human health based on releasing paralytic shellfish poison into the environment.Photosynthetic pigments including chlorophyll a and carotenoid decreased along with the photosynthetic efficiency being significantly inhibited.More than, the destruction of cell nuclear structure and inhibition of proliferating cell nuclear antigen (PCNA) related gene expression were confirmed.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University Xiamen, China ; College of Life Sciences, Henan Normal University Xinxiang, China.

ABSTRACT
Harmful algal blooms (HABs) cause a variety of deleterious effects on aquatic ecosystems, especially the toxic dinoflagellate Alexandrium tamarense, which poses a serious threat to marine economic and human health based on releasing paralytic shellfish poison into the environment. The algicidal bacterium Deinococcus sp. Y35 which can induce growth inhibition on A. tamarense was used to investigate the functional mechanism. The growth status, reactive oxygen species (ROS) content, photosynthetic system and the nuclear system of algal cells were determined under algicidal activity. A culture of strain Y35 not only induced overproduction of ROS in algal cells within only 0.5 h of treatment, also decrease the total protein content as well as the response of the antioxidant enzyme. Meanwhile, lipid peroxidation was induced and cell membrane integrity was lost. Photosynthetic pigments including chlorophyll a and carotenoid decreased along with the photosynthetic efficiency being significantly inhibited. At the same time, photosynthesis-related gene expression showed down-regulation. More than, the destruction of cell nuclear structure and inhibition of proliferating cell nuclear antigen (PCNA) related gene expression were confirmed. The potential functional mechanism of the algicidal bacterium on A. tamarense was investigated and provided a novel viewpoint which could be used in HABs control.

No MeSH data available.


Related in: MedlinePlus