Limits...
Analysis of the protein related receptor GPR92 in G-cells.

Rettenberger AT, Schulze W, Breer H, Haid D - Front Physiol (2015)

Bottom Line: Our previous studies have shown that G-cells express suitable receptor types, most notably the peptone-receptor GPR92 and the amino acid receptors GPRC6A and CaSR; however, their relative importance remained unclear.The results indicate that the relative amount of receptor protein for GPR92 was much higher than for the receptor types GPRC6A and CaSR.These findings support the notion that the peptone-receptor GPR92 may be particularly relevant for sensing partially digested protein products.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, University of Hohenheim Stuttgart, Germany.

ABSTRACT
A continuous assessment of ingested food in the gastric lumen is essential for fine-tuning the digestive activities, including the secretion of the regulatory hormones such as gastrin. It has been proposed that G-cells may be able to sense the amount of ingested proteins and adjust the secretion of gastrin accordingly. Our previous studies have shown that G-cells express suitable receptor types, most notably the peptone-receptor GPR92 and the amino acid receptors GPRC6A and CaSR; however, their relative importance remained unclear. To determine the relative quantity of each receptor type, individual G-cells isolated from the transgenic mouse line mGas-EGFP were analyzed by means of a Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) procedure. The results indicate that the relative amount of receptor protein for GPR92 was much higher than for the receptor types GPRC6A and CaSR. These findings support the notion that the peptone-receptor GPR92 may be particularly relevant for sensing partially digested protein products. This view was supported by the finding that a high-protein diet affected the expression level of the peptone-receptor GPR92 in the gastric antrum as well as in the circumvallate papillae.

No MeSH data available.


Related in: MedlinePlus

Label-free relative quantitation of receptor proteins in isolated G-cells from normal fed mGas-EGFP mice. Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analyses were performed to determine the relative abundance of receptor proteins in isolated G-cells from one mouse investigated as triplicate. Each run contained 100 G-cells. The results indicate a high abundance of GPR92 receptor protein, whereas GPRC6A and especially CaSR receptor protein levels were rather low. Data are expressed as mean iBAQ scores ± SD (*P ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585063&req=5

Figure 3: Label-free relative quantitation of receptor proteins in isolated G-cells from normal fed mGas-EGFP mice. Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analyses were performed to determine the relative abundance of receptor proteins in isolated G-cells from one mouse investigated as triplicate. Each run contained 100 G-cells. The results indicate a high abundance of GPR92 receptor protein, whereas GPRC6A and especially CaSR receptor protein levels were rather low. Data are expressed as mean iBAQ scores ± SD (*P ≤ 0.05).

Mentions: The relative amount of proteins in isolated G-cells was determined in a label-free relative quantitation by means of Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS). Three replicate runs of protein extracted from 100 isolated cells have led to the identification of 416 proteins. Three receptors GPR92 (three peptides), GPCR6A (six peptides) and CaSR (three peptides) were unambiguously identified with at least three tryptic peptides. For CaSR one, and for GPR92 and GPRC6A two of these peptides were identified in each of the replicates, supported by other peptides that were identified in one or two replicates only (Supplementary Table 2). Sequence coverage was 2.7% for CaSR, 4% for GPRC6A, and 3.6% for GPR92. The quantitative results of this approach are depicted in Figure 3. Databased on the iBAQ abundance values, GPR92 was found as the receptor with the highest abundance while only moderate levels were observed for GPRC6A and CaSR. This finding suggests a prominent role of GPR92 in G-cells.


Analysis of the protein related receptor GPR92 in G-cells.

Rettenberger AT, Schulze W, Breer H, Haid D - Front Physiol (2015)

Label-free relative quantitation of receptor proteins in isolated G-cells from normal fed mGas-EGFP mice. Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analyses were performed to determine the relative abundance of receptor proteins in isolated G-cells from one mouse investigated as triplicate. Each run contained 100 G-cells. The results indicate a high abundance of GPR92 receptor protein, whereas GPRC6A and especially CaSR receptor protein levels were rather low. Data are expressed as mean iBAQ scores ± SD (*P ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585063&req=5

Figure 3: Label-free relative quantitation of receptor proteins in isolated G-cells from normal fed mGas-EGFP mice. Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analyses were performed to determine the relative abundance of receptor proteins in isolated G-cells from one mouse investigated as triplicate. Each run contained 100 G-cells. The results indicate a high abundance of GPR92 receptor protein, whereas GPRC6A and especially CaSR receptor protein levels were rather low. Data are expressed as mean iBAQ scores ± SD (*P ≤ 0.05).
Mentions: The relative amount of proteins in isolated G-cells was determined in a label-free relative quantitation by means of Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS). Three replicate runs of protein extracted from 100 isolated cells have led to the identification of 416 proteins. Three receptors GPR92 (three peptides), GPCR6A (six peptides) and CaSR (three peptides) were unambiguously identified with at least three tryptic peptides. For CaSR one, and for GPR92 and GPRC6A two of these peptides were identified in each of the replicates, supported by other peptides that were identified in one or two replicates only (Supplementary Table 2). Sequence coverage was 2.7% for CaSR, 4% for GPRC6A, and 3.6% for GPR92. The quantitative results of this approach are depicted in Figure 3. Databased on the iBAQ abundance values, GPR92 was found as the receptor with the highest abundance while only moderate levels were observed for GPRC6A and CaSR. This finding suggests a prominent role of GPR92 in G-cells.

Bottom Line: Our previous studies have shown that G-cells express suitable receptor types, most notably the peptone-receptor GPR92 and the amino acid receptors GPRC6A and CaSR; however, their relative importance remained unclear.The results indicate that the relative amount of receptor protein for GPR92 was much higher than for the receptor types GPRC6A and CaSR.These findings support the notion that the peptone-receptor GPR92 may be particularly relevant for sensing partially digested protein products.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, University of Hohenheim Stuttgart, Germany.

ABSTRACT
A continuous assessment of ingested food in the gastric lumen is essential for fine-tuning the digestive activities, including the secretion of the regulatory hormones such as gastrin. It has been proposed that G-cells may be able to sense the amount of ingested proteins and adjust the secretion of gastrin accordingly. Our previous studies have shown that G-cells express suitable receptor types, most notably the peptone-receptor GPR92 and the amino acid receptors GPRC6A and CaSR; however, their relative importance remained unclear. To determine the relative quantity of each receptor type, individual G-cells isolated from the transgenic mouse line mGas-EGFP were analyzed by means of a Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) procedure. The results indicate that the relative amount of receptor protein for GPR92 was much higher than for the receptor types GPRC6A and CaSR. These findings support the notion that the peptone-receptor GPR92 may be particularly relevant for sensing partially digested protein products. This view was supported by the finding that a high-protein diet affected the expression level of the peptone-receptor GPR92 in the gastric antrum as well as in the circumvallate papillae.

No MeSH data available.


Related in: MedlinePlus