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Enhanced functional connectivity involving the ventromedial hypothalamus following methamphetamine exposure.

Zuloaga DG, Iancu OD, Weber S, Etzel D, Marzulla T, Stewart B, Allen CN, Raber J - Front Neurosci (2015)

Bottom Line: MA phase shifts, entrains the circadian clock and can also alter the entraining effect of light by currently unknown mechanisms.There were five distinct patterns of neuronal activation.Functional connectivity between the ventromedial hypothalamus (VMH) and other brain areas, including the paraventricular nucleus of the hypothalamus and basolateral and medial amygdala, was enhanced following MA exposure, suggesting a role for the VMH in the effects of MA on the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University Portland Portland, OR, USA ; Department of Psychology, University at Albany Albany, NY, USA.

ABSTRACT
Methamphetamine (MA) consumption causes disruption of many biological rhythms including the sleep-wake cycle. This circadian effect is seen shortly following MA exposure and later in life following developmental MA exposure. MA phase shifts, entrains the circadian clock and can also alter the entraining effect of light by currently unknown mechanisms. We analyzed and compared immunoreactivity of the immediate early gene c-Fos, a marker of neuronal activity, to assess neuronal activation 2 h following MA exposure in the light and dark phases. We used network analyses of correlation patterns derived from global brain immunoreactivity patterns of c-Fos, to infer functional connectivity between brain regions. There were five distinct patterns of neuronal activation. In several brain areas, neuronal activation following exposure to MA was stronger in the light than the dark phase, highlighting the importance of considering circadian periods of increased effects of MA in defining experimental conditions and understanding the mechanisms underlying detrimental effects of MA exposure to brain function. Functional connectivity between the ventromedial hypothalamus (VMH) and other brain areas, including the paraventricular nucleus of the hypothalamus and basolateral and medial amygdala, was enhanced following MA exposure, suggesting a role for the VMH in the effects of MA on the brain.

No MeSH data available.


Related in: MedlinePlus

(A) Similar c-Fos/AVP dual-labeling in the PVN following MA exposure in the AM and PM. Mice treated with MA in the day and night showed no increase in c-Fos/AVP-ir cells in the PVN compared to saline-treated mice. n = 10 mice/treatment/time period. (B) Representative panel of c-Fos (red) and AVP (green) from a mouse treated with MA in the AM is shown.
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Figure 6: (A) Similar c-Fos/AVP dual-labeling in the PVN following MA exposure in the AM and PM. Mice treated with MA in the day and night showed no increase in c-Fos/AVP-ir cells in the PVN compared to saline-treated mice. n = 10 mice/treatment/time period. (B) Representative panel of c-Fos (red) and AVP (green) from a mouse treated with MA in the AM is shown.

Mentions: Analysis of the number of c-Fos/AVP dual labeled cells in the PVN revealed comparable numbers in all four experimental groups and no effect of MA treatment (Figure 6A; see Figure 6B for a representative image of c-Fos/AVP double labeling). There was no effect of MA treatment on the number of c-Fos/AVP dual labeled cells in the SCN either [F(1, 12) = 0.16, p = 0.69, data not shown].


Enhanced functional connectivity involving the ventromedial hypothalamus following methamphetamine exposure.

Zuloaga DG, Iancu OD, Weber S, Etzel D, Marzulla T, Stewart B, Allen CN, Raber J - Front Neurosci (2015)

(A) Similar c-Fos/AVP dual-labeling in the PVN following MA exposure in the AM and PM. Mice treated with MA in the day and night showed no increase in c-Fos/AVP-ir cells in the PVN compared to saline-treated mice. n = 10 mice/treatment/time period. (B) Representative panel of c-Fos (red) and AVP (green) from a mouse treated with MA in the AM is shown.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4585047&req=5

Figure 6: (A) Similar c-Fos/AVP dual-labeling in the PVN following MA exposure in the AM and PM. Mice treated with MA in the day and night showed no increase in c-Fos/AVP-ir cells in the PVN compared to saline-treated mice. n = 10 mice/treatment/time period. (B) Representative panel of c-Fos (red) and AVP (green) from a mouse treated with MA in the AM is shown.
Mentions: Analysis of the number of c-Fos/AVP dual labeled cells in the PVN revealed comparable numbers in all four experimental groups and no effect of MA treatment (Figure 6A; see Figure 6B for a representative image of c-Fos/AVP double labeling). There was no effect of MA treatment on the number of c-Fos/AVP dual labeled cells in the SCN either [F(1, 12) = 0.16, p = 0.69, data not shown].

Bottom Line: MA phase shifts, entrains the circadian clock and can also alter the entraining effect of light by currently unknown mechanisms.There were five distinct patterns of neuronal activation.Functional connectivity between the ventromedial hypothalamus (VMH) and other brain areas, including the paraventricular nucleus of the hypothalamus and basolateral and medial amygdala, was enhanced following MA exposure, suggesting a role for the VMH in the effects of MA on the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University Portland Portland, OR, USA ; Department of Psychology, University at Albany Albany, NY, USA.

ABSTRACT
Methamphetamine (MA) consumption causes disruption of many biological rhythms including the sleep-wake cycle. This circadian effect is seen shortly following MA exposure and later in life following developmental MA exposure. MA phase shifts, entrains the circadian clock and can also alter the entraining effect of light by currently unknown mechanisms. We analyzed and compared immunoreactivity of the immediate early gene c-Fos, a marker of neuronal activity, to assess neuronal activation 2 h following MA exposure in the light and dark phases. We used network analyses of correlation patterns derived from global brain immunoreactivity patterns of c-Fos, to infer functional connectivity between brain regions. There were five distinct patterns of neuronal activation. In several brain areas, neuronal activation following exposure to MA was stronger in the light than the dark phase, highlighting the importance of considering circadian periods of increased effects of MA in defining experimental conditions and understanding the mechanisms underlying detrimental effects of MA exposure to brain function. Functional connectivity between the ventromedial hypothalamus (VMH) and other brain areas, including the paraventricular nucleus of the hypothalamus and basolateral and medial amygdala, was enhanced following MA exposure, suggesting a role for the VMH in the effects of MA on the brain.

No MeSH data available.


Related in: MedlinePlus