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French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.


Correlation between host genetic richness, host genetic diversity, and midgut bacterial diversity. The mean bacterial Shannon α-diversity (H′) was correlated with (A) rarefied genetic richness, Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) and (B) diversity, Hs (Spearman's rank correlation, ρ = 0.78, p = 0.048). (F), France; (V), Vietnam; NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng; BGM, Bù Gia Mâp. Standard deviation of Shannon α-diversity (H′) was represented for each site.
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Figure 6: Correlation between host genetic richness, host genetic diversity, and midgut bacterial diversity. The mean bacterial Shannon α-diversity (H′) was correlated with (A) rarefied genetic richness, Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) and (B) diversity, Hs (Spearman's rank correlation, ρ = 0.78, p = 0.048). (F), France; (V), Vietnam; NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng; BGM, Bù Gia Mâp. Standard deviation of Shannon α-diversity (H′) was represented for each site.

Mentions: The populations sampled were compared to assess whether there was any relationship between the bacterial diversity and the genetic diversity of the mosquitoes. Comparative analysis showed a low correlation between bacterial β-diversity (Bray-Curtis dissimilarity distance) and haplotype structure (Mantel, R2 = 0.5, p = 0.02) and no significant correlation with the Cavalli-Sforza Edwards measure of microsatellite genetic distance (Mantel, R2 = 0.20, p = 0.19). However, for all sampling sites, positive correlations were observed between mean bacterial α-diversity (H′) and respectively host Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) (Figure 6A) and host genetic diversity (Hs) (Spearman's rank correlation, ρ = 0.78, p = 0.048) (Figure 6B).


French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Correlation between host genetic richness, host genetic diversity, and midgut bacterial diversity. The mean bacterial Shannon α-diversity (H′) was correlated with (A) rarefied genetic richness, Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) and (B) diversity, Hs (Spearman's rank correlation, ρ = 0.78, p = 0.048). (F), France; (V), Vietnam; NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng; BGM, Bù Gia Mâp. Standard deviation of Shannon α-diversity (H′) was represented for each site.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585046&req=5

Figure 6: Correlation between host genetic richness, host genetic diversity, and midgut bacterial diversity. The mean bacterial Shannon α-diversity (H′) was correlated with (A) rarefied genetic richness, Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) and (B) diversity, Hs (Spearman's rank correlation, ρ = 0.78, p = 0.048). (F), France; (V), Vietnam; NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng; BGM, Bù Gia Mâp. Standard deviation of Shannon α-diversity (H′) was represented for each site.
Mentions: The populations sampled were compared to assess whether there was any relationship between the bacterial diversity and the genetic diversity of the mosquitoes. Comparative analysis showed a low correlation between bacterial β-diversity (Bray-Curtis dissimilarity distance) and haplotype structure (Mantel, R2 = 0.5, p = 0.02) and no significant correlation with the Cavalli-Sforza Edwards measure of microsatellite genetic distance (Mantel, R2 = 0.20, p = 0.19). However, for all sampling sites, positive correlations were observed between mean bacterial α-diversity (H′) and respectively host Ar (Spearman's rank correlation, ρ = 0.95, p = 8.10−3) (Figure 6A) and host genetic diversity (Hs) (Spearman's rank correlation, ρ = 0.78, p = 0.048) (Figure 6B).

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.