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French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.


Related in: MedlinePlus

Aedes albopictus microbiota bacterial diversity. The heatmap represents the relative abundance of 3% OTUs in each sample. The Color Key Value refers to the proportion of an OTU in a single sample. The OTUs were represented if their proportion was =0.01 in at least one sample. They were named by their taxonomic assignment according to Bayesian classification. When more than one OTU get the same assignation, they were differentiated with numbers. Samples are classified according to their own populations. Individual midgut samples from Vietnam were collected in Bình Du'o'ng (BD 3, 4, 5, 6, and 7), Bù Gia Mâp (BGM 1, 3, 4), Hồ Chí Minh City (HCM 6, 7, 8, 9, 10), Vung Tàu City (VT 3, 4, 9, 10, 11). Individual midgut samples from France were collected in Nice (NC 5, 6, 12, 17), Portes-Lès-Valence (PLV 3, 4, 7, 8, 10), and Saint Priest (SP 1, 2, 5, 6, 7). The pooled carcasses of the following individuals were also analyzed for both Vietnamese (BDC, BGMC, HCMC, VTC) and French populations (NCC, PLVC, SPC); the last “C” added to each name indicates carcass.
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Figure 3: Aedes albopictus microbiota bacterial diversity. The heatmap represents the relative abundance of 3% OTUs in each sample. The Color Key Value refers to the proportion of an OTU in a single sample. The OTUs were represented if their proportion was =0.01 in at least one sample. They were named by their taxonomic assignment according to Bayesian classification. When more than one OTU get the same assignation, they were differentiated with numbers. Samples are classified according to their own populations. Individual midgut samples from Vietnam were collected in Bình Du'o'ng (BD 3, 4, 5, 6, and 7), Bù Gia Mâp (BGM 1, 3, 4), Hồ Chí Minh City (HCM 6, 7, 8, 9, 10), Vung Tàu City (VT 3, 4, 9, 10, 11). Individual midgut samples from France were collected in Nice (NC 5, 6, 12, 17), Portes-Lès-Valence (PLV 3, 4, 7, 8, 10), and Saint Priest (SP 1, 2, 5, 6, 7). The pooled carcasses of the following individuals were also analyzed for both Vietnamese (BDC, BGMC, HCMC, VTC) and French populations (NCC, PLVC, SPC); the last “C” added to each name indicates carcass.

Mentions: Our aim was to study the bacterial microbiota in autochthonous and invasive tiger mosquito populations. In a previous study, we demonstrated that Wolbachia is the predominant bacterial species in Ae. albopictus from Madagascar when using whole body genomic DNA, constituting up to 99% of high throughput sequences recovered (Minard et al., 2014). Here, we first tested whether the two Wolbachia strains wAlbA and wAlbB were also present and dominant in mosquitoes sampled from autochthonous populations at four sites (HCM, BD, VT, BGM) in Vietnam and from invasive populations at three sites (SP, PLV, NC) in France (Figure 1). wAlbA and wAlbB were detected in all individuals of the seven populations. The lowest densities of wAlbA and wAlbB strains detected were 5.25 × 10−5 wsp.actin−1 and 3.09 × 10−3 wsp.actin−1 copies respectively in mosquito samples from BD and the highest densities of 2.44 wsp.actin−1 and 8.53 wsp.actin−1 copies respectively from the BGM population (Figure 2). A significant positive correlation (Pearson's correlation R2 = 0.84, p < 2.2 × 10−16) between wAlbA and wAlbB strains was found among all the populations tested (Figure 2). To check if Wolbachia sequences were overrepresented in bacterial microbiota sequences when applying NGS methods to the whole body, the V5-V6 rrs amplicons were generated from mosquito carcass pools as indicated in material and methods, and sequenced by Miseq technology. Results confirmed a dominance of Wolbachia OTUs (Figure 3) which account for 28% (for HCM) to 91% (for SP) of the sequence dataset, reinforcing the rationale for our choice to avoid using the whole insect body for bacterial community analysis in Ae. albopictus.


French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Aedes albopictus microbiota bacterial diversity. The heatmap represents the relative abundance of 3% OTUs in each sample. The Color Key Value refers to the proportion of an OTU in a single sample. The OTUs were represented if their proportion was =0.01 in at least one sample. They were named by their taxonomic assignment according to Bayesian classification. When more than one OTU get the same assignation, they were differentiated with numbers. Samples are classified according to their own populations. Individual midgut samples from Vietnam were collected in Bình Du'o'ng (BD 3, 4, 5, 6, and 7), Bù Gia Mâp (BGM 1, 3, 4), Hồ Chí Minh City (HCM 6, 7, 8, 9, 10), Vung Tàu City (VT 3, 4, 9, 10, 11). Individual midgut samples from France were collected in Nice (NC 5, 6, 12, 17), Portes-Lès-Valence (PLV 3, 4, 7, 8, 10), and Saint Priest (SP 1, 2, 5, 6, 7). The pooled carcasses of the following individuals were also analyzed for both Vietnamese (BDC, BGMC, HCMC, VTC) and French populations (NCC, PLVC, SPC); the last “C” added to each name indicates carcass.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585046&req=5

Figure 3: Aedes albopictus microbiota bacterial diversity. The heatmap represents the relative abundance of 3% OTUs in each sample. The Color Key Value refers to the proportion of an OTU in a single sample. The OTUs were represented if their proportion was =0.01 in at least one sample. They were named by their taxonomic assignment according to Bayesian classification. When more than one OTU get the same assignation, they were differentiated with numbers. Samples are classified according to their own populations. Individual midgut samples from Vietnam were collected in Bình Du'o'ng (BD 3, 4, 5, 6, and 7), Bù Gia Mâp (BGM 1, 3, 4), Hồ Chí Minh City (HCM 6, 7, 8, 9, 10), Vung Tàu City (VT 3, 4, 9, 10, 11). Individual midgut samples from France were collected in Nice (NC 5, 6, 12, 17), Portes-Lès-Valence (PLV 3, 4, 7, 8, 10), and Saint Priest (SP 1, 2, 5, 6, 7). The pooled carcasses of the following individuals were also analyzed for both Vietnamese (BDC, BGMC, HCMC, VTC) and French populations (NCC, PLVC, SPC); the last “C” added to each name indicates carcass.
Mentions: Our aim was to study the bacterial microbiota in autochthonous and invasive tiger mosquito populations. In a previous study, we demonstrated that Wolbachia is the predominant bacterial species in Ae. albopictus from Madagascar when using whole body genomic DNA, constituting up to 99% of high throughput sequences recovered (Minard et al., 2014). Here, we first tested whether the two Wolbachia strains wAlbA and wAlbB were also present and dominant in mosquitoes sampled from autochthonous populations at four sites (HCM, BD, VT, BGM) in Vietnam and from invasive populations at three sites (SP, PLV, NC) in France (Figure 1). wAlbA and wAlbB were detected in all individuals of the seven populations. The lowest densities of wAlbA and wAlbB strains detected were 5.25 × 10−5 wsp.actin−1 and 3.09 × 10−3 wsp.actin−1 copies respectively in mosquito samples from BD and the highest densities of 2.44 wsp.actin−1 and 8.53 wsp.actin−1 copies respectively from the BGM population (Figure 2). A significant positive correlation (Pearson's correlation R2 = 0.84, p < 2.2 × 10−16) between wAlbA and wAlbB strains was found among all the populations tested (Figure 2). To check if Wolbachia sequences were overrepresented in bacterial microbiota sequences when applying NGS methods to the whole body, the V5-V6 rrs amplicons were generated from mosquito carcass pools as indicated in material and methods, and sequenced by Miseq technology. Results confirmed a dominance of Wolbachia OTUs (Figure 3) which account for 28% (for HCM) to 91% (for SP) of the sequence dataset, reinforcing the rationale for our choice to avoid using the whole insect body for bacterial community analysis in Ae. albopictus.

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.


Related in: MedlinePlus