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French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.


Maps of sampling sites. Sampling sites and GPS coordinates (World Geodetic System 1984) in France (A) and Vietnam (B). NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng, BGM, Bù Gia Mâp. Scale bars, 200 km.
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Figure 1: Maps of sampling sites. Sampling sites and GPS coordinates (World Geodetic System 1984) in France (A) and Vietnam (B). NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng, BGM, Bù Gia Mâp. Scale bars, 200 km.

Mentions: Ae. albopictus specimens were sampled in Metropolitan France and Vietnam. In addition to their contrasted climate and ecology, these two countries were chosen as Vietnam is located in the South East Asia, the region where Ae. albopictus originated indicating ancient colonization, whereas Metropolitan France is a newly invaded zone. Sampling in Metropolitan France was performed between August and September 2012 at Saint-Priest (SP), Portes-Lès-Valence (PLV), and Nice (NC). NC is one of the first invaded sites in France since 2004 (Medlock et al., 2012), whereas PLV and SP were colonized in 2011 and 2012, respectively (data obtained from French health organization INVS). Mosquito sampling in Vietnam was performed during October 2012 at Hồ Chí Minh City (HCM), Bình Du'o'ng (BD), Vung Tàu City (VT), and Bù Gia Mâp (BGM) (Figure 1). All sites were urban or suburban, except BGM located in a protected forest national park. Sampling sites were at least 18 km from each other to avoid sampling populations originated from the same breeding site. Consequently, we assume that individuals collected from the same sites belong to the same population as they share breeding sites, and a total of seven independent populations (three from Metropolitan France and four from Vietnam) were obtained and analyzed. Live adult females were caught with nets or BG-Traps and then identified using morphological characteristics (Rueda, 2004). For some individuals, identification was confirmed by COI barcode sequencing (see below). To control confounding effects from nutritional factors, only females that could be seen to contain no blood upon magnified observation of the gut contents were retained for analysis. Mosquitoes were stored in 100% ethanol at −80°C until used.


French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives.

Minard G, Tran FH, Van VT, Goubert C, Bellet C, Lambert G, Kim KL, Thuy TH, Mavingui P, Valiente Moro C - Front Microbiol (2015)

Maps of sampling sites. Sampling sites and GPS coordinates (World Geodetic System 1984) in France (A) and Vietnam (B). NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng, BGM, Bù Gia Mâp. Scale bars, 200 km.
© Copyright Policy
Related In: Results  -  Collection

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Figure 1: Maps of sampling sites. Sampling sites and GPS coordinates (World Geodetic System 1984) in France (A) and Vietnam (B). NC, Nice; PLV, Porte-lès-Valence; SP, Saint Priest; VT, Vung Tàu City; HCM, Hồ Chí Minh City; BD, Bình Du'o'ng, BGM, Bù Gia Mâp. Scale bars, 200 km.
Mentions: Ae. albopictus specimens were sampled in Metropolitan France and Vietnam. In addition to their contrasted climate and ecology, these two countries were chosen as Vietnam is located in the South East Asia, the region where Ae. albopictus originated indicating ancient colonization, whereas Metropolitan France is a newly invaded zone. Sampling in Metropolitan France was performed between August and September 2012 at Saint-Priest (SP), Portes-Lès-Valence (PLV), and Nice (NC). NC is one of the first invaded sites in France since 2004 (Medlock et al., 2012), whereas PLV and SP were colonized in 2011 and 2012, respectively (data obtained from French health organization INVS). Mosquito sampling in Vietnam was performed during October 2012 at Hồ Chí Minh City (HCM), Bình Du'o'ng (BD), Vung Tàu City (VT), and Bù Gia Mâp (BGM) (Figure 1). All sites were urban or suburban, except BGM located in a protected forest national park. Sampling sites were at least 18 km from each other to avoid sampling populations originated from the same breeding site. Consequently, we assume that individuals collected from the same sites belong to the same population as they share breeding sites, and a total of seven independent populations (three from Metropolitan France and four from Vietnam) were obtained and analyzed. Live adult females were caught with nets or BG-Traps and then identified using morphological characteristics (Rueda, 2004). For some individuals, identification was confirmed by COI barcode sequencing (see below). To control confounding effects from nutritional factors, only females that could be seen to contain no blood upon magnified observation of the gut contents were retained for analysis. Mosquitoes were stored in 100% ethanol at −80°C until used.

Bottom Line: The insect-associated microbiota is now recognized to play a significant role in host biology.Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam.These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

View Article: PubMed Central - PubMed

Affiliation: Ecologie Microbienne, UMR Centre National de la Recherche Scientifique 5557, USC INRA 1364, VetAgro Sup, FR41 BioEnvironment and Health, Université Claude Bernard Lyon 1 Villeurbanne, France.

ABSTRACT
The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.

No MeSH data available.