Limits...
Organogel-nanoemulsion containing nisin and D-limonene and its antimicrobial activity.

Bei W, Zhou Y, Xing X, Zahi MR, Li Y, Yuan Q, Liang H - Front Microbiol (2015)

Bottom Line: The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity.The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR.Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.

ABSTRACT
The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with D-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio (SOR) were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with D-limonene and nisin were confirmed by minimal inhibitory concentrations comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents' release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.

No MeSH data available.


Related in: MedlinePlus

Growth curves of Escherichia coli(A), Staphylococcus aureus(B), and Bacillus subtilis(C) treated with different concentrations [control, minimal inhibitory concentrations (MIC), 2 × MIC, 4 × MIC] of organogel-nanoemulsion containing D-limonene and nisin.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4585035&req=5

Figure 4: Growth curves of Escherichia coli(A), Staphylococcus aureus(B), and Bacillus subtilis(C) treated with different concentrations [control, minimal inhibitory concentrations (MIC), 2 × MIC, 4 × MIC] of organogel-nanoemulsion containing D-limonene and nisin.

Mentions: In order to further verify the antibacterial effects of organogel-nanoemulsions growth curve of three target microorganisms were detected under different conditions in this study. The strains were treated with 0, MIC, 2 × MIC and 4 × MIC of organogel-nanoemulsions containing D-limonene (15% w/w) and nisin (6% w/w). After incubation for 168 h, the growth cures were summarized in Figure 4. As shown in Figure 4, organogel-nanoemulsions containing D-limonene and nisin displayed significant inhibitory effects against all the target microorganisms, especially for E.coli which shown the best results (Figure 4A). Comparing with blank samples of uncontrolled growth, organogel-nanoemulsions containing D-limonene and nisin effectively inhibited the division and reproduction of logarithmic phase of bacterium. The number of living bacterium decreased obviously under the treatments of organogel-nanoemulsions. Furthermore, the growth corves under treatment couldn’t reach the normal growth peak any more but quickly enter into decline phase, especially for the 4 × MIC which shown basically no obvious growth in number of bacterium (Figure 4).


Organogel-nanoemulsion containing nisin and D-limonene and its antimicrobial activity.

Bei W, Zhou Y, Xing X, Zahi MR, Li Y, Yuan Q, Liang H - Front Microbiol (2015)

Growth curves of Escherichia coli(A), Staphylococcus aureus(B), and Bacillus subtilis(C) treated with different concentrations [control, minimal inhibitory concentrations (MIC), 2 × MIC, 4 × MIC] of organogel-nanoemulsion containing D-limonene and nisin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585035&req=5

Figure 4: Growth curves of Escherichia coli(A), Staphylococcus aureus(B), and Bacillus subtilis(C) treated with different concentrations [control, minimal inhibitory concentrations (MIC), 2 × MIC, 4 × MIC] of organogel-nanoemulsion containing D-limonene and nisin.
Mentions: In order to further verify the antibacterial effects of organogel-nanoemulsions growth curve of three target microorganisms were detected under different conditions in this study. The strains were treated with 0, MIC, 2 × MIC and 4 × MIC of organogel-nanoemulsions containing D-limonene (15% w/w) and nisin (6% w/w). After incubation for 168 h, the growth cures were summarized in Figure 4. As shown in Figure 4, organogel-nanoemulsions containing D-limonene and nisin displayed significant inhibitory effects against all the target microorganisms, especially for E.coli which shown the best results (Figure 4A). Comparing with blank samples of uncontrolled growth, organogel-nanoemulsions containing D-limonene and nisin effectively inhibited the division and reproduction of logarithmic phase of bacterium. The number of living bacterium decreased obviously under the treatments of organogel-nanoemulsions. Furthermore, the growth corves under treatment couldn’t reach the normal growth peak any more but quickly enter into decline phase, especially for the 4 × MIC which shown basically no obvious growth in number of bacterium (Figure 4).

Bottom Line: The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity.The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR.Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology Beijing, China.

ABSTRACT
The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with D-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio (SOR) were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low SOR. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with D-limonene and nisin were confirmed by minimal inhibitory concentrations comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents' release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.

No MeSH data available.


Related in: MedlinePlus