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Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance.

Kim YH, Hwang SJ, Waqas M, Khan AL, Lee JH, Lee JD, Nguyen HT, Lee IJ - Front Plant Sci (2015)

Bottom Line: Endogenous hormones also showed significant differences between WTL and WSL.The ratio of total GA and ABA was significantly higher in WTL than in WSL.Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biosciences, Kyungpook National University Daegu, South Korea ; Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri Columbia, MO, USA.

ABSTRACT
Waterlogged condition due to flooding is one of the major abiotic stresses that drastically affect the soybean growth and yield around the world. As a result, many breeders have focused on the development of waterlogging tolerance in soybean varieties, and thus, several tolerant varieties were developed. However, the physiological mechanism of waterlogging tolerance is not yet fully understood. We particularly studied the endogenous hormones regulation during waterlogging in two contrasting soybean genotypes. According to our results, adventitious roots were better developed in the waterlogging tolerant line (WTL) than in the waterlogging susceptible line (WSL). Endogenous hormones also showed significant differences between WTL and WSL. The ethylene production ratio was higher in WTL than in WSL, and methionine was higher in WTL than in WSL. Other endogenous abscisic acid (ABA) contents were lower in WTL than in WSL. Conversely, gibberellic acid (GA) showed a tendency to be high in WTL, especially the levels of the bioactive GA4. The ratio of total GA and ABA was significantly higher in WTL than in WSL. Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

No MeSH data available.


The scheme of the waterlogging tolerance mechanism under plant hormones level. In the figure, solid lines indicate induction or increase and dashed line arrows indicate putative response. The line with bars shows suppressed or inhibited.
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Figure 8: The scheme of the waterlogging tolerance mechanism under plant hormones level. In the figure, solid lines indicate induction or increase and dashed line arrows indicate putative response. The line with bars shows suppressed or inhibited.

Mentions: It is concluded that flooding severely affect normal functioning of plants. To withstand this abiotic stress, plants bring in physiological changes for adaptation as shown by the hormonal and amino acid regulation, better development of aerenchyma cells and adventitious roots, and lipid peroxidation for waterlogging tolerance regulated by complex genetic mechanism (Figure 8). However, the difference in genetic background of tolerance and susceptible lines vary this complex genetic mechanism, which may contribute to the level of tolerance mechanism. It was revealed that flooding tolerance or masking the effect of flooding could be associated with quantitative trait loci (QTL). For instance, Nguyen et al. (2012) determined that resistance to injury or yield loss due to flooding or water logging tolerance in RIL may be genetically controlled and confer by the presence of genes located on four chromosomes. Furthermore, these two soybean lines were used as material for root-related transcription factors associated with flooding tolerance (Valliyodan et al., 2014). According to Valliyodan et al. (2014), they identified some different gene expression patterns involved in ethylene biosynthesis pathway. Therefore, further investigation is recommended at molecular level to dig out the genetic components and expression profile on suggested QTL enriched-chromosomes that associated with waterlogging stress tolerance particularly through hormonal modulation to maximize the level of tolerance for future breeding of crops. This will also reveal that many QTLs or hormonal level differences contribute to waterlogging tolerance.


Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance.

Kim YH, Hwang SJ, Waqas M, Khan AL, Lee JH, Lee JD, Nguyen HT, Lee IJ - Front Plant Sci (2015)

The scheme of the waterlogging tolerance mechanism under plant hormones level. In the figure, solid lines indicate induction or increase and dashed line arrows indicate putative response. The line with bars shows suppressed or inhibited.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585003&req=5

Figure 8: The scheme of the waterlogging tolerance mechanism under plant hormones level. In the figure, solid lines indicate induction or increase and dashed line arrows indicate putative response. The line with bars shows suppressed or inhibited.
Mentions: It is concluded that flooding severely affect normal functioning of plants. To withstand this abiotic stress, plants bring in physiological changes for adaptation as shown by the hormonal and amino acid regulation, better development of aerenchyma cells and adventitious roots, and lipid peroxidation for waterlogging tolerance regulated by complex genetic mechanism (Figure 8). However, the difference in genetic background of tolerance and susceptible lines vary this complex genetic mechanism, which may contribute to the level of tolerance mechanism. It was revealed that flooding tolerance or masking the effect of flooding could be associated with quantitative trait loci (QTL). For instance, Nguyen et al. (2012) determined that resistance to injury or yield loss due to flooding or water logging tolerance in RIL may be genetically controlled and confer by the presence of genes located on four chromosomes. Furthermore, these two soybean lines were used as material for root-related transcription factors associated with flooding tolerance (Valliyodan et al., 2014). According to Valliyodan et al. (2014), they identified some different gene expression patterns involved in ethylene biosynthesis pathway. Therefore, further investigation is recommended at molecular level to dig out the genetic components and expression profile on suggested QTL enriched-chromosomes that associated with waterlogging stress tolerance particularly through hormonal modulation to maximize the level of tolerance for future breeding of crops. This will also reveal that many QTLs or hormonal level differences contribute to waterlogging tolerance.

Bottom Line: Endogenous hormones also showed significant differences between WTL and WSL.The ratio of total GA and ABA was significantly higher in WTL than in WSL.Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biosciences, Kyungpook National University Daegu, South Korea ; Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri Columbia, MO, USA.

ABSTRACT
Waterlogged condition due to flooding is one of the major abiotic stresses that drastically affect the soybean growth and yield around the world. As a result, many breeders have focused on the development of waterlogging tolerance in soybean varieties, and thus, several tolerant varieties were developed. However, the physiological mechanism of waterlogging tolerance is not yet fully understood. We particularly studied the endogenous hormones regulation during waterlogging in two contrasting soybean genotypes. According to our results, adventitious roots were better developed in the waterlogging tolerant line (WTL) than in the waterlogging susceptible line (WSL). Endogenous hormones also showed significant differences between WTL and WSL. The ethylene production ratio was higher in WTL than in WSL, and methionine was higher in WTL than in WSL. Other endogenous abscisic acid (ABA) contents were lower in WTL than in WSL. Conversely, gibberellic acid (GA) showed a tendency to be high in WTL, especially the levels of the bioactive GA4. The ratio of total GA and ABA was significantly higher in WTL than in WSL. Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

No MeSH data available.