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Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance.

Kim YH, Hwang SJ, Waqas M, Khan AL, Lee JH, Lee JD, Nguyen HT, Lee IJ - Front Plant Sci (2015)

Bottom Line: Endogenous hormones also showed significant differences between WTL and WSL.The ratio of total GA and ABA was significantly higher in WTL than in WSL.Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biosciences, Kyungpook National University Daegu, South Korea ; Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri Columbia, MO, USA.

ABSTRACT
Waterlogged condition due to flooding is one of the major abiotic stresses that drastically affect the soybean growth and yield around the world. As a result, many breeders have focused on the development of waterlogging tolerance in soybean varieties, and thus, several tolerant varieties were developed. However, the physiological mechanism of waterlogging tolerance is not yet fully understood. We particularly studied the endogenous hormones regulation during waterlogging in two contrasting soybean genotypes. According to our results, adventitious roots were better developed in the waterlogging tolerant line (WTL) than in the waterlogging susceptible line (WSL). Endogenous hormones also showed significant differences between WTL and WSL. The ethylene production ratio was higher in WTL than in WSL, and methionine was higher in WTL than in WSL. Other endogenous abscisic acid (ABA) contents were lower in WTL than in WSL. Conversely, gibberellic acid (GA) showed a tendency to be high in WTL, especially the levels of the bioactive GA4. The ratio of total GA and ABA was significantly higher in WTL than in WSL. Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

No MeSH data available.


The images of root section (A–D) and MDA (E) in root after 10 days waterlogging treatments. In the figure, WTL indicates the waterlogging tolerant line (PI408105A) and WSL indicates the waterlogging susceptible line (S99-2281). The arrows in (B–D) indicate aerenchyma cells in the root. (E) Shows lipid peroxidation (MDA) in the root and star marks indicate a significantly difference between control and treatment at P < 0.05 or P < 0.01. Data is the average ± standard error (n = 3).
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Figure 7: The images of root section (A–D) and MDA (E) in root after 10 days waterlogging treatments. In the figure, WTL indicates the waterlogging tolerant line (PI408105A) and WSL indicates the waterlogging susceptible line (S99-2281). The arrows in (B–D) indicate aerenchyma cells in the root. (E) Shows lipid peroxidation (MDA) in the root and star marks indicate a significantly difference between control and treatment at P < 0.05 or P < 0.01. Data is the average ± standard error (n = 3).

Mentions: In waterlogged and submergence conditions, soil pore space was covered with water so plants faced hypoxia. Under normal conditions, plants can uptake oxygen from the soil through the root, which is used in energy generative by the process of respiration. However, under flooding conditions, plants cannot take up oxygen from soil. In order to facilitate oxygen uptake, the constitution of plant root cells changes to adapt stress conditions. For these reasons, we inspected the root cells of soybean plants after 10 days of waterlogging treatment. As shown in Figure 7, aerenchyma cells were observed in WTL compared to WSL, and the number of aerenchyma cells and size of aerenchyma cells were higher in the waterlogging treatment than the control at WTL (Figures 7A,B), while development of aerenchyma cell was not different between the control and treatment in WSL (Figures 7C,D). Therefore, aerenchyma cells were better developed in WTL than in WSL. To support the difference in development of aerenchyma cells, we analyzed lipid peroxidation (MDA) (Figure 7E) and no change was found between WTL and WSL without waterlogging treatment. Nonetheless, WTL showed greater levels of lipid peroxidation than the WSL under waterlogging stress.


Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance.

Kim YH, Hwang SJ, Waqas M, Khan AL, Lee JH, Lee JD, Nguyen HT, Lee IJ - Front Plant Sci (2015)

The images of root section (A–D) and MDA (E) in root after 10 days waterlogging treatments. In the figure, WTL indicates the waterlogging tolerant line (PI408105A) and WSL indicates the waterlogging susceptible line (S99-2281). The arrows in (B–D) indicate aerenchyma cells in the root. (E) Shows lipid peroxidation (MDA) in the root and star marks indicate a significantly difference between control and treatment at P < 0.05 or P < 0.01. Data is the average ± standard error (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4585003&req=5

Figure 7: The images of root section (A–D) and MDA (E) in root after 10 days waterlogging treatments. In the figure, WTL indicates the waterlogging tolerant line (PI408105A) and WSL indicates the waterlogging susceptible line (S99-2281). The arrows in (B–D) indicate aerenchyma cells in the root. (E) Shows lipid peroxidation (MDA) in the root and star marks indicate a significantly difference between control and treatment at P < 0.05 or P < 0.01. Data is the average ± standard error (n = 3).
Mentions: In waterlogged and submergence conditions, soil pore space was covered with water so plants faced hypoxia. Under normal conditions, plants can uptake oxygen from the soil through the root, which is used in energy generative by the process of respiration. However, under flooding conditions, plants cannot take up oxygen from soil. In order to facilitate oxygen uptake, the constitution of plant root cells changes to adapt stress conditions. For these reasons, we inspected the root cells of soybean plants after 10 days of waterlogging treatment. As shown in Figure 7, aerenchyma cells were observed in WTL compared to WSL, and the number of aerenchyma cells and size of aerenchyma cells were higher in the waterlogging treatment than the control at WTL (Figures 7A,B), while development of aerenchyma cell was not different between the control and treatment in WSL (Figures 7C,D). Therefore, aerenchyma cells were better developed in WTL than in WSL. To support the difference in development of aerenchyma cells, we analyzed lipid peroxidation (MDA) (Figure 7E) and no change was found between WTL and WSL without waterlogging treatment. Nonetheless, WTL showed greater levels of lipid peroxidation than the WSL under waterlogging stress.

Bottom Line: Endogenous hormones also showed significant differences between WTL and WSL.The ratio of total GA and ABA was significantly higher in WTL than in WSL.Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biosciences, Kyungpook National University Daegu, South Korea ; Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri Columbia, MO, USA.

ABSTRACT
Waterlogged condition due to flooding is one of the major abiotic stresses that drastically affect the soybean growth and yield around the world. As a result, many breeders have focused on the development of waterlogging tolerance in soybean varieties, and thus, several tolerant varieties were developed. However, the physiological mechanism of waterlogging tolerance is not yet fully understood. We particularly studied the endogenous hormones regulation during waterlogging in two contrasting soybean genotypes. According to our results, adventitious roots were better developed in the waterlogging tolerant line (WTL) than in the waterlogging susceptible line (WSL). Endogenous hormones also showed significant differences between WTL and WSL. The ethylene production ratio was higher in WTL than in WSL, and methionine was higher in WTL than in WSL. Other endogenous abscisic acid (ABA) contents were lower in WTL than in WSL. Conversely, gibberellic acid (GA) showed a tendency to be high in WTL, especially the levels of the bioactive GA4. The ratio of total GA and ABA was significantly higher in WTL than in WSL. Anatomical study of the root revealed that aerenchyma cells in the stele were better developed in WTL than in WSL.

No MeSH data available.