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Influence of Rhizoctonia solani and Trichoderma spp. in growth of bean (Phaseolus vulgaris L.) and in the induction of plant defense-related genes.

Mayo S, Gutiérrez S, Malmierca MG, Lorenzana A, Campelo MP, Hermosa R, Casquero PA - Front Plant Sci (2015)

Bottom Line: Squalene and ergosterol production differences were found among the Trichoderma isolates, T019 showing the highest values for both compounds.T. harzianum T019 shows a positive effect on the level of resistance of bean plants to R. solani.This strain induces the expression of plant defense-related genes and produces a higher level of ergosterol, indicating its ability to grow at a higher rate in the soil, which would explain its positive effects on plant growth and defense in the presence of the pathogen.

View Article: PubMed Central - PubMed

Affiliation: Research Group of Engineering and Sustainable Agriculture, Natural Resources Institute, University of León León, Spain.

ABSTRACT
Many Trichoderma species are well-known for their ability to promote plant growth and defense. We study how the interaction of bean plants with R. solani and/or Trichoderma affect the plants growth and the level of expression of defense-related genes. Trichoderma isolates were evaluated in vitro for their potential to antagonize R. solani. Bioassays were performed in climatic chambers and development of the plants was evaluated. The effect of Trichoderma treatment and/or R. solani infection on the expression of bean defense-related genes was analyzed by real-time PCR and the production of ergosterol and squalene was quantified. In vitro growth inhibition of R. solani was between 86 and 58%. In in vivo assays, the bean plants treated with Trichoderma harzianum T019 always had an increased size respect to control and the plants treated with this isolate did not decrease their size in presence of R. solani. The interaction of plants with R. solani and/or Trichoderma affects the level of expression of seven defense-related genes. Squalene and ergosterol production differences were found among the Trichoderma isolates, T019 showing the highest values for both compounds. T. harzianum T019 shows a positive effect on the level of resistance of bean plants to R. solani. This strain induces the expression of plant defense-related genes and produces a higher level of ergosterol, indicating its ability to grow at a higher rate in the soil, which would explain its positive effects on plant growth and defense in the presence of the pathogen.

No MeSH data available.


Related in: MedlinePlus

Differences in growth of bean plants. CC plant control; RC plant with R. solani, CT019 plant with Trichoderma T019; RT019 plant with R. solani and Trichoderma T019.
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Figure 4: Differences in growth of bean plants. CC plant control; RC plant with R. solani, CT019 plant with Trichoderma T019; RT019 plant with R. solani and Trichoderma T019.

Mentions: Based on these results, the Trichoderma isolate T019 was selected for further studies since it showed the best positive effects on plant phenotype among all the analyzed isolates (Figure 4).


Influence of Rhizoctonia solani and Trichoderma spp. in growth of bean (Phaseolus vulgaris L.) and in the induction of plant defense-related genes.

Mayo S, Gutiérrez S, Malmierca MG, Lorenzana A, Campelo MP, Hermosa R, Casquero PA - Front Plant Sci (2015)

Differences in growth of bean plants. CC plant control; RC plant with R. solani, CT019 plant with Trichoderma T019; RT019 plant with R. solani and Trichoderma T019.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584982&req=5

Figure 4: Differences in growth of bean plants. CC plant control; RC plant with R. solani, CT019 plant with Trichoderma T019; RT019 plant with R. solani and Trichoderma T019.
Mentions: Based on these results, the Trichoderma isolate T019 was selected for further studies since it showed the best positive effects on plant phenotype among all the analyzed isolates (Figure 4).

Bottom Line: Squalene and ergosterol production differences were found among the Trichoderma isolates, T019 showing the highest values for both compounds.T. harzianum T019 shows a positive effect on the level of resistance of bean plants to R. solani.This strain induces the expression of plant defense-related genes and produces a higher level of ergosterol, indicating its ability to grow at a higher rate in the soil, which would explain its positive effects on plant growth and defense in the presence of the pathogen.

View Article: PubMed Central - PubMed

Affiliation: Research Group of Engineering and Sustainable Agriculture, Natural Resources Institute, University of León León, Spain.

ABSTRACT
Many Trichoderma species are well-known for their ability to promote plant growth and defense. We study how the interaction of bean plants with R. solani and/or Trichoderma affect the plants growth and the level of expression of defense-related genes. Trichoderma isolates were evaluated in vitro for their potential to antagonize R. solani. Bioassays were performed in climatic chambers and development of the plants was evaluated. The effect of Trichoderma treatment and/or R. solani infection on the expression of bean defense-related genes was analyzed by real-time PCR and the production of ergosterol and squalene was quantified. In vitro growth inhibition of R. solani was between 86 and 58%. In in vivo assays, the bean plants treated with Trichoderma harzianum T019 always had an increased size respect to control and the plants treated with this isolate did not decrease their size in presence of R. solani. The interaction of plants with R. solani and/or Trichoderma affects the level of expression of seven defense-related genes. Squalene and ergosterol production differences were found among the Trichoderma isolates, T019 showing the highest values for both compounds. T. harzianum T019 shows a positive effect on the level of resistance of bean plants to R. solani. This strain induces the expression of plant defense-related genes and produces a higher level of ergosterol, indicating its ability to grow at a higher rate in the soil, which would explain its positive effects on plant growth and defense in the presence of the pathogen.

No MeSH data available.


Related in: MedlinePlus