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The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus

Expression analyses of SsuHsfs in different tissues. The expression of 27 SsuHsfs in shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC) from S. suchowensis. The amplification cycle used was 35 for six asterisk (*) labeled genes and 30 for all the other SsuHsfs and the SsuActin reference control. One experiment representative for three biological replicate experiments was shown in here. Primers used for RT-PCR are listed in Table S2.
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Figure 7: Expression analyses of SsuHsfs in different tissues. The expression of 27 SsuHsfs in shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC) from S. suchowensis. The amplification cycle used was 35 for six asterisk (*) labeled genes and 30 for all the other SsuHsfs and the SsuActin reference control. One experiment representative for three biological replicate experiments was shown in here. Primers used for RT-PCR are listed in Table S2.

Mentions: To identify the spatial and temporal expression patterns of the SsuHsfs, RT-PCR was performed on the 27 SsuHsfs in nine different tissues of S. suchowensis: the shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC). Most SsuHsfs showed distinct tissue expression patterns. As shown in Figure 7, some genes had tissue-specific expression patterns; for example, SsuHsf-B3 was highly expressed in the secondary stem and xylem, SsuHsf-B4c was highly expressed in the shoot tip and phloem, and SsuHsf-A7a was highly expressed in the mature leaf. Interestingly, SsuHsf-A9 was specifically expressed in the female catkin.


The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Expression analyses of SsuHsfs in different tissues. The expression of 27 SsuHsfs in shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC) from S. suchowensis. The amplification cycle used was 35 for six asterisk (*) labeled genes and 30 for all the other SsuHsfs and the SsuActin reference control. One experiment representative for three biological replicate experiments was shown in here. Primers used for RT-PCR are listed in Table S2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584977&req=5

Figure 7: Expression analyses of SsuHsfs in different tissues. The expression of 27 SsuHsfs in shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC) from S. suchowensis. The amplification cycle used was 35 for six asterisk (*) labeled genes and 30 for all the other SsuHsfs and the SsuActin reference control. One experiment representative for three biological replicate experiments was shown in here. Primers used for RT-PCR are listed in Table S2.
Mentions: To identify the spatial and temporal expression patterns of the SsuHsfs, RT-PCR was performed on the 27 SsuHsfs in nine different tissues of S. suchowensis: the shoot tip (ST), young leaf (YL), mature leaf (ML), primary stem (PS), secondary stem (SS), phloem (Phl), xylem (Xyl), root (R), and female catkin (FC). Most SsuHsfs showed distinct tissue expression patterns. As shown in Figure 7, some genes had tissue-specific expression patterns; for example, SsuHsf-B3 was highly expressed in the secondary stem and xylem, SsuHsf-B4c was highly expressed in the shoot tip and phloem, and SsuHsf-A7a was highly expressed in the mature leaf. Interestingly, SsuHsf-A9 was specifically expressed in the female catkin.

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus