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The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus

Multiple sequence alignment of the DBD domains of the SsuHsf proteins. The secondary structures of the DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. α-helices and β-sheets were marked using cylindrical tubes and block arrows, respectively.
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Figure 4: Multiple sequence alignment of the DBD domains of the SsuHsf proteins. The secondary structures of the DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. α-helices and β-sheets were marked using cylindrical tubes and block arrows, respectively.

Mentions: The modular structures of Hsfs have been studied thoroughly in some model plants (Nover et al., 2001; Scharf et al., 2012). The known information on functional domains of AtHsfs makes it possible to identify similar domains in the SsuHsfs. As shown in Table 4, five conserved domains (DBD, HR-A/B, NLS, NES, and AHA) were identified by sequence alignment and their positions in the proteins. The conserved DBD comprised three α-helices (α1–3) and four β-sheets (β1–4) (Figure 4). It has been reported that NES and NLS domains are essential for shuttling Hsfs between the nucleus and cytoplasm (Scharf et al., 2012), and the majority of the SsuHsfs showed the presence of a NES and/or NLS domain. Furthermore, AHA motifs were identified in most of the Class A SsuHsfs. However, we were unable to predict putative AHA motifs in the Class B and C proteins (Table 4).


The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Multiple sequence alignment of the DBD domains of the SsuHsf proteins. The secondary structures of the DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. α-helices and β-sheets were marked using cylindrical tubes and block arrows, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584977&req=5

Figure 4: Multiple sequence alignment of the DBD domains of the SsuHsf proteins. The secondary structures of the DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. α-helices and β-sheets were marked using cylindrical tubes and block arrows, respectively.
Mentions: The modular structures of Hsfs have been studied thoroughly in some model plants (Nover et al., 2001; Scharf et al., 2012). The known information on functional domains of AtHsfs makes it possible to identify similar domains in the SsuHsfs. As shown in Table 4, five conserved domains (DBD, HR-A/B, NLS, NES, and AHA) were identified by sequence alignment and their positions in the proteins. The conserved DBD comprised three α-helices (α1–3) and four β-sheets (β1–4) (Figure 4). It has been reported that NES and NLS domains are essential for shuttling Hsfs between the nucleus and cytoplasm (Scharf et al., 2012), and the majority of the SsuHsfs showed the presence of a NES and/or NLS domain. Furthermore, AHA motifs were identified in most of the Class A SsuHsfs. However, we were unable to predict putative AHA motifs in the Class B and C proteins (Table 4).

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus