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The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus

Gene structures of SsuHsf genes. Boxes represent exons and lines represent introns. The numbers indicate the splicing phases of the SsuHsfs: 0, phase 0; 1, phase 1; and 2, phase 2.
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Figure 2: Gene structures of SsuHsf genes. Boxes represent exons and lines represent introns. The numbers indicate the splicing phases of the SsuHsfs: 0, phase 0; 1, phase 1; and 2, phase 2.

Mentions: To evaluate the structural diversity of the SsuHsf genes, the full-length cDNA sequences were compared with the corresponding genomic DNA sequences to determine the numbers and positions of exons and introns within each gene (Figure 2). Exon/intron structural divergence within a gene family plays a critical role during evolution. In general, paralogous genes are highly conserved in gene structure and this conservation is sufficient to reveal their evolutionary relationships (Hardison, 1996). Most SsuHsf genes included only one intron, except for SsuHsf-A1a, SsuHsf-B2a, and SsuHsf-B4a, which included two introns. The intron phases were remarkably well-conserved among family members (Figure 2).


The heat shock factor gene family in Salix suchowensis: a genome-wide survey and expression profiling during development and abiotic stresses.

Zhang J, Li Y, Jia HX, Li JB, Huang J, Lu MZ, Hu JJ - Front Plant Sci (2015)

Gene structures of SsuHsf genes. Boxes represent exons and lines represent introns. The numbers indicate the splicing phases of the SsuHsfs: 0, phase 0; 1, phase 1; and 2, phase 2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584977&req=5

Figure 2: Gene structures of SsuHsf genes. Boxes represent exons and lines represent introns. The numbers indicate the splicing phases of the SsuHsfs: 0, phase 0; 1, phase 1; and 2, phase 2.
Mentions: To evaluate the structural diversity of the SsuHsf genes, the full-length cDNA sequences were compared with the corresponding genomic DNA sequences to determine the numbers and positions of exons and introns within each gene (Figure 2). Exon/intron structural divergence within a gene family plays a critical role during evolution. In general, paralogous genes are highly conserved in gene structure and this conservation is sufficient to reveal their evolutionary relationships (Hardison, 1996). Most SsuHsf genes included only one intron, except for SsuHsf-A1a, SsuHsf-B2a, and SsuHsf-B4a, which included two introns. The intron phases were remarkably well-conserved among family members (Figure 2).

Bottom Line: Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses.The results demonstrated that the SsuHsfs were involved in abiotic stress responses.Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China ; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of the shrub willow Salix suchowensis was fully sequenced. In this study, a total of 27 non-redundant Hsf genes were identified from the S. suchowensis genome. Phylogenetic analysis revealed that the members of the SsuHsf family can be divided into three groups (class A, B, and C) based on their structural characteristics. Promoter analysis indicated that the SsuHsfs promoters included various cis-acting elements related to hormone and/or stress responses. Furthermore, the expression profiles of 27 SsuHsfs were analyzed in different tissues and under various stresses (heat, drought, salt, and ABA treatment) using RT-PCR. The results demonstrated that the SsuHsfs were involved in abiotic stress responses. Our results contribute to a better understanding of the complexity of the SsuHsf gene family, and will facilitate functional characterization in future studies.

No MeSH data available.


Related in: MedlinePlus