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Aging and chronic administration of serotonin-selective reuptake inhibitor citalopram upregulate Sirt4 gene expression in the preoptic area of male mice.

Wong DW, Soga T, Parhar IS - Front Genet (2015)

Bottom Line: Moreover, the number of SIRT4 immuno-reactive neurons increased with aging in the medial septum area (12 weeks = 1.00 ± 0.15 vs. 36 weeks = 1.68 ± 0.14 vs. 52 weeks = 1.54 ± 0.11, p < 0.05).In contrast, the number of sirt4-immunopositive cells did not show a statistically significant change with CIT treatment, suggesting that the increase in sirt4 mRNA levels may occur in cells in which sirt4 is already being expressed.Taken together, these studies suggest that CIT treatment and the process of aging utilize the serotonergic system to up-regulate SIRT4 in the POA as a common pathway to deregulate social cognitive and reproductive functions.

View Article: PubMed Central - PubMed

Affiliation: Brain Research Institute, School of Medicine and Health Sciences, Monash University Malaysia Selangor, Malaysia.

ABSTRACT
Sexual dysfunction and cognitive deficits are markers of the aging process. Mammalian sirtuins (SIRT), encoded by sirt 1-7 genes, are known as aging molecules which are sensitive to serotonin (5-hydroxytryptamine, 5-HT). Whether the 5-HT system regulates SIRT in the preoptic area (POA), which could affect reproduction and cognition has not been examined. Therefore, this study was designed to examine the effects of citalopram (CIT, 10 mg/kg for 4 weeks), a potent selective-serotonin reuptake inhibitor and aging on SIRT expression in the POA of male mice using real-time PCR and immunocytochemistry. Age-related increases of sirt1, sirt4, sirt5, and sirt7 mRNA levels were observed in the POA of 52 weeks old mice. Furthermore, 4 weeks of chronic CIT treatment started at 8 weeks of age also increased sirt2 and sirt4 mRNA expression in the POA. Moreover, the number of SIRT4 immuno-reactive neurons increased with aging in the medial septum area (12 weeks = 1.00 ± 0.15 vs. 36 weeks = 1.68 ± 0.14 vs. 52 weeks = 1.54 ± 0.11, p < 0.05). In contrast, the number of sirt4-immunopositive cells did not show a statistically significant change with CIT treatment, suggesting that the increase in sirt4 mRNA levels may occur in cells in which sirt4 is already being expressed. Taken together, these studies suggest that CIT treatment and the process of aging utilize the serotonergic system to up-regulate SIRT4 in the POA as a common pathway to deregulate social cognitive and reproductive functions.

No MeSH data available.


Related in: MedlinePlus

Nicotinamide adenine dinucleotide (NAD) biosynthethic enzymes were not affected by chronic citalopram (CIT) treatment or aging. CIT was administered at 10 mg/kg/bodyweight daily in male mice for 4 weeks duration. Data are expressed as mean ± SEM. Statistical analysis was carried out using one way analysis of variance (12 weeks control, n = 9; 12 weeks CIT, n = 9) aging animals used were 36 weeks (n = 8) and 52 weeks (n = 10).
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Figure 3: Nicotinamide adenine dinucleotide (NAD) biosynthethic enzymes were not affected by chronic citalopram (CIT) treatment or aging. CIT was administered at 10 mg/kg/bodyweight daily in male mice for 4 weeks duration. Data are expressed as mean ± SEM. Statistical analysis was carried out using one way analysis of variance (12 weeks control, n = 9; 12 weeks CIT, n = 9) aging animals used were 36 weeks (n = 8) and 52 weeks (n = 10).

Mentions: CIT and aging had no effect on nam and nmnat-1 expression in the POA (Figure 3).


Aging and chronic administration of serotonin-selective reuptake inhibitor citalopram upregulate Sirt4 gene expression in the preoptic area of male mice.

Wong DW, Soga T, Parhar IS - Front Genet (2015)

Nicotinamide adenine dinucleotide (NAD) biosynthethic enzymes were not affected by chronic citalopram (CIT) treatment or aging. CIT was administered at 10 mg/kg/bodyweight daily in male mice for 4 weeks duration. Data are expressed as mean ± SEM. Statistical analysis was carried out using one way analysis of variance (12 weeks control, n = 9; 12 weeks CIT, n = 9) aging animals used were 36 weeks (n = 8) and 52 weeks (n = 10).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584971&req=5

Figure 3: Nicotinamide adenine dinucleotide (NAD) biosynthethic enzymes were not affected by chronic citalopram (CIT) treatment or aging. CIT was administered at 10 mg/kg/bodyweight daily in male mice for 4 weeks duration. Data are expressed as mean ± SEM. Statistical analysis was carried out using one way analysis of variance (12 weeks control, n = 9; 12 weeks CIT, n = 9) aging animals used were 36 weeks (n = 8) and 52 weeks (n = 10).
Mentions: CIT and aging had no effect on nam and nmnat-1 expression in the POA (Figure 3).

Bottom Line: Moreover, the number of SIRT4 immuno-reactive neurons increased with aging in the medial septum area (12 weeks = 1.00 ± 0.15 vs. 36 weeks = 1.68 ± 0.14 vs. 52 weeks = 1.54 ± 0.11, p < 0.05).In contrast, the number of sirt4-immunopositive cells did not show a statistically significant change with CIT treatment, suggesting that the increase in sirt4 mRNA levels may occur in cells in which sirt4 is already being expressed.Taken together, these studies suggest that CIT treatment and the process of aging utilize the serotonergic system to up-regulate SIRT4 in the POA as a common pathway to deregulate social cognitive and reproductive functions.

View Article: PubMed Central - PubMed

Affiliation: Brain Research Institute, School of Medicine and Health Sciences, Monash University Malaysia Selangor, Malaysia.

ABSTRACT
Sexual dysfunction and cognitive deficits are markers of the aging process. Mammalian sirtuins (SIRT), encoded by sirt 1-7 genes, are known as aging molecules which are sensitive to serotonin (5-hydroxytryptamine, 5-HT). Whether the 5-HT system regulates SIRT in the preoptic area (POA), which could affect reproduction and cognition has not been examined. Therefore, this study was designed to examine the effects of citalopram (CIT, 10 mg/kg for 4 weeks), a potent selective-serotonin reuptake inhibitor and aging on SIRT expression in the POA of male mice using real-time PCR and immunocytochemistry. Age-related increases of sirt1, sirt4, sirt5, and sirt7 mRNA levels were observed in the POA of 52 weeks old mice. Furthermore, 4 weeks of chronic CIT treatment started at 8 weeks of age also increased sirt2 and sirt4 mRNA expression in the POA. Moreover, the number of SIRT4 immuno-reactive neurons increased with aging in the medial septum area (12 weeks = 1.00 ± 0.15 vs. 36 weeks = 1.68 ± 0.14 vs. 52 weeks = 1.54 ± 0.11, p < 0.05). In contrast, the number of sirt4-immunopositive cells did not show a statistically significant change with CIT treatment, suggesting that the increase in sirt4 mRNA levels may occur in cells in which sirt4 is already being expressed. Taken together, these studies suggest that CIT treatment and the process of aging utilize the serotonergic system to up-regulate SIRT4 in the POA as a common pathway to deregulate social cognitive and reproductive functions.

No MeSH data available.


Related in: MedlinePlus