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CD36 is involved in oleic acid detection by the murine olfactory system.

Oberland S, Ackels T, Gaab S, Pelz T, Spehr J, Spehr M, Neuhaus EM - Front Cell Neurosci (2015)

Bottom Line: In accordance with the described roles of CD36 as fatty acid receptor or co-receptor in other sensory systems, the number of olfactory neurons responding to oleic acid, a major milk component, in Ca(2+) imaging experiments is drastically reduced in young CD36 knock-out mice.Strikingly, we also observe marked age-dependent changes in CD36 localization, which is prominently present in the ciliary compartment only during the suckling period.Our results support the involvement of CD36 in fatty acid detection by the mammalian olfactory system.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology and Toxicology, University Hospital Jena, Friedrich-Schiller-University Jena Jena, Germany ; Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin Berlin, Germany ; Freie Universität-Berlin, Fachbereich Biologie, Chemie und Pharmazie Berlin, Germany.

ABSTRACT
Olfactory signals influence food intake in a variety of species. To maximize the chances of finding a source of calories, an animal's preference for fatty foods and triglycerides already becomes apparent during olfactory food search behavior. However, the molecular identity of both receptors and ligands mediating olfactory-dependent fatty acid recognition are, so far, undescribed. We here describe that a subset of olfactory sensory neurons expresses the fatty acid receptor CD36 and demonstrate a receptor-like localization of CD36 in olfactory cilia by STED microscopy. CD36-positive olfactory neurons share olfaction-specific transduction elements and project to numerous glomeruli in the ventral olfactory bulb. In accordance with the described roles of CD36 as fatty acid receptor or co-receptor in other sensory systems, the number of olfactory neurons responding to oleic acid, a major milk component, in Ca(2+) imaging experiments is drastically reduced in young CD36 knock-out mice. Strikingly, we also observe marked age-dependent changes in CD36 localization, which is prominently present in the ciliary compartment only during the suckling period. Our results support the involvement of CD36 in fatty acid detection by the mammalian olfactory system.

No MeSH data available.


Related in: MedlinePlus

CD36 distribution in the olfactory epithelium. (A) Widefield microscopy image of a cryosection (P8) immunostained for CD36. CD36 expressing neurons are scattered along the olfactory epithelium. (nc, nasal cavity; s, septum; t, turbinate; ob, olfactory bulb). (B) Magnification of the boxed area shown in (A). Immunostaining for CD36 shows expression in a subset of olfactory neurons. (C) Stitching of fluorescence stereo microscopy images of a whole-mount turbinate preparation immunostained for CD36 (P8). Olfactory knobs of CD36 expressing neurons are detected as small dots all along the surface of the epithelium lining the turbinates Scale bar: 500 μm (A), 50 μm (B).
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Figure 2: CD36 distribution in the olfactory epithelium. (A) Widefield microscopy image of a cryosection (P8) immunostained for CD36. CD36 expressing neurons are scattered along the olfactory epithelium. (nc, nasal cavity; s, septum; t, turbinate; ob, olfactory bulb). (B) Magnification of the boxed area shown in (A). Immunostaining for CD36 shows expression in a subset of olfactory neurons. (C) Stitching of fluorescence stereo microscopy images of a whole-mount turbinate preparation immunostained for CD36 (P8). Olfactory knobs of CD36 expressing neurons are detected as small dots all along the surface of the epithelium lining the turbinates Scale bar: 500 μm (A), 50 μm (B).

Mentions: Most olfactory sensory neurons that express a specific odorant receptor are restricted to one of four zones of the olfactory epithelium in the nasal cavity in the mouse (Ressler et al., 1993; Vassar et al., 1993). Also TAAR genes are expressed in unique populations of sensory neurons spread in single zones of the olfactory epithelium (Liberles and Buck, 2006). By contrast, CD36 expressing cells were not confined to a specific epithelial region (Figure 2). Analysis of cryosections from both rostral and caudal areas of the olfactory epithelium (Figure 2A) and whole-mount turbinate preparations (Figure 2C) revealed non-zonal CD36 expression along the septum and on all turbinates.


CD36 is involved in oleic acid detection by the murine olfactory system.

Oberland S, Ackels T, Gaab S, Pelz T, Spehr J, Spehr M, Neuhaus EM - Front Cell Neurosci (2015)

CD36 distribution in the olfactory epithelium. (A) Widefield microscopy image of a cryosection (P8) immunostained for CD36. CD36 expressing neurons are scattered along the olfactory epithelium. (nc, nasal cavity; s, septum; t, turbinate; ob, olfactory bulb). (B) Magnification of the boxed area shown in (A). Immunostaining for CD36 shows expression in a subset of olfactory neurons. (C) Stitching of fluorescence stereo microscopy images of a whole-mount turbinate preparation immunostained for CD36 (P8). Olfactory knobs of CD36 expressing neurons are detected as small dots all along the surface of the epithelium lining the turbinates Scale bar: 500 μm (A), 50 μm (B).
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4584952&req=5

Figure 2: CD36 distribution in the olfactory epithelium. (A) Widefield microscopy image of a cryosection (P8) immunostained for CD36. CD36 expressing neurons are scattered along the olfactory epithelium. (nc, nasal cavity; s, septum; t, turbinate; ob, olfactory bulb). (B) Magnification of the boxed area shown in (A). Immunostaining for CD36 shows expression in a subset of olfactory neurons. (C) Stitching of fluorescence stereo microscopy images of a whole-mount turbinate preparation immunostained for CD36 (P8). Olfactory knobs of CD36 expressing neurons are detected as small dots all along the surface of the epithelium lining the turbinates Scale bar: 500 μm (A), 50 μm (B).
Mentions: Most olfactory sensory neurons that express a specific odorant receptor are restricted to one of four zones of the olfactory epithelium in the nasal cavity in the mouse (Ressler et al., 1993; Vassar et al., 1993). Also TAAR genes are expressed in unique populations of sensory neurons spread in single zones of the olfactory epithelium (Liberles and Buck, 2006). By contrast, CD36 expressing cells were not confined to a specific epithelial region (Figure 2). Analysis of cryosections from both rostral and caudal areas of the olfactory epithelium (Figure 2A) and whole-mount turbinate preparations (Figure 2C) revealed non-zonal CD36 expression along the septum and on all turbinates.

Bottom Line: In accordance with the described roles of CD36 as fatty acid receptor or co-receptor in other sensory systems, the number of olfactory neurons responding to oleic acid, a major milk component, in Ca(2+) imaging experiments is drastically reduced in young CD36 knock-out mice.Strikingly, we also observe marked age-dependent changes in CD36 localization, which is prominently present in the ciliary compartment only during the suckling period.Our results support the involvement of CD36 in fatty acid detection by the mammalian olfactory system.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology and Toxicology, University Hospital Jena, Friedrich-Schiller-University Jena Jena, Germany ; Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin Berlin, Germany ; Freie Universität-Berlin, Fachbereich Biologie, Chemie und Pharmazie Berlin, Germany.

ABSTRACT
Olfactory signals influence food intake in a variety of species. To maximize the chances of finding a source of calories, an animal's preference for fatty foods and triglycerides already becomes apparent during olfactory food search behavior. However, the molecular identity of both receptors and ligands mediating olfactory-dependent fatty acid recognition are, so far, undescribed. We here describe that a subset of olfactory sensory neurons expresses the fatty acid receptor CD36 and demonstrate a receptor-like localization of CD36 in olfactory cilia by STED microscopy. CD36-positive olfactory neurons share olfaction-specific transduction elements and project to numerous glomeruli in the ventral olfactory bulb. In accordance with the described roles of CD36 as fatty acid receptor or co-receptor in other sensory systems, the number of olfactory neurons responding to oleic acid, a major milk component, in Ca(2+) imaging experiments is drastically reduced in young CD36 knock-out mice. Strikingly, we also observe marked age-dependent changes in CD36 localization, which is prominently present in the ciliary compartment only during the suckling period. Our results support the involvement of CD36 in fatty acid detection by the mammalian olfactory system.

No MeSH data available.


Related in: MedlinePlus