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Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins.

Boerhout E, Vrieling M, Benedictus L, Daemen I, Ravesloot L, Rutten V, Nuijten P, van Strijp J, Koets A, Eisenberg S - Vet. Res. (2015)

Bottom Line: It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection.The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate.Furthermore, the harmful effects of Efb and leukotoxin LukMF' on host-defense were neutralized by serum antibodies in a route-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Ruminant Research and Development, MSD Animal Health, Wim de Körverstraat 35, 5830, AA, Boxmeer, The Netherlands. eveline.boerhout@merck.com.

ABSTRACT
Vaccines against S. aureus bovine mastitis are scarce and show limited protection only. All currently available vaccines are applied via the parenteral (usually intramuscular) route. It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection. Hence, in the present study, immunization via mucosal (intranasal; IN), intramuscular (triangle of the neck; IM), intramammary (IMM) and subcutaneous (suspensory ligament; SC) routes were analyzed for their effects on the quantity of the antibody responses in serum and milk as well as the neutralizing capacity of the antibodies within serum. The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate. The highest titer increases for both Efb and LukM specific IgG1 and IgG2 antibody levels in serum and milk were observed following SC/SC immunizations. Furthermore, the harmful effects of Efb and leukotoxin LukMF' on host-defense were neutralized by serum antibodies in a route-dependent manner. SC/SC immunization resulted in a significant increase in the neutralizing capacity of serum antibodies towards Efb and LukMF', shown by increased phagocytosis of S. aureus and increased viability of bovine leukocytes. Therefore, a SC immunization route should be considered when aiming to optimize humoral immunity against S. aureus mastitis in cattle.

No MeSH data available.


Related in: MedlinePlus

Pre-immunization titers of isotype specific antibodies directed against Efb and LukM. Initial Efb (A) and LukM (B) specific antibody titers in serum, milk, saliva and nasal secretion samples were measured by ELISA. Each bar represents mean ± SEM (n = 16).
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Fig1: Pre-immunization titers of isotype specific antibodies directed against Efb and LukM. Initial Efb (A) and LukM (B) specific antibody titers in serum, milk, saliva and nasal secretion samples were measured by ELISA. Each bar represents mean ± SEM (n = 16).

Mentions: Prior to immunization, in all cows Efb and LukM specific antibodies were detected in serum, milk, saliva and nasal secretions, with the exception of Efb specific IgG1 and IgG2, and LukM specific IgA antibodies in milk (Figure 1). The levels of these initial titers were not significantly different between groups (Additional file 1).Figure 1


Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins.

Boerhout E, Vrieling M, Benedictus L, Daemen I, Ravesloot L, Rutten V, Nuijten P, van Strijp J, Koets A, Eisenberg S - Vet. Res. (2015)

Pre-immunization titers of isotype specific antibodies directed against Efb and LukM. Initial Efb (A) and LukM (B) specific antibody titers in serum, milk, saliva and nasal secretion samples were measured by ELISA. Each bar represents mean ± SEM (n = 16).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4584483&req=5

Fig1: Pre-immunization titers of isotype specific antibodies directed against Efb and LukM. Initial Efb (A) and LukM (B) specific antibody titers in serum, milk, saliva and nasal secretion samples were measured by ELISA. Each bar represents mean ± SEM (n = 16).
Mentions: Prior to immunization, in all cows Efb and LukM specific antibodies were detected in serum, milk, saliva and nasal secretions, with the exception of Efb specific IgG1 and IgG2, and LukM specific IgA antibodies in milk (Figure 1). The levels of these initial titers were not significantly different between groups (Additional file 1).Figure 1

Bottom Line: It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection.The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate.Furthermore, the harmful effects of Efb and leukotoxin LukMF' on host-defense were neutralized by serum antibodies in a route-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Ruminant Research and Development, MSD Animal Health, Wim de Körverstraat 35, 5830, AA, Boxmeer, The Netherlands. eveline.boerhout@merck.com.

ABSTRACT
Vaccines against S. aureus bovine mastitis are scarce and show limited protection only. All currently available vaccines are applied via the parenteral (usually intramuscular) route. It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection. Hence, in the present study, immunization via mucosal (intranasal; IN), intramuscular (triangle of the neck; IM), intramammary (IMM) and subcutaneous (suspensory ligament; SC) routes were analyzed for their effects on the quantity of the antibody responses in serum and milk as well as the neutralizing capacity of the antibodies within serum. The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate. The highest titer increases for both Efb and LukM specific IgG1 and IgG2 antibody levels in serum and milk were observed following SC/SC immunizations. Furthermore, the harmful effects of Efb and leukotoxin LukMF' on host-defense were neutralized by serum antibodies in a route-dependent manner. SC/SC immunization resulted in a significant increase in the neutralizing capacity of serum antibodies towards Efb and LukMF', shown by increased phagocytosis of S. aureus and increased viability of bovine leukocytes. Therefore, a SC immunization route should be considered when aiming to optimize humoral immunity against S. aureus mastitis in cattle.

No MeSH data available.


Related in: MedlinePlus