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The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.

Baker KS, Burnett E, McGregor H, Deheer-Graham A, Boinett C, Langridge GC, Wailan AM, Cain AK, Thomson NR, Russell JE, Parkhill J - Genome Med (2015)

Bottom Line: To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains.Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content.This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.

ABSTRACT
Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

No MeSH data available.


Related in: MedlinePlus

Rarefaction curves for pan- (above) and core- (below) genome sizes by genus
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Fig2: Rarefaction curves for pan- (above) and core- (below) genome sizes by genus

Mentions: To verify the robustness of the Collection, as well as add value, provide further metadata, and facilitate the development of selection criteria for ongoing studies, 370 strains (representing 291 equivalence groups), mostly representative of the collection (TablesĀ 1, 2, Additional file 1: Table S2 and Additional file 7: Table S3) were whole genome sequenced. Some analyses of these genomes are briefly reported here, and more detail is given in the Additional file 6: Supplementary Material.Fig. 2


The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.

Baker KS, Burnett E, McGregor H, Deheer-Graham A, Boinett C, Langridge GC, Wailan AM, Cain AK, Thomson NR, Russell JE, Parkhill J - Genome Med (2015)

Rarefaction curves for pan- (above) and core- (below) genome sizes by genus
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4584482&req=5

Fig2: Rarefaction curves for pan- (above) and core- (below) genome sizes by genus
Mentions: To verify the robustness of the Collection, as well as add value, provide further metadata, and facilitate the development of selection criteria for ongoing studies, 370 strains (representing 291 equivalence groups), mostly representative of the collection (TablesĀ 1, 2, Additional file 1: Table S2 and Additional file 7: Table S3) were whole genome sequenced. Some analyses of these genomes are briefly reported here, and more detail is given in the Additional file 6: Supplementary Material.Fig. 2

Bottom Line: To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains.Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content.This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.

ABSTRACT
Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

No MeSH data available.


Related in: MedlinePlus