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The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.

Baker KS, Burnett E, McGregor H, Deheer-Graham A, Boinett C, Langridge GC, Wailan AM, Cain AK, Thomson NR, Russell JE, Parkhill J - Genome Med (2015)

Bottom Line: To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains.Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content.This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.

ABSTRACT
Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

No MeSH data available.


Related in: MedlinePlus

Metadata available for the Collection strains by genus, including year on original vial (a) and number of plasmids (b)
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Fig1: Metadata available for the Collection strains by genus, including year on original vial (a) and number of plasmids (b)

Mentions: The demographic features (e.g. place, person, time) and clinical details of pathogen infection are often crucial in the interpretation of genotypic and phenotypic analyses on the isolated pathogen. Although many of these details are available for the Collection strains, this metadata is incomplete and somewhat imperfect. The diverse geographical origins of the collection “including Europe, Malta, the Middle East, northern Russia, India and North America” has been reported [11], but were not available for individual strains. Metadata held at the NCTC showed the strains originated from diverse clinical specimens, e.g. stool, urine, blood, antral washes, cerebrospinal fluid, but the clinical syndrome, e.g. meningitis, pneumonia, hepatitis and cholecystitis, or patient/supplier name were also alternatively recorded (Additional file 1: Table S2). This ‘Origin’ information was only available for approximately one quarter (n = 150) of the strains. Contrastingly however, the large majority (92 %, 628 of 683) of strains had a date or year noted on the original vial (Additional file 1: Table S2). When these dates were stratified by genus, a unique time signature emerged, perhaps reflecting E.G.D. Murray’s changing research interests over time (Fig. 1a). Notably, these dates were presumed to be the date of isolation for the strains, but could also represent date of strain receipt, or some other event. Overall however, the novel analyses presented in this study largely support the original metadata demonstrating that it is, if imperfect, robust.Fig. 1


The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.

Baker KS, Burnett E, McGregor H, Deheer-Graham A, Boinett C, Langridge GC, Wailan AM, Cain AK, Thomson NR, Russell JE, Parkhill J - Genome Med (2015)

Metadata available for the Collection strains by genus, including year on original vial (a) and number of plasmids (b)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4584482&req=5

Fig1: Metadata available for the Collection strains by genus, including year on original vial (a) and number of plasmids (b)
Mentions: The demographic features (e.g. place, person, time) and clinical details of pathogen infection are often crucial in the interpretation of genotypic and phenotypic analyses on the isolated pathogen. Although many of these details are available for the Collection strains, this metadata is incomplete and somewhat imperfect. The diverse geographical origins of the collection “including Europe, Malta, the Middle East, northern Russia, India and North America” has been reported [11], but were not available for individual strains. Metadata held at the NCTC showed the strains originated from diverse clinical specimens, e.g. stool, urine, blood, antral washes, cerebrospinal fluid, but the clinical syndrome, e.g. meningitis, pneumonia, hepatitis and cholecystitis, or patient/supplier name were also alternatively recorded (Additional file 1: Table S2). This ‘Origin’ information was only available for approximately one quarter (n = 150) of the strains. Contrastingly however, the large majority (92 %, 628 of 683) of strains had a date or year noted on the original vial (Additional file 1: Table S2). When these dates were stratified by genus, a unique time signature emerged, perhaps reflecting E.G.D. Murray’s changing research interests over time (Fig. 1a). Notably, these dates were presumed to be the date of isolation for the strains, but could also represent date of strain receipt, or some other event. Overall however, the novel analyses presented in this study largely support the original metadata demonstrating that it is, if imperfect, robust.Fig. 1

Bottom Line: To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains.Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content.This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.

ABSTRACT
Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

No MeSH data available.


Related in: MedlinePlus