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Human papillomavirus types in non-cervical high-grade intraepithelial neoplasias and invasive carcinomas from San Luis Potosí, Mexico: a retrospective cross-sectional study.

Magaña-León C, Oros C, López-Revilla R - Infect. Agents Cancer (2015)

Bottom Line: Viral infections and the burden of high-grade intraepithelial neoplasias (HIN) and invasive carcinomas (IC) associated to infections by human papillomavirus (HPV) types may be prevented by type-specific anti-HPV vaccines.Five high-risk viral types (HPV16, 45, 59, 33 and 35) and two low-risk types (HPV11 and 54) infect one third of the non-cervical HIN and IC included.Most infections are by a single HPV high-risk type, the most prevalent one being HPV16.

View Article: PubMed Central - PubMed

Affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, S.L.P. Mexico.

ABSTRACT

Background: Viral infections and the burden of high-grade intraepithelial neoplasias (HIN) and invasive carcinomas (IC) associated to infections by human papillomavirus (HPV) types may be prevented by type-specific anti-HPV vaccines. This study determined the prevalence of HPV types in non-cervical HIN and IC diagnosed from 1999 to 2011 at a general hospital in San Luis Potosí, Mexico.

Methods: Review of the 67 formaldehyde-fixed paraffin-embedded non-cervical specimens initially diagnosed as HIN (n = 28) or IC (n = 39) confirmed the presence of tumor tissue in 63 of them and changed the diagnosis of 24 from HIN to low-grade intraepithelial neoplasias, that were excluded from the study. HPV DNA was detected with the SPF10-DNA enzyme immunoassay in the 39 cases included, and viral types in the HPV-positive tumors were identified with the INNO-LiPA linear probe array.

Results: Among the cases included, four HIN were located in the vagina (n = 3) and vulva (n = 1), and 35 IC in the oral cavity (n = 19), penis (n = 8), vagina (n = 7) and vulva (n = 1). There were 13 HPV-positive cases from the vagina (n = 7), vulva (n = 1), penis (n = 1) and oral cavity (n = 1). The viral types identified were the high-risk types HPV16 in the vagina (n = 3) and vulva (n = 3), HPV45 in the vagina (n = 2), HPV59 in the vagina (n = 1) and penis (n = 1), HPV33 in the vagina (n = 1),and HPV35 in the tongue(n = 1); and the low-risk types HPV54 in the vagina (n = 1), and HPV11 in the vulva (n = 1).

Conclusions: Five high-risk viral types (HPV16, 45, 59, 33 and 35) and two low-risk types (HPV11 and 54) infect one third of the non-cervical HIN and IC included. Most infections are by a single HPV high-risk type, the most prevalent one being HPV16. Vagina is the most frequent location of the HPV-positive tumors. Vaccination against HPV16 and HPV18 could have prevented around half of the HPV-positive tumors.

No MeSH data available.


Related in: MedlinePlus

Stages of the study. Histopathological review showed that four of the 67 initial paraffin blocks lacked tumor tissue and were discarded. Of the 63 acceptable paraffin blocks, 28 were excluded because their initial diagnoses changed from HIN to LIN. DNA extracted from the paraffin blocks of the 39 cases included (35 IC and four HIN) was subjected to the SPF10-DEIA assay. The 13 HPV-positive cases were further analyzed with the INNO-LiPA assay to identify the viral types
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Fig1: Stages of the study. Histopathological review showed that four of the 67 initial paraffin blocks lacked tumor tissue and were discarded. Of the 63 acceptable paraffin blocks, 28 were excluded because their initial diagnoses changed from HIN to LIN. DNA extracted from the paraffin blocks of the 39 cases included (35 IC and four HIN) was subjected to the SPF10-DEIA assay. The 13 HPV-positive cases were further analyzed with the INNO-LiPA assay to identify the viral types

Mentions: The study was carried out in five steps (Fig. 1): 1) 67 formalin-fixed paraffin-embedded (FFPE) blocks from non-cervical tumors with initial HIN or IC diagnoses were retrieved from the Pathology Department of Hospital Central Ignacio Morones Prieto; 2) sections were obtained by the sandwich method and final diagnoses were performed at the ICO Pathology Reference Laboratory; 3) cases with final IC or HIN histopathological diagnoses were included, and those lacking tumor tissue or with final diagnoses of low-grade intraepithelial neoplasia (LIN) were excluded; 4) SPF10-DEIA was performed to identify HPV-positive and -negative tumors in the cases included; 5) viral types in the HPV-positive cases were identified with the INNO-LiPA assay.Fig. 1


Human papillomavirus types in non-cervical high-grade intraepithelial neoplasias and invasive carcinomas from San Luis Potosí, Mexico: a retrospective cross-sectional study.

Magaña-León C, Oros C, López-Revilla R - Infect. Agents Cancer (2015)

Stages of the study. Histopathological review showed that four of the 67 initial paraffin blocks lacked tumor tissue and were discarded. Of the 63 acceptable paraffin blocks, 28 were excluded because their initial diagnoses changed from HIN to LIN. DNA extracted from the paraffin blocks of the 39 cases included (35 IC and four HIN) was subjected to the SPF10-DEIA assay. The 13 HPV-positive cases were further analyzed with the INNO-LiPA assay to identify the viral types
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4584459&req=5

Fig1: Stages of the study. Histopathological review showed that four of the 67 initial paraffin blocks lacked tumor tissue and were discarded. Of the 63 acceptable paraffin blocks, 28 were excluded because their initial diagnoses changed from HIN to LIN. DNA extracted from the paraffin blocks of the 39 cases included (35 IC and four HIN) was subjected to the SPF10-DEIA assay. The 13 HPV-positive cases were further analyzed with the INNO-LiPA assay to identify the viral types
Mentions: The study was carried out in five steps (Fig. 1): 1) 67 formalin-fixed paraffin-embedded (FFPE) blocks from non-cervical tumors with initial HIN or IC diagnoses were retrieved from the Pathology Department of Hospital Central Ignacio Morones Prieto; 2) sections were obtained by the sandwich method and final diagnoses were performed at the ICO Pathology Reference Laboratory; 3) cases with final IC or HIN histopathological diagnoses were included, and those lacking tumor tissue or with final diagnoses of low-grade intraepithelial neoplasia (LIN) were excluded; 4) SPF10-DEIA was performed to identify HPV-positive and -negative tumors in the cases included; 5) viral types in the HPV-positive cases were identified with the INNO-LiPA assay.Fig. 1

Bottom Line: Viral infections and the burden of high-grade intraepithelial neoplasias (HIN) and invasive carcinomas (IC) associated to infections by human papillomavirus (HPV) types may be prevented by type-specific anti-HPV vaccines.Five high-risk viral types (HPV16, 45, 59, 33 and 35) and two low-risk types (HPV11 and 54) infect one third of the non-cervical HIN and IC included.Most infections are by a single HPV high-risk type, the most prevalent one being HPV16.

View Article: PubMed Central - PubMed

Affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, S.L.P. Mexico.

ABSTRACT

Background: Viral infections and the burden of high-grade intraepithelial neoplasias (HIN) and invasive carcinomas (IC) associated to infections by human papillomavirus (HPV) types may be prevented by type-specific anti-HPV vaccines. This study determined the prevalence of HPV types in non-cervical HIN and IC diagnosed from 1999 to 2011 at a general hospital in San Luis Potosí, Mexico.

Methods: Review of the 67 formaldehyde-fixed paraffin-embedded non-cervical specimens initially diagnosed as HIN (n = 28) or IC (n = 39) confirmed the presence of tumor tissue in 63 of them and changed the diagnosis of 24 from HIN to low-grade intraepithelial neoplasias, that were excluded from the study. HPV DNA was detected with the SPF10-DNA enzyme immunoassay in the 39 cases included, and viral types in the HPV-positive tumors were identified with the INNO-LiPA linear probe array.

Results: Among the cases included, four HIN were located in the vagina (n = 3) and vulva (n = 1), and 35 IC in the oral cavity (n = 19), penis (n = 8), vagina (n = 7) and vulva (n = 1). There were 13 HPV-positive cases from the vagina (n = 7), vulva (n = 1), penis (n = 1) and oral cavity (n = 1). The viral types identified were the high-risk types HPV16 in the vagina (n = 3) and vulva (n = 3), HPV45 in the vagina (n = 2), HPV59 in the vagina (n = 1) and penis (n = 1), HPV33 in the vagina (n = 1),and HPV35 in the tongue(n = 1); and the low-risk types HPV54 in the vagina (n = 1), and HPV11 in the vulva (n = 1).

Conclusions: Five high-risk viral types (HPV16, 45, 59, 33 and 35) and two low-risk types (HPV11 and 54) infect one third of the non-cervical HIN and IC included. Most infections are by a single HPV high-risk type, the most prevalent one being HPV16. Vagina is the most frequent location of the HPV-positive tumors. Vaccination against HPV16 and HPV18 could have prevented around half of the HPV-positive tumors.

No MeSH data available.


Related in: MedlinePlus