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The effects of increasing PGE2 on translocation of labeled albumin into rat brain.

Messripour M, Mesripour A, Mashayekhie FJ - Res Pharm Sci (2015 Mar-Apr)

Bottom Line: Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented.The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant.Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran.

ABSTRACT
Under pathophysiological conditions, infiltration of leukocyte plays a key role in the progression of the neuroinflammatory reaction in the CNS. Prostaglandin E2 (PGE2) is known to accumulate at lesion sites of the post-ischemic brain. Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented. Therefore, the direct effect of increasing PGE2 infusion on translocation of labeled albumin into the brain was assessed. Under anesthesia rats were drilled stereo-taxicaly a burr hole in the right forebrain and PGE2 was infused into the forebrain and the hole was occluded. The animals were then injected with fluorescent labeled albumin (FA), via internal right jugular vein and decapitated at different infusion time points. The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant. The fluorescence intensities measured in the right and left forebrain hemispheres of the control group (0.0 μg PGE2) were almost identical. Four hours after infusion of PGE2 at doses higher than 250 μg, fluorescence intensity increased in the right forebrain supernatant, even if it was not statistically significant. The fluorescence intensity was detectable in the brain supernatant 4 h after infusion of PGE2 in doses higher than 250 μg PGE2. The highest fluorescence intensity was 16 h after infusion of 500 μg PGE2, which returned to near control values after 48 h. Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

No MeSH data available.


Related in: MedlinePlus

Dose response curve of PGE2 with respect to the fluorescence intensities. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via their internal right jugular vein and PGE2 at dose ranges from 0 to 500 μg were infused into the rat right brain hemisphere (see Method section), and the fluorescence intensities were measured 16 h after infusion at right (-●-) and left (-■-) brain supenatant.
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Figure 2: Dose response curve of PGE2 with respect to the fluorescence intensities. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via their internal right jugular vein and PGE2 at dose ranges from 0 to 500 μg were infused into the rat right brain hemisphere (see Method section), and the fluorescence intensities were measured 16 h after infusion at right (-●-) and left (-■-) brain supenatant.

Mentions: Fig. 2 compares the fluorescence intensities measured in the suprnatants prepared from right and left brain hemispheres 16 h after infusion of different doses of PGE2 (62.5, 125, 250 and 500 μg). As it can be seen the fluorescence intensities are significantly higher in the suprnatants prepared from right forebrain (P<0.05). The results indicated that translocation of FALB is dose dependent with respect to infeusion of PGE2, noticeably FALB would be augmented with doses of PGE2 higher than 125 μg.


The effects of increasing PGE2 on translocation of labeled albumin into rat brain.

Messripour M, Mesripour A, Mashayekhie FJ - Res Pharm Sci (2015 Mar-Apr)

Dose response curve of PGE2 with respect to the fluorescence intensities. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via their internal right jugular vein and PGE2 at dose ranges from 0 to 500 μg were infused into the rat right brain hemisphere (see Method section), and the fluorescence intensities were measured 16 h after infusion at right (-●-) and left (-■-) brain supenatant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584457&req=5

Figure 2: Dose response curve of PGE2 with respect to the fluorescence intensities. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via their internal right jugular vein and PGE2 at dose ranges from 0 to 500 μg were infused into the rat right brain hemisphere (see Method section), and the fluorescence intensities were measured 16 h after infusion at right (-●-) and left (-■-) brain supenatant.
Mentions: Fig. 2 compares the fluorescence intensities measured in the suprnatants prepared from right and left brain hemispheres 16 h after infusion of different doses of PGE2 (62.5, 125, 250 and 500 μg). As it can be seen the fluorescence intensities are significantly higher in the suprnatants prepared from right forebrain (P<0.05). The results indicated that translocation of FALB is dose dependent with respect to infeusion of PGE2, noticeably FALB would be augmented with doses of PGE2 higher than 125 μg.

Bottom Line: Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented.The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant.Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran.

ABSTRACT
Under pathophysiological conditions, infiltration of leukocyte plays a key role in the progression of the neuroinflammatory reaction in the CNS. Prostaglandin E2 (PGE2) is known to accumulate at lesion sites of the post-ischemic brain. Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented. Therefore, the direct effect of increasing PGE2 infusion on translocation of labeled albumin into the brain was assessed. Under anesthesia rats were drilled stereo-taxicaly a burr hole in the right forebrain and PGE2 was infused into the forebrain and the hole was occluded. The animals were then injected with fluorescent labeled albumin (FA), via internal right jugular vein and decapitated at different infusion time points. The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant. The fluorescence intensities measured in the right and left forebrain hemispheres of the control group (0.0 μg PGE2) were almost identical. Four hours after infusion of PGE2 at doses higher than 250 μg, fluorescence intensity increased in the right forebrain supernatant, even if it was not statistically significant. The fluorescence intensity was detectable in the brain supernatant 4 h after infusion of PGE2 in doses higher than 250 μg PGE2. The highest fluorescence intensity was 16 h after infusion of 500 μg PGE2, which returned to near control values after 48 h. Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

No MeSH data available.


Related in: MedlinePlus