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The effects of increasing PGE2 on translocation of labeled albumin into rat brain.

Messripour M, Mesripour A, Mashayekhie FJ - Res Pharm Sci (2015 Mar-Apr)

Bottom Line: Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented.The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant.Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran.

ABSTRACT
Under pathophysiological conditions, infiltration of leukocyte plays a key role in the progression of the neuroinflammatory reaction in the CNS. Prostaglandin E2 (PGE2) is known to accumulate at lesion sites of the post-ischemic brain. Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented. Therefore, the direct effect of increasing PGE2 infusion on translocation of labeled albumin into the brain was assessed. Under anesthesia rats were drilled stereo-taxicaly a burr hole in the right forebrain and PGE2 was infused into the forebrain and the hole was occluded. The animals were then injected with fluorescent labeled albumin (FA), via internal right jugular vein and decapitated at different infusion time points. The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant. The fluorescence intensities measured in the right and left forebrain hemispheres of the control group (0.0 μg PGE2) were almost identical. Four hours after infusion of PGE2 at doses higher than 250 μg, fluorescence intensity increased in the right forebrain supernatant, even if it was not statistically significant. The fluorescence intensity was detectable in the brain supernatant 4 h after infusion of PGE2 in doses higher than 250 μg PGE2. The highest fluorescence intensity was 16 h after infusion of 500 μg PGE2, which returned to near control values after 48 h. Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

No MeSH data available.


Related in: MedlinePlus

The time course of translocation of fluorescent labeled albumin. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via internal right jugular vein and PGE2 at doses of 500 μg was infused into the rat right brain hemisphere (see Method section), The fluorescence intensities were measured at infusion time points of 4, 8, 16, 24, and 48 h after infusion in right (-●-) and left (-■-) forebrain supenatant of treatment groups and right (-○-) and left (-□-) sham groups.
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Figure 1: The time course of translocation of fluorescent labeled albumin. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via internal right jugular vein and PGE2 at doses of 500 μg was infused into the rat right brain hemisphere (see Method section), The fluorescence intensities were measured at infusion time points of 4, 8, 16, 24, and 48 h after infusion in right (-●-) and left (-■-) forebrain supenatant of treatment groups and right (-○-) and left (-□-) sham groups.

Mentions: Fig. 1 shows the time course of translocation of FALB after 500 μg PGE2 infusion. The values of fluorescence intensities increased in the treatment groups 16 and 24 h after infusion compared to corresponding sham values (P<0.05). Four hours after perfusion, the levels of tissue fluorescence intensities increased greatly in right forebrain compared with the left forebrain supernatant.


The effects of increasing PGE2 on translocation of labeled albumin into rat brain.

Messripour M, Mesripour A, Mashayekhie FJ - Res Pharm Sci (2015 Mar-Apr)

The time course of translocation of fluorescent labeled albumin. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via internal right jugular vein and PGE2 at doses of 500 μg was infused into the rat right brain hemisphere (see Method section), The fluorescence intensities were measured at infusion time points of 4, 8, 16, 24, and 48 h after infusion in right (-●-) and left (-■-) forebrain supenatant of treatment groups and right (-○-) and left (-□-) sham groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584457&req=5

Figure 1: The time course of translocation of fluorescent labeled albumin. Animals were injected with 1 ml fluorescein labeled albumin (100 μg albumin/ml), via internal right jugular vein and PGE2 at doses of 500 μg was infused into the rat right brain hemisphere (see Method section), The fluorescence intensities were measured at infusion time points of 4, 8, 16, 24, and 48 h after infusion in right (-●-) and left (-■-) forebrain supenatant of treatment groups and right (-○-) and left (-□-) sham groups.
Mentions: Fig. 1 shows the time course of translocation of FALB after 500 μg PGE2 infusion. The values of fluorescence intensities increased in the treatment groups 16 and 24 h after infusion compared to corresponding sham values (P<0.05). Four hours after perfusion, the levels of tissue fluorescence intensities increased greatly in right forebrain compared with the left forebrain supernatant.

Bottom Line: Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented.The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant.Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran.

ABSTRACT
Under pathophysiological conditions, infiltration of leukocyte plays a key role in the progression of the neuroinflammatory reaction in the CNS. Prostaglandin E2 (PGE2) is known to accumulate at lesion sites of the post-ischemic brain. Although post-ischemic treatments with cyclooxygenase-2 inhibitors reduce blood-brain barrier (BBB) leukocyte infiltration, the direct effect of PGE2 on BBB has not been fully implemented. Therefore, the direct effect of increasing PGE2 infusion on translocation of labeled albumin into the brain was assessed. Under anesthesia rats were drilled stereo-taxicaly a burr hole in the right forebrain and PGE2 was infused into the forebrain and the hole was occluded. The animals were then injected with fluorescent labeled albumin (FA), via internal right jugular vein and decapitated at different infusion time points. The forebrain was removed and each forebrain hemisphere was homogenized and fluorescence intensities were measured in the supernatant. The fluorescence intensities measured in the right and left forebrain hemispheres of the control group (0.0 μg PGE2) were almost identical. Four hours after infusion of PGE2 at doses higher than 250 μg, fluorescence intensity increased in the right forebrain supernatant, even if it was not statistically significant. The fluorescence intensity was detectable in the brain supernatant 4 h after infusion of PGE2 in doses higher than 250 μg PGE2. The highest fluorescence intensity was 16 h after infusion of 500 μg PGE2, which returned to near control values after 48 h. Increased fluorescence intensity in the brain following PGE2 infusion is concluded to be associated with disruption of the BBB.

No MeSH data available.


Related in: MedlinePlus