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Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line.

Dana N, Javanmard ShH, Rafiee L - Res Pharm Sci (2015 Mar-Apr)

Bottom Line: VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05).Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism.Further studies are needed to confirm these results.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. PPE was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10-450 μg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 μg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) assay. VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.

No MeSH data available.


Related in: MedlinePlus

ELISA demonstrated decreased levels of VEGF in the culture medium at 300 and 400 μg/ml after 24 h incubation. Data are presented as means ± SD of three experiments. *; P<0:05.
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Figure 5: ELISA demonstrated decreased levels of VEGF in the culture medium at 300 and 400 μg/ml after 24 h incubation. Data are presented as means ± SD of three experiments. *; P<0:05.

Mentions: As shown in Fig. 5, treatment of HUVECs with PPE decreased the VEGF concentration in the culture medium. VEGF concentration was significantly decreased at 300 and 400 μg/ml concentrations of PPE (P<0.05) at 200 μg/ml dose of PPE, however, decreasing VEGF was not significant.


Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line.

Dana N, Javanmard ShH, Rafiee L - Res Pharm Sci (2015 Mar-Apr)

ELISA demonstrated decreased levels of VEGF in the culture medium at 300 and 400 μg/ml after 24 h incubation. Data are presented as means ± SD of three experiments. *; P<0:05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584450&req=5

Figure 5: ELISA demonstrated decreased levels of VEGF in the culture medium at 300 and 400 μg/ml after 24 h incubation. Data are presented as means ± SD of three experiments. *; P<0:05.
Mentions: As shown in Fig. 5, treatment of HUVECs with PPE decreased the VEGF concentration in the culture medium. VEGF concentration was significantly decreased at 300 and 400 μg/ml concentrations of PPE (P<0.05) at 200 μg/ml dose of PPE, however, decreasing VEGF was not significant.

Bottom Line: VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05).Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism.Further studies are needed to confirm these results.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. PPE was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10-450 μg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 μg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) assay. VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.

No MeSH data available.


Related in: MedlinePlus