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Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line.

Dana N, Javanmard ShH, Rafiee L - Res Pharm Sci (2015 Mar-Apr)

Bottom Line: VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05).Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism.Further studies are needed to confirm these results.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. PPE was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10-450 μg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 μg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) assay. VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.

No MeSH data available.


Related in: MedlinePlus

The effects of PPE on cell viability. As detailed in Materials and methods, A; C554 HUVECs, B; B16F10 melanoma cells were treated with PPE and the viability of cells was determined by the MTT assay. The data are expressed as the percentage of cell viability compare to control.
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Figure 1: The effects of PPE on cell viability. As detailed in Materials and methods, A; C554 HUVECs, B; B16F10 melanoma cells were treated with PPE and the viability of cells was determined by the MTT assay. The data are expressed as the percentage of cell viability compare to control.

Mentions: As shown in Fig. 1A, PPE treatment of HUVECs resulted in 6 and 17% decrease in cell viability at concentrations 10–450 μg/ml of PPE, respectively indicating that PPE had minimal toxic effect on these cells. PPE showed toxic effects on B16F10 cells at its lowest (1%) up to its highest (61%) concentrations dose dependently (Fig. 1B). The calculated IC50 value for the PPE against B16F10 cells was found to be 310.21 indicating that the PEE was toxic against B16F10 cells.


Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line.

Dana N, Javanmard ShH, Rafiee L - Res Pharm Sci (2015 Mar-Apr)

The effects of PPE on cell viability. As detailed in Materials and methods, A; C554 HUVECs, B; B16F10 melanoma cells were treated with PPE and the viability of cells was determined by the MTT assay. The data are expressed as the percentage of cell viability compare to control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584450&req=5

Figure 1: The effects of PPE on cell viability. As detailed in Materials and methods, A; C554 HUVECs, B; B16F10 melanoma cells were treated with PPE and the viability of cells was determined by the MTT assay. The data are expressed as the percentage of cell viability compare to control.
Mentions: As shown in Fig. 1A, PPE treatment of HUVECs resulted in 6 and 17% decrease in cell viability at concentrations 10–450 μg/ml of PPE, respectively indicating that PPE had minimal toxic effect on these cells. PPE showed toxic effects on B16F10 cells at its lowest (1%) up to its highest (61%) concentrations dose dependently (Fig. 1B). The calculated IC50 value for the PPE against B16F10 cells was found to be 310.21 indicating that the PEE was toxic against B16F10 cells.

Bottom Line: VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05).Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism.Further studies are needed to confirm these results.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. PPE was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10-450 μg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 μg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) assay. VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of PPE treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.

No MeSH data available.


Related in: MedlinePlus