Limits...
Comparison of antispasmodic effects of Dracocephalum kotschyi essential oil, limonene and α-terpineol.

Sadraei H, Asghari G, Kasiri F - Res Pharm Sci (2015 Mar-Apr)

Bottom Line: Dracocephalum kotschyi is an essential oil containing plant found in Iran.Their inhibitory effects were also concentration dependent.Limonene contribute a major role in inhibitory effect of the essential oil while α-terpineol has weak antispasmodic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
Dracocephalum kotschyi is an essential oil containing plant found in Iran. In Iranian traditional medicine, D. kotschyi has been used as antispasmodic and analgesic but so far there is no pharmacological report about its antispasmodic activity. Therefore, the objective of this research was to study antispasmodic activity of the essential oil of D. kotschyi and two of its constituents namely limonene and α-terpineol. The essential oil was obtained from aerial parts of D. kotschyi using hydrodistillation method. The main components found in the essential oil were α-pinene (10%), neral (11%), geraniol (10%), α-citral (12%), limonene (9%) and α-terpineol (1.1%). For antispasmodic studies, a portion of rat ileum was suspended under 1 g tension in Tyrode's solution at 37 °C and gassed with O2. Effect of the D. kotschyi essential oil, limonene and α-terpineol were studied on ileum contractions induced by KCl (80 mM), acetylcholine (ACh, 500 nM) and electrical field stimulation (EFS). The essential oil, in a concentration dependent manner inhibited the response to KCl (IC50=51 ± 8.7 nl/ml), ACh (IC50=19 ± 2.7 nl/ml) and EFS (IC50=15 ± 0.5 nl/ml). Limonene and α-terpineol showed same pattern of inhibitory effect on ileum contraction. Their inhibitory effects were also concentration dependent. However, limonene was more potent than the essential oil while the α-terpineol was less potent than either limonene or the essential oil. From this experiment it was concluded that D. kotschyi essential oil has inhibitory effect on ileum contractions. Limonene contribute a major role in inhibitory effect of the essential oil while α-terpineol has weak antispasmodic activity.

No MeSH data available.


Related in: MedlinePlus

Cumulative effect of limonene, α-terpineol and Dracocephalum kotschyi essential oil on tension development to potassium chloride (KCl, 80 mM) in isolated ileum of rats. Ordinate scale: ileum contraction expressed as % of initial control response. Abscissa scale: log10 concentration of drugs (limonene, α-terpineol or D. kotschyi essential oil). Lines drawn through the points, using two fold increments in concentration. The points are mean and the vertical bars show the SEM (n=6). The oscillation in the control group is not statistically significant (ANOVA). Stars shows statistical differences between each drug concentration with its corresponding vehicle treated control. Keys: *P<0.05, **P<0.01, ***P<0.001 (Student's t-test). Maximum concentration of the vehicle (DMSO) in the bath was 0.64%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4584449&req=5

Figure 1: Cumulative effect of limonene, α-terpineol and Dracocephalum kotschyi essential oil on tension development to potassium chloride (KCl, 80 mM) in isolated ileum of rats. Ordinate scale: ileum contraction expressed as % of initial control response. Abscissa scale: log10 concentration of drugs (limonene, α-terpineol or D. kotschyi essential oil). Lines drawn through the points, using two fold increments in concentration. The points are mean and the vertical bars show the SEM (n=6). The oscillation in the control group is not statistically significant (ANOVA). Stars shows statistical differences between each drug concentration with its corresponding vehicle treated control. Keys: *P<0.05, **P<0.01, ***P<0.001 (Student's t-test). Maximum concentration of the vehicle (DMSO) in the bath was 0.64%.

Mentions: D. kotschyi essential oil, limonene and α-terpineol (8 nl/ml -128 nl/ml) concentration dependently reduced contractile responses to KCl. At its highest used concentration the contractile response to KCl was totally removed (Fig. 1). At bath concentration of 64 nl/ml limonene removed the contractile response to KCl while the essential oil and α-terpineol only inhibited the response by 60% and 79% respectively. The IC50 values are given in Table 1 for comparison. After washing the tissue with fresh Tyrode's solution, the contractile response to KCl was gradually restored. There was no statistically difference in the vehicle treated time match control tissues over the course of studies (ANOVA).


Comparison of antispasmodic effects of Dracocephalum kotschyi essential oil, limonene and α-terpineol.

Sadraei H, Asghari G, Kasiri F - Res Pharm Sci (2015 Mar-Apr)

Cumulative effect of limonene, α-terpineol and Dracocephalum kotschyi essential oil on tension development to potassium chloride (KCl, 80 mM) in isolated ileum of rats. Ordinate scale: ileum contraction expressed as % of initial control response. Abscissa scale: log10 concentration of drugs (limonene, α-terpineol or D. kotschyi essential oil). Lines drawn through the points, using two fold increments in concentration. The points are mean and the vertical bars show the SEM (n=6). The oscillation in the control group is not statistically significant (ANOVA). Stars shows statistical differences between each drug concentration with its corresponding vehicle treated control. Keys: *P<0.05, **P<0.01, ***P<0.001 (Student's t-test). Maximum concentration of the vehicle (DMSO) in the bath was 0.64%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4584449&req=5

Figure 1: Cumulative effect of limonene, α-terpineol and Dracocephalum kotschyi essential oil on tension development to potassium chloride (KCl, 80 mM) in isolated ileum of rats. Ordinate scale: ileum contraction expressed as % of initial control response. Abscissa scale: log10 concentration of drugs (limonene, α-terpineol or D. kotschyi essential oil). Lines drawn through the points, using two fold increments in concentration. The points are mean and the vertical bars show the SEM (n=6). The oscillation in the control group is not statistically significant (ANOVA). Stars shows statistical differences between each drug concentration with its corresponding vehicle treated control. Keys: *P<0.05, **P<0.01, ***P<0.001 (Student's t-test). Maximum concentration of the vehicle (DMSO) in the bath was 0.64%.
Mentions: D. kotschyi essential oil, limonene and α-terpineol (8 nl/ml -128 nl/ml) concentration dependently reduced contractile responses to KCl. At its highest used concentration the contractile response to KCl was totally removed (Fig. 1). At bath concentration of 64 nl/ml limonene removed the contractile response to KCl while the essential oil and α-terpineol only inhibited the response by 60% and 79% respectively. The IC50 values are given in Table 1 for comparison. After washing the tissue with fresh Tyrode's solution, the contractile response to KCl was gradually restored. There was no statistically difference in the vehicle treated time match control tissues over the course of studies (ANOVA).

Bottom Line: Dracocephalum kotschyi is an essential oil containing plant found in Iran.Their inhibitory effects were also concentration dependent.Limonene contribute a major role in inhibitory effect of the essential oil while α-terpineol has weak antispasmodic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
Dracocephalum kotschyi is an essential oil containing plant found in Iran. In Iranian traditional medicine, D. kotschyi has been used as antispasmodic and analgesic but so far there is no pharmacological report about its antispasmodic activity. Therefore, the objective of this research was to study antispasmodic activity of the essential oil of D. kotschyi and two of its constituents namely limonene and α-terpineol. The essential oil was obtained from aerial parts of D. kotschyi using hydrodistillation method. The main components found in the essential oil were α-pinene (10%), neral (11%), geraniol (10%), α-citral (12%), limonene (9%) and α-terpineol (1.1%). For antispasmodic studies, a portion of rat ileum was suspended under 1 g tension in Tyrode's solution at 37 °C and gassed with O2. Effect of the D. kotschyi essential oil, limonene and α-terpineol were studied on ileum contractions induced by KCl (80 mM), acetylcholine (ACh, 500 nM) and electrical field stimulation (EFS). The essential oil, in a concentration dependent manner inhibited the response to KCl (IC50=51 ± 8.7 nl/ml), ACh (IC50=19 ± 2.7 nl/ml) and EFS (IC50=15 ± 0.5 nl/ml). Limonene and α-terpineol showed same pattern of inhibitory effect on ileum contraction. Their inhibitory effects were also concentration dependent. However, limonene was more potent than the essential oil while the α-terpineol was less potent than either limonene or the essential oil. From this experiment it was concluded that D. kotschyi essential oil has inhibitory effect on ileum contractions. Limonene contribute a major role in inhibitory effect of the essential oil while α-terpineol has weak antispasmodic activity.

No MeSH data available.


Related in: MedlinePlus