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Nfic regulates tooth root patterning and growth.

Kim TH, Bae CH, Yang S, Park JC, Cho ES - Anat Cell Biol (2015)

Bottom Line: In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars.These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner.From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences, School of Dentistry, Chonbuk National University, Jeonju, Korea.

ABSTRACT
Molecular interactions between epithelium and mesenchyme are important for root formation. Nuclear factor I-C (Nfic) has been identified as a key regulator of root formation. However, the mechanisms of root formation and their interactions between Hertwig's epithelial root sheath (HERS) and mesenchyme remain unclear. In this study, we investigated the role of Nfic in root patterning and growth during molar root development. The molars of Nfic knockout mice exhibited an enlarged pulp chamber and apical displacement of the pulpal floor, characteristic features of taurodontism, due to delayed furcation formation. In developing molar roots of mutant mice at P14, BrdU positive cells decreased in the apical mesenchyme of the elongation region whereas those cells increased in the dental papilla of the furcation region. Whereas cytokeratin 14 and laminin were localized in HERS cells of mutant molars, Smoothened (Smo) and Gli1 were downregulated in preodontoblasts. In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars. These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner. From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

No MeSH data available.


Related in: MedlinePlus

Histology and cell proliferation analysis in the mandibular first molar of nuclear factor I-C (Nfic) knockout mice. (A-D) Comparison of elongation region in wild type (WT) and mutant (MT) mice at 8 days old (P8) and P28. (E-H) Furcation region. (I-L) BrdU labeling of proliferating cells in the apical dental mesenchyme of developing mandibular molar roots in Nfic knockout mice at P10. (M) Differential regulation of cell proliferation between the elongation and furcation region. In MT mice, cell proliferation was reduced in the elongation region but increased significantly in the furcation region. Scale bars=400 µm (A-H), 100 µm (I-L).
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Figure 2: Histology and cell proliferation analysis in the mandibular first molar of nuclear factor I-C (Nfic) knockout mice. (A-D) Comparison of elongation region in wild type (WT) and mutant (MT) mice at 8 days old (P8) and P28. (E-H) Furcation region. (I-L) BrdU labeling of proliferating cells in the apical dental mesenchyme of developing mandibular molar roots in Nfic knockout mice at P10. (M) Differential regulation of cell proliferation between the elongation and furcation region. In MT mice, cell proliferation was reduced in the elongation region but increased significantly in the furcation region. Scale bars=400 µm (A-H), 100 µm (I-L).

Mentions: To compare the histological differences between the root elongation and furcation region during root development, we observed hematoxylin and eosin stained frontal sections across the center of the mesial root and mid-furcation of mandibular first molars (Fig. 2A-H). At P8, there was no remarkable difference in the elongation region between WT and MT mice (Fig. 2A, B). In the furcation region of WT mice, thin dentin was observed, but elongated HERS without dentin was observed in MT mice (Fig. 2E, F). At P28, root length was extremely short in the elongation region of MT mice (Fig. 2C, D). In the furcation region of WT mice, relatively thick inter-radicular dentin dentin at the furcation region was formed close to the cervix, but inter-radicular dentin it was very thin and apically displaced in MT mice (Fig. 2G, H).


Nfic regulates tooth root patterning and growth.

Kim TH, Bae CH, Yang S, Park JC, Cho ES - Anat Cell Biol (2015)

Histology and cell proliferation analysis in the mandibular first molar of nuclear factor I-C (Nfic) knockout mice. (A-D) Comparison of elongation region in wild type (WT) and mutant (MT) mice at 8 days old (P8) and P28. (E-H) Furcation region. (I-L) BrdU labeling of proliferating cells in the apical dental mesenchyme of developing mandibular molar roots in Nfic knockout mice at P10. (M) Differential regulation of cell proliferation between the elongation and furcation region. In MT mice, cell proliferation was reduced in the elongation region but increased significantly in the furcation region. Scale bars=400 µm (A-H), 100 µm (I-L).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4582161&req=5

Figure 2: Histology and cell proliferation analysis in the mandibular first molar of nuclear factor I-C (Nfic) knockout mice. (A-D) Comparison of elongation region in wild type (WT) and mutant (MT) mice at 8 days old (P8) and P28. (E-H) Furcation region. (I-L) BrdU labeling of proliferating cells in the apical dental mesenchyme of developing mandibular molar roots in Nfic knockout mice at P10. (M) Differential regulation of cell proliferation between the elongation and furcation region. In MT mice, cell proliferation was reduced in the elongation region but increased significantly in the furcation region. Scale bars=400 µm (A-H), 100 µm (I-L).
Mentions: To compare the histological differences between the root elongation and furcation region during root development, we observed hematoxylin and eosin stained frontal sections across the center of the mesial root and mid-furcation of mandibular first molars (Fig. 2A-H). At P8, there was no remarkable difference in the elongation region between WT and MT mice (Fig. 2A, B). In the furcation region of WT mice, thin dentin was observed, but elongated HERS without dentin was observed in MT mice (Fig. 2E, F). At P28, root length was extremely short in the elongation region of MT mice (Fig. 2C, D). In the furcation region of WT mice, relatively thick inter-radicular dentin dentin at the furcation region was formed close to the cervix, but inter-radicular dentin it was very thin and apically displaced in MT mice (Fig. 2G, H).

Bottom Line: In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars.These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner.From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences, School of Dentistry, Chonbuk National University, Jeonju, Korea.

ABSTRACT
Molecular interactions between epithelium and mesenchyme are important for root formation. Nuclear factor I-C (Nfic) has been identified as a key regulator of root formation. However, the mechanisms of root formation and their interactions between Hertwig's epithelial root sheath (HERS) and mesenchyme remain unclear. In this study, we investigated the role of Nfic in root patterning and growth during molar root development. The molars of Nfic knockout mice exhibited an enlarged pulp chamber and apical displacement of the pulpal floor, characteristic features of taurodontism, due to delayed furcation formation. In developing molar roots of mutant mice at P14, BrdU positive cells decreased in the apical mesenchyme of the elongation region whereas those cells increased in the dental papilla of the furcation region. Whereas cytokeratin 14 and laminin were localized in HERS cells of mutant molars, Smoothened (Smo) and Gli1 were downregulated in preodontoblasts. In contrast, cytokeratin 14 and Smo were localized in the cells of the furcation region of mutant molars. These results indicate that Nfic regulates cell proliferation in the dental mesenchyme and affects the fate of HERS cells in a site-specific manner. From the results, it is suggested that Nfic is required for root patterning and growth during root morphogenesis.

No MeSH data available.


Related in: MedlinePlus